Manik Magnetik Lnjnbio untuk Kit Ekstraksi DNA Jamur 231018

(Metode Manik Magnetik)

【Pengenalan Produk】

Fungal mycelium, sporocarp, spores, and other samples release nucleic acid under the action of lysate. Asam nukleat dipisahkan dari pengotor lain dalam sampel dengan adsorpsi spesifik manik-manik magnetik, and then rinsed with washing buffer to remove proteins, polisakarida, inorganic salts, dll., and finally eluted to obtain purified nucleic acid. The obtained nucleic acid products can be used for PCR, DNA detection and analysis, hibridisasi molekuler, deteksi mutasi gen, dan pengurutan.

【Keunggulan produk】

1. Core raw materials are produced by ourselves and product quality and output are guaranteed.

2. The extraction products had high purity, hasil tinggi dan pita elektroforesis lengkap.

3. Ekstraksi metode manik magnetik dapat mewujudkan operasi otomatis.

4. The process of use is safe, and the product does not contain phenol, kloroform dan reagen berbahaya lainnya.

5. Because of strong versatiCore raw materials are produced by ourselves and product quality and output are guaranteed.

Kemurnian tinggi: There is no need to use toxic organic solvents such as phenol and chloroform during the extraction process. This avoids contamination from impurities and ensures high-purity DNA, which can be directly used for molecular biology experiments such as restriction enzyme digestion, PCR, dan pengurutan.

Pengoperasian yang mudah: The manual operation time is short, and it can be used in conjunction with liquid handling workstations or fully automated nucleic acid extraction instruments to achieve automated operation, making it suitable for high-throughput sample processing.

Keamanan tinggi: The extraction process is safe and environmentally friendly, with no involvement of hazardous reagents, protecting the health of laboratory personnel.

Fleksibilitas yang kuat: It is applicable to various fungal samples, including mycelium, fruiting bodies, spores, etc.lity, it is suitable for a variety of fungi mycelium, sporocarp, spores and other samples for the extraction of nucleic acids.

【Gambar produk】

【Kasus eksperimental】

Kasus 1:

For different fungal samples, DNA was extracted with the results as follows:

NHai.	Mencicipi	Sampe intake (mg)	A260/A280	A260/A230	Kesimpulan(ng/μL)
1	Pleurotus eryngii	20	1.84	1.63	346.6
2			1.82	1.68	344.0
3	Pleurotus ostreatus	10	1.82	1.86	278.6
4			1.87	1.91	242.2
5	White Shimeji	10	1.93	2.22	112.5
6			1.96	2.17	97.5

The electropherogram is as follows:

Analisis hasil: DNA from various fungal samples extracted with this product showed good purity, high yield and complete bands.

Kasus 2：

For different fungal samples, nucleic acids were extracted and the results were as follows:

NHai.	Mencicipi intake	A260/A280	A260/A230	Kesimpulan(dari/ul)	Menghasilkan(μg)
1	5 mg Yeast	1.948	2.095	95.8	9.58
2		1.953	2.113	100.1	10.01
Rata-rata		1.9505	2.104	97.95	9.795
3	5mg Aspergillus hyphae	1.863	1.863	176.9	17.69
4		1.862	1.868	174.35	17.435
Rata-rata		1.8625	1.8655	175.625	17.5625
5	20mg Lentinula edodes	1.923	2.142	321.55	32.155
6		1.921	2.139	324.05	32.405
Rata-rata		1.922	2.1405	322.8	32.28
7	30 mgHypsizygus marmoreus	1.94	1.993	619.35	61.935
8		1.944	1.985	626	62.6
Rata-rata		1.942	1.989	622.675	62.2675
9	30 mgEryngium campestre	1.972	2.181	141.55	14.155
10		1.968	2.194	141	14.1
Rata-rata		1.97	2.1875	141.275	14.1275

Diagram pola puncak:

Yeast:

Aspergillus hyphae:

Lentinula edodes:

Hypsizygusmarm oreus:

Eryngium campestre:

Diagram puncak total:

Elektroforogram ditunjukkan di bawah ini:

M:Penanda (2.000 bp, 1,000 bp, 750 bp, 500 bp, 250 bp, 100 bp）;

1-2: 5 mg Yeast             3-4: 5mgAspergillus hyphae        5-6: 20mgLentinula edodes  

7-8: 30 mgHypsizygus marmoreus                         9-10: 30 mgEryngium campestre

Analisis hasil:

Hasil uji Ultra-Micro Nucleic Acid Assay menunjukkan hal tersebut:

① The value of A₂₆₀ / A₂₈₀ is from 1.86 ke 1.97, and the value of A₂₆₀ / A₂₃₀ is above 1.86. The complete and basic coincidence of peak shape prove the high purity of the extraction product.

