Lnjnbio الخرز المغناطيسي لمجموعة استخراج الحمض النووي الفطري 231018

(طريقة الخرزة المغناطيسية)

【مقدمة المنتج】

Fungal mycelium, sporocarp, spores, and other samples release nucleic acid under the action of lysate. The nucleic acid is separated from other impurities in the sample by the specific adsorption of magnetic beads, and then rinsed with washing buffer to remove proteins, السكريات, inorganic salts, إلخ., and finally eluted to obtain purified nucleic acid. The obtained nucleic acid products can be used for PCR, DNA detection and analysis, التهجين الجزيئي, gene mutation detection, والتسلسل.

【مزايا المنتج】

1. Core raw materials are produced by ourselves and product quality and output are guaranteed.

2. The extraction products had high purity, إنتاجية عالية ونطاقات الكهربي كاملة.

3. Magnetic bead method extraction can realize automatic operation.

4. The process of use is safe, and the product does not contain phenol, الكلوروفورم والكواشف الضارة الأخرى.

5. Because of strong versatiCore raw materials are produced by ourselves and product quality and output are guaranteed.

درجة نقاء عالية: There is no need to use toxic organic solvents such as phenol and chloroform during the extraction process. This avoids contamination from impurities and ensures high-purity DNA, which can be directly used for molecular biology experiments such as restriction enzyme digestion, تفاعل البوليميراز المتسلسل, والتسلسل.

عملية سهلة: The manual operation time is short, and it can be used in conjunction with liquid handling workstations or fully automated nucleic acid extraction instruments to achieve automated operation, making it suitable for high-throughput sample processing.

سلامة عالية: The extraction process is safe and environmentally friendly, with no involvement of hazardous reagents, protecting the health of laboratory personnel.

براعة قوية: It is applicable to various fungal samples, including mycelium, fruiting bodies, spores, etc.lity, it is suitable for a variety of fungi mycelium, sporocarp, spores and other samples for the extraction of nucleic acids.

【صورة المنتج】

【حالات تجريبية】

قضية 1:

For different fungal samples, DNA was extracted with the results as follows:

نس.	عينة	Sampe intake (ملغ)	A260/A280	A260/A230	Conc.(نانوغرام / ميكرولتر)
1	Pleurotus eryngii	20	1.84	1.63	346.6
2			1.82	1.68	344.0
3	Pleurotus ostreatus	10	1.82	1.86	278.6
4			1.87	1.91	242.2
5	White Shimeji	10	1.93	2.22	112.5
6			1.96	2.17	97.5

The electropherogram is as follows:

تحليل النتيجة: DNA from various fungal samples extracted with this product showed good purity, إنتاجية عالية ونطاقات كاملة.

قضية 2：

For different fungal samples, nucleic acids were extracted and the results were as follows:

نس.	عينة intake	A260/A280	A260/A230	Conc.(من / ماي)	أَثْمَر(ميكروغرام)
1	5 ملغ Yeast	1.948	2.095	95.8	9.58
2		1.953	2.113	100.1	10.01
متوسط		1.9505	2.104	97.95	9.795
3	5ملغ Aspergillus hyphae	1.863	1.863	176.9	17.69
4		1.862	1.868	174.35	17.435
متوسط		1.8625	1.8655	175.625	17.5625
5	20ملغ Lentinula هdodes	1.923	2.142	321.55	32.155
6		1.921	2.139	324.05	32.405
متوسط		1.922	2.1405	322.8	32.28
7	30 ملغHypsizygus marmoreus	1.94	1.993	619.35	61.935
8		1.944	1.985	626	62.6
متوسط		1.942	1.989	622.675	62.2675
9	30 ملغEryngium campestre	1.972	2.181	141.55	14.155
10		1.968	2.194	141	14.1
متوسط		1.97	2.1875	141.275	14.1275

مخطط نمط الذروة:

Yeast:

Aspergillus hyphae:

Lentinula هdodes:

Hypsizygusmarm oreus:

Eryngium campestre:

إجمالي مخطط الذروة:

يظهر الرسم الكهربائي أدناه:

م:علامة (2000 سنة مضت, 1,000 بي بي, 750 بي بي, 500 بي بي, 250 بي بي, 100 نقطة أساس);

1-2: 5 ملغ Yeast             3-4: 5ملغAspergillus hyphae        5-6: 20ملغLentinula هdodes  

7-8: 30 ملغHypsizygus marmoreus                         9-10: 30 ملغEryngium campestre

تحليل النتيجة:

وأظهرت نتائج اختبار فحص الحمض النووي فائق الصغر ذلك:

① The value of A₂₆₀ / أ₂₈₀ is from 1.86 ل 1.97, وقيمة أ₂₆₀ / أ₂₃₀ هو فوق 1.86. إن المصادفة الكاملة والأساسية لشكل الذروة تثبت النقاء العالي لمنتج الاستخلاص.

