Lnjnbio 동물 조직 DNA/RNA 추출 키트용 마그네틱 비드 231012

(마그네틱 비드 방식)

【제품소개】

This kit uses magnetic beads and buffer system with unique separation effect to extract DNA/RNA from animal tissue에스, the whole process is easy to operate especially suitable for automated nucleic acid extraction platform. The extracted nucleic acids are of high yield, high purity, stable and reliable quality, and can be applied to various downstream molecular biology experiments.

【제품의 장점】

1, Simple and fast: direct lysis, no need to incubate for long time, high quality DNA/RNA can be obtained within 30 min

2, Safe process: no liquid nitrogen grinding of samples

3, Safe and non-toxic: no organic reagents such as phenol/chloroform required

4, Wide range of applications: applicable to a wide range of animal tissues, successfully extracted from pork, beef, fish, shrimp, porcine and other samples to obtain DNA / RNA

5, High-throughput: can be used with automated nucleic acid extraction platforms to realize high-throughput extraction

【상품 이미지】

【Experimental case】

사례: Different tissue samples from different animals were extracted for DNA/RNA and the results were as follows:

Number	견본	A260/A280	A260/A230	Conc.(ng/μ엘)	Yield(μg)	CV
1	20mgFish	1.856	1.884	109.25	10.925
2		1.866	1.956	117.4	11.74
Average		1.861	1.92	113.325	11.3325	0.28%
3	20mg Chicken meat	1.886	2.046	284.55	28.455
4		1.889	2.041	249.4	24.94
Average		1.8875	2.0435	266.975	26.6975	6.58%
5	5mg Fish liver	1.806	1.587	256.45	25.645
6		1.806	1.572	253.4	25.34
Average		1.806	1.5795	254.925	25.4925	0.60%
7	5mg Chicken heart	1.856	1.966	101.95	10.195
8		1.85	1.999	107.2	10.72
Average		1.853	1.9825	104.575	10.4575	2.51%

Peak pattern diagram:

Fish：

Chicken meat:

Fish liver：

Chicken heart：

Total peak shape：

전기영동도는 아래와 같습니다.:

결과 분석:

The results of the Ultra-Micro Nucleic Acid Assay (UMNA) test showed that:

①A260/A280, A260/A230, the coefficient of variation was below 6.58%, which proved the high stability of reagent extraction.

②The values of A260/A280 were above 1.8, and the values of A260/A230 were in the range of 1.5-2.0, and the peak shapes were complete and basically overlapped proving that the extracted products were of high purity.

③Nucleic acid extraction yields ranged from 10-28 µg or more for different animal tissue samples.

The results of agarose gel electrophoresis showed that:

①Different tissue samples from different animals have bright bands with good integrity.

In conclusion: the nucleic acids of different animal samples were extracted by the fully automatic nucleic acid extraction and purification instrument, and the extracted products were of high purity, high yield, complete electrophoretic bands, and the reagents were uniform and stable in cooperation with the instrument.

【에이pplication area】

Nucleic acids purified by this kit can be used for PCR, qPCR, digestion, library construction and Southern blot.

공급업체 소개

상하이 링쥔 생명공학 유한회사, 주식회사에 설립되었습니다 2016 생체자성재료 및 핵산추출시약 전문제조업체입니다..

핵산 추출 및 정제 분야에서 풍부한 경험을 보유하고 있습니다., 단백질 정제, 세포 분리, 화학발광 및 기타 기술 분야.

우리의 제품은 많은 분야에서 널리 사용됩니다., 의료 테스트와 같은, 유전자 검사, 대학 연구, 유전자 육종, 등. 우리는 제품을 제공할 뿐만 아니라 OEM도 수행할 수 있습니다., ODM, 그리고 다른 필요. 관련 요구사항이 있는 경우, 언제든지 sales01@lingjunbio.com으로 문의해 주세요..