② The nucleic acid extraction yield of different fungal samples was above 9 mg.

The results of the agarose gel electrophoresis showed that:

① Different fungal samples have bright and good integrity bands.

② In conclusion, using automatic nucleic acid extraction and purification instrument to extract different fungal samples, the extraction product has high purity, hasil tinggi, complete electrophoretic band. The reagent has good stability when used with the instrument.

【Area aplikasi】

Molecular Biology Research

The extracted fungal DNA can be used in various molecular biology experiments, including genome sequencing, kloning gen, amplifikasi PCR, restriction enzyme digestion, and library construction. These experiments help researchers gain a deep understanding of the structure, function, and evolutionary relationships of fungal genomes.

• Microbial Identification and Classification

In the field of microbiology, the reagent can be used to rapidly extract fungal DNA for the identification and classification of different fungal species. Through gene sequencing or PCR technology, the types of fungi can be accurately identified, especially for studies on microbial diversity in complex environmental samples.

• Diagnosa Klinis

In clinical medicine, fungal DNA extraction reagents can be used to detect fungal infectious diseases. By extracting fungal DNA from patient samples and combining it with PCR or sequencing technologies, fungal infections can be diagnosed quickly and accurately, providing a basis for clinical treatment.

• Food Safety Testing

In the field of food safety, the reagent can be used to detect fungal contamination in food, such as molds and yeasts. By extracting fungal DNA from food samples, rapid detection of potential pathogenic fungi can be achieved, memastikan keamanan pangan.

• Environmental Microbial Detection

Dalam ilmu lingkungan, fungal DNA extraction reagents can be used to analyze the diversity of fungi in environmental samples. Misalnya, extracting fungal DNA from soil and water samples helps study the microbial composition and function of ecosystems.

• Forensic Identification

In the field of forensic science, the reagent can be used to extract fungal DNA from biological samples for microbial evidence analysis in cases. Misalnya, detecting specific fungi in environmental samples can provide supplementary evidence for investigations.

• Bioproducts and Cell Culture

In the fields of biotechnology and pharmaceuticals, fungal DNA extraction reagents can be used to detect fungal contamination in bioproducts (such as vaccines and cell therapy products), ensuring product quality and safety.

• High-Throughput Automation Applications

The magnetic bead-based extraction reagent has good compatibility with automation and can be used in combination with automated nucleic acid extraction instruments to achieve high-throughput sample processing. This is particularly advantageous for large-scale sample testing, improving efficiency and reducing human error.

• Gene Function Research

The extracted fungal DNA can be used for gene function studies, such as investigating the functions of fungal genes through techniques like gene knockout and overexpression.

1. Molecular Diagnostics and Nucleic Acid Testing

Di bidang diagnostik molekuler, the reagent can be used to extract fungal DNA from samples and, combined with real-time quantitative PCR (qPCR) or digital PCR (dPCR) teknologi, achieve highly sensitive detection of fungi.

Pengenalan Pemasok

Shanghai Lingjun Bioteknologi Co., Ltd.didirikan pada 2016 dan merupakan produsen profesional bahan biomagnetik dan reagen ekstraksi asam nukleat.

Kami memiliki pengalaman yang kaya dalam ekstraksi dan pemurnian asam nukleat, pemurnian protein, pemisahan sel, chemiluminescence dan bidang teknis lainnya.

Produk kami banyak digunakan di berbagai bidang, misalnya tes kesehatan, pengujian genetik, penelitian universitas, pemuliaan genetik, dan sebagainya. Kami tidak hanya menyediakan produk tetapi juga dapat melakukan OEM, ODM, dan kebutuhan lainnya. Jika Anda memiliki kebutuhan terkait, jangan ragu untuk menghubungi kami di sales01@lingjunbio.com.