PCR 증폭 키트(맞춤형)

Suitable for teaching molecular biology experiments in life sciences, 약, and agricultural and forestry disciplines, it serves as an ideal teaching tool for fundamental experiments such as gene cloning, genetic identification, 병원체 검출.

【제품소개】

This 2× Taq PCR Master Mix (with Dye) contains a highly efficient thermostable DNA polymerase, dNTPs, an optimised MgCl₂ buffer system, and bromophenol blue dye. This formulation maximises operational simplicity, minimises preparation errors, ensures reproducible results and high success rates, and requires only the addition of nucleic acid dye for electrophoresis following amplification.

【제품의 장점】

• User-friendly operation: Optimised premix components significantly streamline experimental procedures and lower operational barriers.
• Reliable results: Specifically optimised for teaching applications, delivering high amplification success rates, excellent reproducibility, and robust interference resistance.
• Customised versions allow selection of corresponding primers for amplifying transgenic plants (rice, maize, 등.) according to teaching and experimental requirements.

【실험 사례】

PCR amplification was performed using extracted rice seed genomic DNA as the template. The target product, SPS (sucrose phosphate synthase gene), has a size of 287 bp. The amplification reaction consisted of a 50 μL system with 200 ng template DNA and a primer annealing temperature of 60°C. Following amplification, 3 μL of the product was subjected to nucleic acid electrophoresis using DL 2000 as the marker. under conditions of 120 V에 대한 35 분. 전기영동 결과는 다음과 같습니다:

【적용분야】

This product is suitable for teaching molecular biology experiments in life sciences, 약, and agricultural and forestry disciplines. It can be used for fundamental experiments such as gene cloning, genetic identification, 병원체 검출, making it an ideal teaching tool.