Lnjnbio magnetiske perler til plantegenomisk DNA-ekstraktionssæt 231015

(Magnetisk perlemetode, No Centrifugation)

【Produktintroduktion】

This kit is mainly used to extract genomic DNA from a variety of plant tissues, the lysis buffer directly lyses plant samples to release nucleic acids, and the magnetic beads selectively adsorb the nucleic acids in the lysis buffer under the action of the binding buffer, remove a small amount of impurities adsorbed by the magnetic beads through the cleaning of the washing buffer, and release the adsorbed nucleic acids by the magnetic beads under the action of the elution buffer to obtain high-quality genomic DNA. The process is easy to operate and is especially suitable for automated nucleic acid extraction platforms. The extracted genomic DNA has high yield, høj renhed, stabil og pålidelig kvalitet, and can be applied to a variety of downstream molecular biology experiments.

【Produktfordele】

1. For both fresh and dried samples, excellent extraction results can be achieved;

2. The extracted product has high purity, højt udbytte, and complete electrophoresis bands;

3. It is highly versatile and suitable for extracting genomic DNA from leaves, frugter, seeds and other samples of different species of plants；

4. Brugsprocessen er sikker, no liquid nitrogen grinding is required, and the product does not contain harmful reagents such as phenol and chloroform.

【Produktbillede】

【Eksperimentel sag】

Sag 1:

Tabel 1、DNA extraction results from fresh Brassica rapa leaves

Ingen.	Prøve	A260/A280	A260/A230	Konc.(af/ul)
1	5mg Brassica rapa leaves A	1.859	1.745	280.6
2		1.854	1.68	401.35
Gns		1.857	1.713	340.98
3	5mg Brassica rapa leaves B	1.863	1.798	295.15
4		1.852	1.8	305.45
Gns		1.856	1.80	300.3
5	5mg Brassica rapa leaves C	1.925	1.863	461
6		1.907	1.843	527.7
Gns		1.916	1.853	494.35

Elektroferogrammet er vist nedenfor:

M:Markør (2K，1K，750bp，500bp，250bp，100bp)

1-2：5mg Brassica rapa leaves A 3-4：5mg Brassica rapa leaves B 5-6：5mg Brassica rapa leaves C

Resultatanalyse:

Resultaterne af Ultra-Micro Nucleic Acid Assay (UMNA) test viste det:

①The values of A260/A280 is above1.8 and A260/A230 is above 1.6, which proves that the extracted products are of high purity.

②Highly yields of nucleic acids extracted from Brassica rapa leaves.

Resultaterne af agarosegelelektroforese viste det:

①5 mg Brassica rapa leaves have bright bands and good integrity.

Som konklusion, the extracted genomic DNA of Brassica rapa leaves samples were of high purity, højt udbytte og komplette elektroforetiske bånd.

Sag 2:

Tabel 2、DNA extraction results from frozen tobacoo leaves

Prøve	Ingen.	A260/A280	A260/A230	Konc.(ng/μL)
frozen tobacoo leaves 5 mg	1	1.821	1.318	14.2
	2	1.831	1.311	15.4
	Gns	1.826	1.3145	14.8
	3	1.872	1.586	61.9
	4	1.803	1.421	63.5
	Gns	1.8375	1.5035	62.7
	5	1.828	1.436	24.3
	6	1.921	1.226	19.5
	Gns	1.8745	1.331	21.9

Elektroferogrammet er vist nedenfor:

Resultatanalyse: The DNA extracted from frozen tobacco leaves with this product had good purity, high yield and intact bands.

Sag 3:

Tabel 3、DNA extraction results from fresh pothos leaves

Ingen	Prøve	Prøveindtag (mg)	A260/A280	A260/A230	Konc.(ng/µL)
1	fresh pothos leaves	10 mg	1.862	1.197	47.75
2			1.885	1.052	50.95
3			1.829	0.987	59.4
4			1.854	1.028	56.45

Elektroferogrammet er vist nedenfor:

Resultatanalyse: The DNA extracted from fresh tobacco leaves with this product had good purity, high yield and intact bands.

【ENanvendelsesområde】

Genomic DNA purified by this kit can be used for PCR, qPCR, Enzym fordøjelse, SNP-genotypning , Next-generation sequencing, biblioteksbyggeri,molecular breeding and other biological experiments .

Vores produkter er meget udbredt inden for mange områder, såsom medicinsk test, genetisk testning, universitetsforskning, genetisk avl, og så videre. Vi leverer ikke kun produkter, men kan også påtage os OEM, ODM, og andre behov. Hvis du har relaterede behov, Du er velkommen til at kontakte os på sales01@lingjunbio.com.

Leverandørintroduktion

Shanghai Lingjun Biotechnology Co., Ltd.blev etableret i 2016 og er en professionel producent af biomagnetiske materialer og nukleinsyreekstraktionsreagenser.

Vi har stor erfaring med udvinding og oprensning af nukleinsyre, proteinoprensning, celleadskillelse, kemiluminescens og andre tekniske områder.

Vores produkter er meget udbredt inden for mange områder, såsom medicinsk test, genetisk testning, universitetsforskning, genetisk avl, og så videre. Vi leverer ikke kun produkter, men kan også påtage os OEM, ODM, og andre behov. Hvis du har relaterede behov, Du er velkommen til at kontakte os på sales01@lingjunbio.com.