Lnjnbio Magnetic Beads for Bacteria DNA/RNA Extraction Kit 231017

(마그네틱 비드 방식)

【제품소개】

This product uses a lysis buffer to directly lyse Gram-negative samples or lyse Gram-positive samples with lysozyme to release nucleic acids, and the magnetic beads selectively adsorb nucleic acids in the lysis buffer under the action of the binding buffer. A small amount of impurities adsorbed by the magnetic beads are removed by cleaning the washing buffer. Under the action of the elution buffer, the magnetic beads release the adsorbed nucleic acid, namely to obtain high-quality DNA / RNA. The DNA / RNA extracted from this product requires no toxic reagents such as phenol and chloroform, 그리고 obtained DNA / RNA can be directly used for downstream experiments.

【제품의 장점】

1. Before the magnetic bead method to extraction, the bacterial DNA / RNA with high purity and better integrity can be obtained without centrifugation.
2. Simple and fast, 10-30 µg of nucleic acid can be obtained from 1 mL of bacterial buffer for about 30 min.

3. Magnetic bead method extraction can process multiple samples at the same time, achieving high throughput and automation extraction.

4.The use process is safe, without liquid nitrogen grinding, the product does not contain phenol, chloroform, and other harmful reagents.

5. Proteinase K does not need to be added in the extraction proces. The kit can be transported and stored at room temperature.

6. High sensitivity, the kit can be suitable for trace sample extraction.

【상품 이미지】

【실험사례】

사례 1: E. coli was grown overnight, the bacteria were collected by centrifugation, and DNA / RNA was extracted. The results were as follows:

Number	견본	A260/A280	A260/A230	Conc.(ng/μL)	Yield(μg)
1	500 μL	1.952	2.084	57.45	5.745
2		1.956	2.052	57.35	5.735
Avg		1.954	2.068	57.4	5.74
3	600 μL	1.939	2.017	79.45	7.945
4		1.934	2.028	79.45	7.675
Avg		1.937	2.023	78.1	7.81
5	800 μL	1.953	2.110	98.6	9.86
6		1.956	2.111	96.75	9.675
Avg		1.955	2.111	97.675	9.7675

Peak pattern diagram:

Bacterial solution of 500 μL:

Bacterial solution of 600 μL:

Bacterial solution of 800 μL:

Total peak diagram:

전기영동도는 아래와 같습니다.:

M:Marker（2,000 bp, 1,000 bp, 750 bp, 500 bp, 250 bp, 100 bp）;

1-2:Bacterial solution of 500 μL

3-4:Bacterial solution of 600 μL

5-6:Bacterial solution of 800 μL

결과 분석:

The results of the Ultra-Micro Nucleic Acid Assay test showed that:

① The value of A₂₆₀ / 에이₂₈₀ is above 1.9, and the value of A₂₆₀ / 에이₂₃₀ is above 2.0. The complete and basic coincidence of peak shape prove the high purity of the extraction product.

② The nucleic acid extraction yield of different E.coli samples was above 5 µg.

The results of the agarose gel electrophoresis showed that:

① Different E.coli samples have bright and good integrity bands.

In conclusion, the nucleic acid extraction yield of E.coli has high purity, high yield, complete electrophoretic band.

【적용분야】

The purified nucleic acids extracted from this kit can be used for PCR, qPCR, enzyme digestion, library construction, and Southern-Blot.

우리의 제품은 많은 분야에서 널리 사용됩니다., 의료 테스트와 같은, 유전자 검사, 대학 연구, 유전자 육종, 등. 우리는 제품을 제공할 뿐만 아니라 OEM도 수행할 수 있습니다., ODM, 그리고 다른 필요. 관련 요구사항이 있는 경우, 언제든지 sales01@lingjunbio.com으로 문의해 주세요..

공급업체 소개

상하이 링쥔 생명공학 유한회사, 주식회사에 설립되었습니다 2016 생체자성재료 및 핵산추출시약 전문제조업체입니다..

핵산 추출 및 정제 분야에서 풍부한 경험을 보유하고 있습니다., 단백질 정제, 세포 분리, 화학발광 및 기타 기술 분야.

우리의 제품은 많은 분야에서 널리 사용됩니다., 의료 테스트와 같은, 유전자 검사, 대학 연구, 유전자 육종, 등. 우리는 제품을 제공할 뿐만 아니라 OEM도 수행할 수 있습니다., ODM, 그리고 다른 필요. 관련 요구사항이 있는 경우, 언제든지 sales01@lingjunbio.com으로 문의해 주세요..