Kit de amplificación por PCR (personalizado)

Suitable for teaching molecular biology experiments in life sciences, medicine, and agricultural and forestry disciplines, it serves as an ideal teaching tool for fundamental experiments such as gene cloning, genetic identification, y detección de patógenos.

【Introducción del producto】

This 2× Taq PCR Master Mix (with Dye) contains a highly efficient thermostable DNA polymerase, dNTPs, an optimised MgCl₂ buffer system, and bromophenol blue dye. This formulation maximises operational simplicity, minimises preparation errors, ensures reproducible results and high success rates, and requires only the addition of nucleic acid dye for electrophoresis following amplification.

【Ventajas del producto】

• User-friendly operation: Optimised premix components significantly streamline experimental procedures and lower operational barriers.
• Reliable results: Specifically optimised for teaching applications, delivering high amplification success rates, excellent reproducibility, and robust interference resistance.
• Customised versions allow selection of corresponding primers for amplifying transgenic plants (rice, maize, etc.) according to teaching and experimental requirements.

【Caso experimental】

PCR amplification was performed using extracted rice seed genomic DNA as the template. The target product, SPS (sucrose phosphate synthase gene), has a size of 287 pb. The amplification reaction consisted of a 50 μL system with 200 ng template DNA and a primer annealing temperature of 60°C. Following amplification, 3 μL of the product was subjected to nucleic acid electrophoresis using DL 2000 as the marker. under conditions of 120 V para 35 minutos. Los resultados de la electroforesis son los siguientes.:

【Área de aplicación】

This product is suitable for teaching molecular biology experiments in life sciences, medicine, and agricultural and forestry disciplines. It can be used for fundamental experiments such as gene cloning, genetic identification, y detección de patógenos, making it an ideal teaching tool.