Lnjnbio Magnéitesch Perlen fir Déieregewebe DNA / RNA Extraktioun Kit 231012

(Magnéitesch Bead Method)

【Produktintroduktioun】

This kit uses magnetic beads and buffer system with unique separation effect to extract DNA/RNA from animal tissues, the whole process is easy to operate especially suitable for automated nucleic acid extraction platform. The extracted nucleic acids are of high yield, héich Rengheet, stabil an zouverlässeg Qualitéit, and can be applied to various downstream molecular biology experiments.

【Produktvirdeeler】

1, Simple and fast: direct lysis, no need to incubate for long time, high quality DNA/RNA can be obtained within 30 min

2, Safe process: no liquid nitrogen grinding of samples

3, Sécher an net gëfteg: no organic reagents such as phenol/chloroform required

4, Breet Palette vun Uwendungen: applicable to a wide range of animal tissues, successfully extracted from pork, beef, fish, shrimp, porcine and other samples to obtain DNA / RNA

5, High-throughput: can be used with automated nucleic acid extraction platforms to realize high-throughput extraction

【Produktbild】

【Experimentell Fall】

Fall: Different tissue samples from different animals were extracted for DNA/RNA and the results were as follows:

Zuel	Prouf	A260/A280	A260/A230	Konz.(ng/μL)	Rendement(μg)	CV
1	20mg Fësch	1.856	1.884	109.25	10.925
2		1.866	1.956	117.4	11.74
Duerchschnëtt		1.861	1.92	113.325	11.3325	0.28%
3	20mg Poulet Fleesch	1.886	2.046	284.55	28.455
4		1.889	2.041	249.4	24.94
Duerchschnëtt		1.8875	2.0435	266.975	26.6975	6.58%
5	5mg Fësch Liewer	1.806	1.587	256.45	25.645
6		1.806	1.572	253.4	25.34
Duerchschnëtt		1.806	1.5795	254.925	25.4925	0.60%
7	5mg Poulet Häerz	1.856	1.966	101.95	10.195
8		1.85	1.999	107.2	10.72
Duerchschnëtt		1.853	1.9825	104.575	10.4575	2.51%

Peak Muster Diagramm:

Fësch：

Poulet Fleesch:

Fësch Liewer：

Poulet Häerz：

Total peak shape：

Den Elektropherogramm gëtt hei ënnen gewisen:

Resultat Analyse:

D'Resultater vum Ultra-Micro Nucleic Acid Assay (UMNA) Test huet dat gewisen:

①A260/A280, A260/A230, de Koeffizient vun der Variatioun war ënnert 6.58%, wat déi héich Stabilitéit vun der Reagensextraktioun bewisen huet.

②The values of A260/A280 were above 1.8, and the values of A260/A230 were in the range of 1.5-2.0, an d'Spëtzeforme ware komplett a grondsätzlech iwwerlappt, beweist datt d'extraktéiert Produkter vu héijer Rengheet waren.

③Nukleinsäure Extraktioun Erléisse gounge vun 10-28 µg oder méi fir verschidden Déieregewebe Proben.

D'Resultater vun der Agarosegel Elektrophorese weisen dat:

①Different tissue samples from different animals have bright bands with good integrity.

Als Conclusioun: the nucleic acids of different animal samples were extracted by the fully automatic nucleic acid extraction and purification instrument, and the extracted products were of high purity, héich nozeginn, komplett elektrophoretic Bands, and the reagents were uniform and stable in cooperation with the instrument.

【AUwendungsberäich】

Nukleinsäuren, déi vun dësem Kit gereinegt ginn, kënne fir PCR benotzt ginn, qPCR, digestion, Bibliothéik Bau an Southern blot.

Fournisseur Aféierung

Shanghai Lingjun Biotechnology Co., Ltd., Ltd.gouf etabléiert an 2016 an ass e professionnelle Hiersteller vu biomagnetesche Materialien an Nukleinsäure Extraktiounsreagenz.

Mir hu räich Erfahrung an Nukleinsäure Extraktioun an Offäll, Protein Reinigung, Zell Trennung, Chemilumineszenz an aner technesch Felder.

Eis Produkter gi wäit a ville Beräicher benotzt, wéi medizinesch Tester, genetesch Tester, Universitéit Fuerschung, genetesch Zucht, an esou weider. Mir liwweren net nëmmen Produkter awer kënnen och OEM maachen, ODM, an aner Besoinen. Wann Dir verbonne Besoinen hutt, w.e.g. kontaktéiert eis op sales01@lingjunbio.com.