Lnjnbio magnetiske perler til animalsk vævs-DNA/RNA-ekstraktionssæt 231012

(Magnetisk perlemetode)

【Produktintroduktion】

This kit uses magnetic beads and buffer system with unique separation effect to extract DNA/RNA from animal tissues, the whole process is easy to operate especially suitable for automated nucleic acid extraction platform. The extracted nucleic acids are of high yield, høj renhed, stabil og pålidelig kvalitet, og kan anvendes til forskellige nedstrøms molekylærbiologiske eksperimenter.

【Produktfordele】

1, Simple and fast: direct lysis, no need to incubate for long time, high quality DNA/RNA can be obtained within 30 min

2, Safe process: no liquid nitrogen grinding of samples

3, Sikker og ikke-giftig: no organic reagents such as phenol/chloroform required

4, Bred vifte af applikationer: applicable to a wide range of animal tissues, successfully extracted from pork, beef, fish, shrimp, porcine and other samples to obtain DNA / RNA

5, Høj gennemstrømning: can be used with automated nucleic acid extraction platforms to realize high-throughput extraction

【Produktbillede】

【Eksperimentel sag】

Sag: Different tissue samples from different animals were extracted for DNA/RNA and the results were as follows:

Antal	Prøve	A260/A280	A260/A230	Konc.(ng/μL)	Udbytte(μg)	CV
1	20mgFish	1.856	1.884	109.25	10.925
2		1.866	1.956	117.4	11.74
Gennemsnit		1.861	1.92	113.325	11.3325	0.28%
3	20mg Chicken meat	1.886	2.046	284.55	28.455
4		1.889	2.041	249.4	24.94
Gennemsnit		1.8875	2.0435	266.975	26.6975	6.58%
5	5mg fiskelever	1.806	1.587	256.45	25.645
6		1.806	1.572	253.4	25.34
Gennemsnit		1.806	1.5795	254.925	25.4925	0.60%
7	5mg Kyllingehjerte	1.856	1.966	101.95	10.195
8		1.85	1.999	107.2	10.72
Gennemsnit		1.853	1.9825	104.575	10.4575	2.51%

Peak mønster diagram:

Fish：

Chicken meat:

Fish liver：

Chicken heart：

Total peak shape：

Elektroferogrammet er vist nedenfor:

Resultatanalyse:

Resultaterne af Ultra-Micro Nucleic Acid Assay (UMNA) test viste det:

①A260/A280, A260/A230, the coefficient of variation was below 6.58%, which proved the high stability of reagent extraction.

②The values of A260/A280 were above 1.8, and the values of A260/A230 were in the range of 1.5-2.0, and the peak shapes were complete and basically overlapped proving that the extracted products were of high purity.

③Nucleic acid extraction yields ranged from 10-28 µg or more for different animal tissue samples.

Resultaterne af agarosegelelektroforese viste det:

①Different tissue samples from different animals have bright bands with good integrity.

Som konklusion: the nucleic acids of different animal samples were extracted by the fully automatic nucleic acid extraction and purification instrument, and the extracted products were of high purity, højt udbytte, complete electrophoretic bands, and the reagents were uniform and stable in cooperation with the instrument.

【ENanvendelsesområde】

Nucleic acids purified by this kit can be used for PCR, qPCR, digestion, library construction and Southern blot.

Leverandørintroduktion

Shanghai Lingjun Biotechnology Co., Ltd.blev etableret i 2016 og er en professionel producent af biomagnetiske materialer og nukleinsyreekstraktionsreagenser.

Vi har stor erfaring med udvinding og oprensning af nukleinsyre, proteinoprensning, celleadskillelse, kemiluminescens og andre tekniske områder.

Vores produkter er meget udbredt inden for mange områder, såsom medicinsk test, genetisk testning, universitetsforskning, genetisk avl, og så videre. Vi leverer ikke kun produkter, men kan også påtage os OEM, ODM, og andre behov. Hvis du har relaterede behov, Du er velkommen til at kontakte os på sales01@lingjunbio.com.