Lnjnbio Coirníní Maighnéadacha le haghaidh Baictéar DNA / Trealamh Eastósctha RNA 231017

(Modh Coirnín Maighnéadach)

【Réamhrá Táirge】

This product uses a lysis buffer to directly lyse Gram-negative samples or lyse Gram-positive samples with lysozyme to release nucleic acids, and the magnetic beads selectively adsorb nucleic acids in the lysis buffer under the action of the binding buffer. A small amount of impurities adsorbed by the magnetic beads are removed by cleaning the washing buffer. Under the action of the elution buffer, the magnetic beads release the adsorbed nucleic acid, namely to obtain high-quality DNA / RNA. The DNA / RNA extracted from this product requires no toxic reagents such as phenol and chloroform, agus an obtained DNA / RNA can be directly used for downstream experiments.

【Buntáistí táirge】

1. Before the magnetic bead method to extraction, the bacterial DNA / RNA with high purity and better integrity can be obtained without centrifugation.
2. Simplí agus tapa, 10-30 µg of nucleic acid can be obtained from 1 mL of bacterial buffer for about 30 nóim.

3. Magnetic bead method extraction can process multiple samples at the same time, achieving high throughput and automation extraction.

4.Tá an próiseas úsáide sábháilte, gan meilt nítrigine leachtach, níl feanól sa táirge, clóraform, and other harmful reagents.

5. Proteinase K does not need to be added in the extraction proces. The kit can be transported and stored at room temperature.

6. Íogaireacht ard, the kit can be suitable for trace sample extraction.

【Íomhá táirge】

【Cásanna turgnamhacha】

Cás 1: E. coli was grown overnight, the bacteria were collected by centrifugation, agus DNA / RNA was extracted. The results were as follows:

Uimhir	Sampla	A260/A280	A260/A230	Conc.(ng/μL)	Toradh(μg)
1	500 μL	1.952	2.084	57.45	5.745
2		1.956	2.052	57.35	5.735
Meán		1.954	2.068	57.4	5.74
3	600 μL	1.939	2.017	79.45	7.945
4		1.934	2.028	79.45	7.675
Meán		1.937	2.023	78.1	7.81
5	800 μL	1.953	2.110	98.6	9.86
6		1.956	2.111	96.75	9.675
Meán		1.955	2.111	97.675	9.7675

Léaráid patrún buaic:

Bacterial solution of 500 μL:

Bacterial solution of 600 μL:

Bacterial solution of 800 μL:

Léaráid bhuaic iomlán:

Taispeántar an electropherogram thíos:

M:Marcóir (2,000 bp, 1,000 bp, 750 bp, 500 bp, 250 bp, 100 bp）;

1-2:Bacterial solution of 500 μL

3-4:Bacterial solution of 600 μL

5-6:Bacterial solution of 800 μL

Anailís ar thorthaí:

Léirigh torthaí na tástála Ultra-Micri-Aigéad Núicléach é sin:

① The value of A₂₆₀ / A₂₈₀ is above 1.9, agus luach A₂₆₀ / A₂₃₀ atá thuas 2.0. Cruthaíonn comhtharlú iomlán agus bunúsach cruth buaic íonacht ard an táirge eastósctha.

② The nucleic acid extraction yield of different E.coli samples was above 5 µg.

Léirigh torthaí an leictreafóiréis glóthach agarose go:

① Different E.coli samples have bright and good integrity bands.

Mar fhocal scoir, the nucleic acid extraction yield of E.coli has high purity, toradh ard, complete electrophoretic band.

【Limistéar iarratais】

The purified nucleic acids extracted from this kit can be used for PCR, qPCR, díleá einsímí, tógáil leabharlainne, and Southern-Blot.

Úsáidtear ár gcuid táirgí go forleathan i go leor réimsí, mar thástáil leighis, tástáil ghéiniteach, taighde ollscoile, pórú géiniteach, agus mar sin de. Ní hamháin go gcuirimid táirgí ar fáil ach is féidir linn OEM a dhéanamh freisin, ODM, agus riachtanais eile. Má tá riachtanais ghaolmhara agat, bíodh leisce ort teagmháil a dhéanamh linn ag sales01@lingjunbio.com.

Réamhrá Soláthraí

Shanghai Lingjun Biteicneolaíocht Co., Teo.bunaíodh i 2016 agus is monaróir gairmiúil ábhair bhithmhaighnéadacha agus imoibrithe eastóscadh aigéid núicléacha.

Tá taithí shaibhir againn in eastóscadh agus íonú aigéad núicléasach, íonú próitéine, scaradh cille, chemiluminescence agus réimsí teicniúla eile.

Úsáidtear ár gcuid táirgí go forleathan i go leor réimsí, mar thástáil leighis, tástáil ghéiniteach, taighde ollscoile, pórú géiniteach, agus mar sin de. Ní hamháin go gcuirimid táirgí ar fáil ach is féidir linn OEM a dhéanamh freisin, ODM, agus riachtanais eile. Má tá riachtanais ghaolmhara agat, bíodh leisce ort teagmháil a dhéanamh linn ag sales01@lingjunbio.com.