Professional Manufacturer of Biomagnetic Beads
In today’s bioscience research and medical diagnostic field, the purity and efficiency of nucleic acid extraction directly affect the accuracy and reliability of experimental results. The 160024 DNA/RNA Nucleic Acid Extraction Beads launched by Shanghai Lingjun Biotechnology Co., Ltd. is winning the favour of researchers worldwide with its excellent performance.
With an average particle size of 1.4µm, the silica-based magnetic beads of 160024 have strong magnetic properties, large adsorption capacity and high purity of extraction.
Product parameters：
| 160024 | |
| Active group | Si-OH |
| Magnetic bead matrix | Superparamagnetic silica |
| mean particle diameter | 1.4µm |
| value of saturated magnetic | 75±5emu/g |
For example, the recovery of 20ng/ul target DNA by magnetic beads is about 95% or more (Table 1).
Table 1: Recovery performance of different batches of 160024
| No. | 260/280 | 260/230 | Conc. (ng/ul) | Recovery |
| DNA | 2.09 | 5.17 | 20.00 | / |
| 2.10 | 5.01 | 19.95 | ||
| 2.14 | 5.27 | 19.90 | ||
| Average | 2.11 | 5.15 | 19.95 | |
| 22060301 | 2.29 | 3.24 | 19.15 | 96.66% |
| 2.18 | 3.33 | 19.30 | ||
| 2.16 | 3.25 | 19.40 | ||
| Average | 2.21 | 3.28 | 19.28 | |
| 23101901 | 2.26 | 3.14 | 19.25 | 95.91% |
| 2.21 | 3.25 | 18.85 | ||
| 2.21 | 3.50 | 19.30 | ||
| Average | 2.22 | 3.30 | 19.13 |
The magnetic beads are mainly used for genomic DNA extraction, DNA/RNA co-extraction, total RNA extraction, nucleic acid extraction, viral nucleic acid extraction, serum/plasma free DNA fragment extraction, especially suitable for customers who require high purity of extracted nucleic acids and strong magnetic properties of magnetic beads.
Experiment example:
E. coli DNA/RNA extraction with different sample sizes.
| No. | Sample intake | A260/A280 | A260/A230 | Conc.(ng/ul) | Yield（μg） |
| 1 | 600ul | 1.92 | 2.01 | 79.1 | 7.91 |
| 2 | 1.91 | 1.98 | 83.2 | 8.32 | |
| Avg | 1.92 | 1.998 | 81.15 | 8.12 | |
| 3 | 800ul | 1.928 | 2.105 | 106.7 | 10.67 |
| 4 | 1.938 | 2.141 | 108.8 | 10.88 | |
| Avg | 1.933 | 2.123 | 107.75 | 10.78 | |
| 5 | 1ml | 1.889 | 1.842 | 144.5 | 14.45 |
| 6 | 1.874 | 1.78 | 122.1 | 12.21 | |
| Avg | 1.882 | 1.811 | 133.3 | 13.33 |
Graph of agarose gel electrophoresis results:
Analysis of results:
The results of the ultra-micronucleic acid tester showed that:
① The values of A260/A280 were above 1.9, and the values of A260/A230 were above 2.0, and the peaks were complete and basically overlapped, which proved that the extracted products were of high purity.
② The yields of E. coli nucleic acid extracted from different sampling volumes were above 5 µg.
The results of agarose gel electrophoresis showed that:
① The nucleic acid extracted from E. coli with different sampling volume had bright bands and good integrity.
In conclusion, the extracted E. coli nucleic acid has high purity, high yield and complete electrophoretic bands.
Application example:
160024 Magnetic Beads paired with Automated Nucleic Acid Extractor LJbio32H for genomic DNA extraction from blood samples of different sources, purified to about 10 µg of genomic DNA from 200 µL of blood with high stability (results shown in Figure 1).
Therefore: Compared with traditional methods, 160024 magnetic beads have several significant advantages:
Easy operation: suitable for automatic nucleic acid extractor and fully automated workstation to achieve one-button automated extraction;
High safety: the extraction process does not require the use of toxic reagents, such as phenol, safe and non-toxic;
Stable process: the procurement and production of the process is under strict control, the beads are stable between batches and there is no discrepancy;
Suspension is good: the semi-sedimentation time of up to 20 minutes to ensure the homogeneity and consistency of the experiment. Good suspension: half-settling time up to 20 minutes, ensuring the uniformity and consistency of the experiment.
Three reasons to choose 160024 magnetic beads:
High efficiency: more than 95% recovery rate makes the experimental results more accurate and reliable
Wide range of applications: to meet the needs of a variety of sample types and extraction
Quality assurance: product quality assurance from high-tech enterprises
160024 magnetic beads are available in a wide range of packages of 2 ml/10 ml/50 ml/500 ml/1000 ml to meet the needs of laboratories of different sizes. For laboratories of different sizes.
For researchers seeking purity and efficiency in nucleic acid extraction, 160024 magnetic beads provide an ideal solution. It will help researchers save valuable time, improve the reproducibility of experiments and reduce the cost of experiments.
Ltd. adheres to the enterprise spirit of ‘quality first, step by step lead, excel, love to build the world’, and continues to provide customers with high-quality products and services.
If you are looking for a solution to improve the efficiency and purity of nucleic acid extraction, 160024 magnetic beads are the ideal choice. For more product information, please contact us.
Supplier
Shanghai Lingjun Biotechnology Co., Ltd. was established in 2016 which is a professional manufacturer of biomagnetic materials and nucleic acid extraction reagents.
We have rich experience in nucleic acid extraction and purification, protein purification, cell separation, chemiluminescence, and other technical fields.
Our products are widely used in many fields, such as medical testing, genetic testing, university research, genetic breeding, and so on. We not only provide products but also can undertake OEM, ODM, and other needs. If you have a related need, please feel free to contact us at sales01@lingjunbio.com.
