Manik Magnetik Lnjnbio untuk Kit Ekstraksi DNA/RNA Jaringan Hewan 231012

(Metode Manik Magnetik)

【Pengenalan Produk】

This kit uses magnetic beads and buffer system with unique separation effect to extract DNA/RNA from animal tissueS, the whole process is easy to operate especially suitable for automated nucleic acid extraction platform. The extracted nucleic acids are of high yield, kemurnian tinggi, kualitas stabil dan dapat diandalkan, and can be applied to various downstream molecular biology experiments.

【Keunggulan produk】

1, Simple and fast: direct lysis, no need to incubate for long time, high quality DNA/RNA can be obtained within 30 menit

2, Safe process: no liquid nitrogen grinding of samples

3, Aman dan tidak beracun: no organic reagents such as phenol/chloroform required

4, Berbagai macam aplikasi: applicable to a wide range of animal tissues, successfully extracted from pork, beef, fish, shrimp, porcine and other samples to obtain DNA / RNA

5, High-throughput: can be used with automated nucleic acid extraction platforms to realize high-throughput extraction

【Gambar produk】

【Kasus eksperimental】

Kasus: Different tissue samples from different animals were extracted for DNA/RNA and the results were as follows:

Nomor	Mencicipi	A260/A280	A260/A230	Kesimpulan(ng/μL)	Menghasilkan(μg)	CV
1	20mgFish	1.856	1.884	109.25	10.925
2		1.866	1.956	117.4	11.74
Rata-rata		1.861	1.92	113.325	11.3325	0.28%
3	20mg Chicken meat	1.886	2.046	284.55	28.455
4		1.889	2.041	249.4	24.94
Rata-rata		1.8875	2.0435	266.975	26.6975	6.58%
5	5mg hati ikan	1.806	1.587	256.45	25.645
6		1.806	1.572	253.4	25.34
Rata-rata		1.806	1.5795	254.925	25.4925	0.60%
7	5mg Hati ayam	1.856	1.966	101.95	10.195
8		1.85	1.999	107.2	10.72
Rata-rata		1.853	1.9825	104.575	10.4575	2.51%

Diagram pola puncak:

Fish：

Chicken meat:

Fish liver：

Chicken heart：

Total peak shape：

Elektroforogram ditunjukkan di bawah ini:

Analisis hasil:

Hasil Uji Asam Nukleat Ultra Mikro (UMNA) tes menunjukkan hal itu:

①A260/A280, A260/A230, the coefficient of variation was below 6.58%, which proved the high stability of reagent extraction.

②The values of A260/A280 were above 1.8, and the values of A260/A230 were in the range of 1.5-2.0, and the peak shapes were complete and basically overlapped proving that the extracted products were of high purity.

③Nucleic acid extraction yields ranged from 10-28 µg or more for different animal tissue samples.

Hasil elektroforesis gel agarosa menunjukkan bahwa:

①Different tissue samples from different animals have bright bands with good integrity.

Kesimpulannya: the nucleic acids of different animal samples were extracted by the fully automatic nucleic acid extraction and purification instrument, and the extracted products were of high purity, hasil tinggi, complete electrophoretic bands, and the reagents were uniform and stable in cooperation with the instrument.

【Aarea aplikasi】

Asam nukleat yang dimurnikan dengan kit ini dapat digunakan untuk PCR, qPCR, digestion, library construction and Southern blot.

Pengenalan Pemasok

Shanghai Lingjun Bioteknologi Co., Ltd.didirikan pada 2016 dan merupakan produsen profesional bahan biomagnetik dan reagen ekstraksi asam nukleat.

Kami memiliki pengalaman yang kaya dalam ekstraksi dan pemurnian asam nukleat, pemurnian protein, pemisahan sel, chemiluminescence dan bidang teknis lainnya.

Produk kami banyak digunakan di berbagai bidang, misalnya tes kesehatan, pengujian genetik, penelitian universitas, pemuliaan genetik, dan sebagainya. Kami tidak hanya menyediakan produk tetapi juga dapat melakukan OEM, ODM, dan kebutuhan lainnya. Jika Anda memiliki kebutuhan terkait, jangan ragu untuk menghubungi kami di sales01@lingjunbio.com.