② The nucleic acid extraction yield of different fungal samples was above 9 ميكروغرام.

وأظهرت نتائج التحليل الكهربائي لهلام الاغاروز ذلك:

① Different fungal samples have bright and good integrity bands.

② In conclusion, using automatic nucleic acid extraction and purification instrument to extract different fungal samples, the extraction product has high purity, عائد مرتفع, complete electrophoretic band. The reagent has good stability when used with the instrument.

【مجال التطبيق】

Molecular Biology Research

The extracted fungal DNA can be used in various molecular biology experiments, including genome sequencing, استنساخ الجينات, تضخيم PCR, تقييد هضم الإنزيم, and library construction. These experiments help researchers gain a deep understanding of the structure, function, and evolutionary relationships of fungal genomes.

• Microbial Identification and Classification

In the field of microbiology, the reagent can be used to rapidly extract fungal DNA for the identification and classification of different fungal species. Through gene sequencing or PCR technology, the types of fungi can be accurately identified, especially for studies on microbial diversity in complex environmental samples.

• Clinical Diagnosis

In clinical medicine, fungal DNA extraction reagents can be used to detect fungal infectious diseases. By extracting fungal DNA from patient samples and combining it with PCR or sequencing technologies, fungal infections can be diagnosed quickly and accurately, providing a basis for clinical treatment.

• Food Safety Testing

In the field of food safety, the reagent can be used to detect fungal contamination in food, such as molds and yeasts. By extracting fungal DNA from food samples, rapid detection of potential pathogenic fungi can be achieved, ensuring food safety.

• Environmental Microbial Detection

في علم البيئة, fungal DNA extraction reagents can be used to analyze the diversity of fungi in environmental samples. على سبيل المثال, extracting fungal DNA from soil and water samples helps study the microbial composition and function of ecosystems.

• Forensic Identification

In the field of forensic science, the reagent can be used to extract fungal DNA from biological samples for microbial evidence analysis in cases. على سبيل المثال, detecting specific fungi in environmental samples can provide supplementary evidence for investigations.

• Bioproducts and Cell Culture

In the fields of biotechnology and pharmaceuticals, fungal DNA extraction reagents can be used to detect fungal contamination in bioproducts (such as vaccines and cell therapy products), ensuring product quality and safety.

• High-Throughput Automation Applications

The magnetic bead-based extraction reagent has good compatibility with automation and can be used in combination with automated nucleic acid extraction instruments to achieve high-throughput sample processing. This is particularly advantageous for large-scale sample testing, improving efficiency and reducing human error.

• Gene Function Research

The extracted fungal DNA can be used for gene function studies, such as investigating the functions of fungal genes through techniques like gene knockout and overexpression.

1. Molecular Diagnostics and Nucleic Acid Testing

في مجال التشخيص الجزيئي, the reagent can be used to extract fungal DNA from samples and, combined with real-time quantitative PCR (qPCR) or digital PCR (dPCR) التقنيات, achieve highly sensitive detection of fungi.

مقدمة المورد

شركة شنغهاي لينغجون للتكنولوجيا الحيوية, المحدودة.تأسست في 2016 وهي شركة متخصصة في تصنيع المواد المغناطيسية الحيوية وكواشف استخلاص الحمض النووي.

لدينا خبرة غنية في استخراج وتنقية الحمض النووي, تنقية البروتين, فصل الخلايا, التألق الكيميائي والمجالات التقنية الأخرى.

منتجاتنا تستخدم على نطاق واسع في العديد من المجالات, مثل الفحوصات الطبية, الاختبارات الجينية, البحوث الجامعية, التكاثر الوراثي, وهكذا. نحن لا نقدم المنتجات فحسب، بل يمكننا أيضًا إجراء تصنيع المعدات الأصلية, أوديإم, وغيرها من الاحتياجات. إذا كان لديك الاحتياجات ذات الصلة, لا تتردد في الاتصال بنا على sales01@lingjunbio.com.