Lnjnbio magnetiske perler for DNA/RNA-ekstraksjonssett for animalsk vev 231012

(Magnetisk perlemetode)

【Produktintroduksjon】

This kit uses magnetic beads and buffer system with unique separation effect to extract DNA/RNA from animal tissues, the whole process is easy to operate especially suitable for automated nucleic acid extraction platform. The extracted nucleic acids are of high yield, høy renhet, stabil og pålitelig kvalitet, and can be applied to various downstream molecular biology experiments.

【Produktfordeler】

1, Simple and fast: direct lysis, no need to incubate for long time, high quality DNA/RNA can be obtained within 30 min

2, Safe process: no liquid nitrogen grinding of samples

3, Safe and non-toxic: no organic reagents such as phenol/chloroform required

4, Wide range of applications: applicable to a wide range of animal tissues, successfully extracted from pork, beef, fish, shrimp, porcine and other samples to obtain DNA / RNA

5, High-throughput: can be used with automated nucleic acid extraction platforms to realize high-throughput extraction

【Produktbilde】

【Eksperimentell sak】

Sak: Different tissue samples from different animals were extracted for DNA/RNA and the results were as follows:

Number	Prøve	A260/A280	A260/A230	Kons.(ng/μL)	Avkastning(μg)	CV
1	20mgFish	1.856	1.884	109.25	10.925
2		1.866	1.956	117.4	11.74
Average		1.861	1.92	113.325	11.3325	0.28%
3	20mg Chicken meat	1.886	2.046	284.55	28.455
4		1.889	2.041	249.4	24.94
Average		1.8875	2.0435	266.975	26.6975	6.58%
5	5mg Fish liver	1.806	1.587	256.45	25.645
6		1.806	1.572	253.4	25.34
Average		1.806	1.5795	254.925	25.4925	0.60%
7	5mg Chicken heart	1.856	1.966	101.95	10.195
8		1.85	1.999	107.2	10.72
Average		1.853	1.9825	104.575	10.4575	2.51%

Toppmønsterdiagram:

Fish：

Chicken meat:

Fish liver：

Chicken heart：

Total peak shape：

Elektroferogrammet er vist nedenfor:

Resultatanalyse:

Resultatene av Ultra-Micro Nucleic Acid Assay (UMNA) test viste det:

①A260/A280, A260/A230, the coefficient of variation was below 6.58%, which proved the high stability of reagent extraction.

②The values of A260/A280 were above 1.8, and the values of A260/A230 were in the range of 1.5-2.0, and the peak shapes were complete and basically overlapped proving that the extracted products were of high purity.

③Nucleic acid extraction yields ranged from 10-28 µg or more for different animal tissue samples.

Resultatene av agarosegelelektroforese viste det:

①Different tissue samples from different animals have bright bands with good integrity.

Som konklusjon: the nucleic acids of different animal samples were extracted by the fully automatic nucleic acid extraction and purification instrument, and the extracted products were of high purity, høyt utbytte, complete electrophoretic bands, and the reagents were uniform and stable in cooperation with the instrument.

【ENbruksområde】

Nucleic acids purified by this kit can be used for PCR, qPCR, digestion, library construction and Southern blot.

Leverandørintroduksjon

Shanghai Lingjun Biotechnology Co., Ltd.ble etablert i 2016 og er en profesjonell produsent av biomagnetiske materialer og nukleinsyreekstraksjonsreagenser.

Vi har rik erfaring innen utvinning og rensing av nukleinsyre, proteinrensing, celleseparasjon, kjemiluminescens og andre tekniske felt.

Våre produkter er mye brukt på mange felt, som medisinsk testing, genetisk testing, universitetsforskning, genetisk avl, og så videre. Vi leverer ikke bare produkter, men kan også ta OEM, ODM, og andre behov. Hvis du har relaterte behov, Ta gjerne kontakt med oss ​​på sales01@lingjunbio.com.