Lnjnbio magnetiske perler til plante-DNA/RNA-ekstraktionssæt 231016

(Magnetisk perlemetode, No Centrifugation)

【Produktintroduktion】

Plant DNA/RNA extraction reagent is suitable for quickly and efficiently extracting DNA/RNA from plant tissue samples such as leaves, frugter, frø, osv., and the whole process is convenient to operate, especially suitable for automated nucleic acid extraction platform. The extraction process releases nucleic acids under the action of the lysis buffer; Micron magnetic beads selectively adsorb nucleic acids under the action of the binding buffer, separating the nucleic acids from other impurities in the sample; Then rinse with washing buffer to remove proteins, polysaccharider, uorganiske saltioner, osv.; Endelig, high-quality DNA/RNA is obtained after elution. This product does not require toxic reagents such as phenol and chloroform, and the extracted DNA/RNA has high yield, høj renhed, stabil og pålidelig kvalitet, and can be applied to a variety of downstream molecular biology experiments.

【Produktfordele】

1. Nem at betjene, magnetic bead method can be combined with automatic nucleic acid extractor to achieve automatic extraction, one-step lysis and extraction efficiently;

2. There is no need for repeated centrifugation to ensure the integrity of the extracted DNA/RNA;

3. The extracted product has good purity, high yield and complete electrophoresis band;

4. Brugsprocessen er sikker, no liquid nitrogen grinding is required, and the product does not contain harmful reagents such as phenol and chloroform;

5. It is highly versatile, suitable for extracting DNA/RNA from leaves, frugter, seeds and other samples of different types of plants.

【Produktbillede】

 

【Eksperimentel sag】

Sag 1:

Tabel 1、DNA/RNA extraction results from fresh Brassica rapa leaves

Ingen.	Prøve	A260/A280	A260/A230	Konc.(af/ul)
1	5mg Brassica rapa leaves A	1.973	1.971	775
2		1.944	1.925	809.6
Gns		1.9585	1.948	792.3
3	5mg Brassica rapa leaves B	1.972	2.017	743.15
4		1.922	1.816	754.1
Gns		1.947	1.9165	748.625
5	5mg Brassica rapa leaves C	2.007	2.221	711.05
6		1.942	2.087	331.25
Gns		1.974	2.154	521.15

Elektroferogrammet er vist nedenfor:

M:Markør (2K，1K，750bp，500bp，250bp，100bp)

1-2：5mg Brassica rapa leaves A 3-4：5mg Brassica rapa leaves B 5-6：5mg Brassica rapa leaves C

Resultatanalyse:

Resultaterne af Ultra-Micro Nucleic Acid Assay (UMNA) test viste det:

①The values of A260/A280 and A260/A230 are above 1.9, which proves that the extracted products are of high purity.

②Highly yields of nucleic acids extracted from Brassica rapa leaves.

Resultaterne af agarosegelelektroforese viste det:

①All the samples of different plant leaves have bright bands and good integrity.

Som konklusion, de ekstraherede produkter af forskellige plantebladsprøver var af høj renhed, højt udbytte og komplette elektroforetiske bånd.

Sag 2:

Tabel 2、DNA/RNA extraction results from fresh tobacoo leaves

Prøve	Ingen.	A260/A280	A260/A230	Konc.(ng/μL)
Fresh tobacoo leaves 10 mg	1	2.08	1.527	90.55
	2	2.075	1.52	89.85
	3	2.045	1.397	98.05
	4	2.029	1.475	102.95

Elektroferogrammet er vist nedenfor:

Resultatanalyse: The DNA/RNA extracted from fresh tobacco leaves with this product had good purity, high yield and intact bands.

Sag 3:

Tabel 3、DNA/RNA extraction results from fresh pothos leaves

样本	Ingen.	A260/A280	A260/A230	Konc.(ng/μL)
fresh pothos leaves 50mg	1	2.078	1.511	85.2
	2	2.1	1.426	99.5
	3	2.094	1.104	92.3
	4	2.1	1.499	78.5
	5	2.087	1.579	93.85

Elektroferogrammet er vist nedenfor:

Resultatanalyse: The DNA/RNA extracted from fresh tobacco leaves with this product had good purity, high yield and intact bands.

【ENanvendelsesområde】

Nucleic acids purified by this kit can be used for PCR, qPCR, Enzym fordøjelse, library construction and Southern-Blot.

Vores produkter er meget udbredt inden for mange områder, såsom medicinsk test, genetisk testning, universitetsforskning, genetisk avl, og så videre. Vi leverer ikke kun produkter, men kan også påtage os OEM, ODM, og andre behov. Hvis du har relaterede behov, Du er velkommen til at kontakte os på sales01@lingjunbio.com.

Leverandørintroduktion

Shanghai Lingjun Biotechnology Co., Ltd.blev etableret i 2016 og er en professionel producent af biomagnetiske materialer og nukleinsyreekstraktionsreagenser.

Vi har stor erfaring med udvinding og oprensning af nukleinsyre, proteinoprensning, celleadskillelse, kemiluminescens og andre tekniske områder.

Vores produkter er meget udbredt inden for mange områder, såsom medicinsk test, genetisk testning, universitetsforskning, genetisk avl, og så videre. Vi leverer ikke kun produkter, men kan også påtage os OEM, ODM, og andre behov. Hvis du har relaterede behov, Du er velkommen til at kontakte os på sales01@lingjunbio.com.