Lnjnbio Magnéitesch Perlen fir Bakterien DNA / RNA Extraktioun Kit 231017

(Magnéitesch Bead Method)

【Produktintroduktioun】

This product uses a lysis buffer to directly lyse Gram-negative samples or lyse Gram-positive samples with lysozyme to release nucleic acids, and the magnetic beads selectively adsorb nucleic acids in the lysis buffer under the action of the binding buffer. A small amount of impurities adsorbed by the magnetic beads are removed by cleaning the washing buffer. Under the action of the elution buffer, the magnetic beads release the adsorbed nucleic acid, namely to obtain high-quality DNA / RNA. The DNA / RNA extracted from this product requires no toxic reagents such as phenol and chloroform, an den obtained DNA / RNA can be directly used for downstream experiments.

【Produktvirdeeler】

1. Before the magnetic bead method to extraction, the bacterial DNA / RNA with high purity and better integrity can be obtained without centrifugation.
2. Simple and fast, 10-30 µg of nucleic acid can be obtained from 1 mL of bacterial buffer for about 30 min.

3. Magnetic bead method extraction can process multiple samples at the same time, achieving high throughput and automation extraction.

4.The use process is safe, ouni flësseg Stickstoff Schleifen, d'Produkt enthält keng Phenol, chloroform, an aner schiedlech reagents.

5. Proteinase K does not need to be added in the extraction proces. The kit can be transported and stored at room temperature.

6. Héich Sensibilitéit, the kit can be suitable for trace sample extraction.

【Produktbild】

【Experimentell Fäll】

Fall 1: E. coli was grown overnight, the bacteria were collected by centrifugation, and DNA / RNA was extracted. The results were as follows:

Zuel	Prouf	A260/A280	A260/A230	Konz.(ng/μL)	Rendement(μg)
1	500 μL	1.952	2.084	57.45	5.745
2		1.956	2.052	57.35	5.735
Avg		1.954	2.068	57.4	5.74
3	600 μL	1.939	2.017	79.45	7.945
4		1.934	2.028	79.45	7.675
Avg		1.937	2.023	78.1	7.81
5	800 μL	1.953	2.110	98.6	9.86
6		1.956	2.111	96.75	9.675
Avg		1.955	2.111	97.675	9.7675

Peak Muster Diagramm:

Bacterial solution of 500 μL:

Bacterial solution of 600 μL:

Bacterial solution of 800 μL:

Total Peak Diagramm:

Den Elektropherogramm gëtt hei ënnen gewisen:

M:Marker（2,000 bp, 1,000 bp, 750 bp, 500 bp, 250 bp, 100 bp);

1-2:Bacterial solution of 500 μL

3-4:Bacterial solution of 600 μL

5-6:Bacterial solution of 800 μL

Resultat Analyse:

D'Resultater vum Ultra-Micro Nucleic Acid Assay Test hunn dat gewisen:

① The value of A₂₆₀ / A₂₈₀ is above 1.9, and the value of A₂₆₀ / A₂₃₀ is above 2.0. The complete and basic coincidence of peak shape prove the high purity of the extraction product.

② The nucleic acid extraction yield of different E.coli samples was above 5 µg.

The results of the agarose gel electrophoresis showed that:

① Different E.coli samples have bright and good integrity bands.

Als Conclusioun, the nucleic acid extraction yield of E.coli has high purity, héich nozeginn, complete electrophoretic band.

【Uwendungsberäich】

The purified nucleic acids extracted from this kit can be used for PCR, qPCR, Enzym Verdauung, Bibliothéik Bau, and Southern-Blot.

Eis Produkter gi wäit a ville Beräicher benotzt, wéi medizinesch Tester, genetesch Tester, Universitéit Fuerschung, genetesch Zucht, an esou weider. Mir liwweren net nëmmen Produkter awer kënnen och OEM maachen, ODM, an aner Besoinen. Wann Dir verbonne Besoinen hutt, w.e.g. kontaktéiert eis op sales01@lingjunbio.com.

Fournisseur Aféierung

Shanghai Lingjun Biotechnology Co., Ltd., Ltd.gouf etabléiert an 2016 an ass e professionnelle Hiersteller vu biomagnetesche Materialien an Nukleinsäure Extraktiounsreagenz.

Mir hu räich Erfahrung an Nukleinsäure Extraktioun an Offäll, Protein Reinigung, Zell Trennung, Chemilumineszenz an aner technesch Felder.

Eis Produkter gi wäit a ville Beräicher benotzt, wéi medizinesch Tester, genetesch Tester, Universitéit Fuerschung, genetesch Zucht, an esou weider. Mir liwweren net nëmmen Produkter awer kënnen och OEM maachen, ODM, an aner Besoinen. Wann Dir verbonne Besoinen hutt, w.e.g. kontaktéiert eis op sales01@lingjunbio.com.