Serum/Plasma Viral Nucleic Acid Extraction Kit 221001

(마그네틱 비드 방식)

【제품소개】

This product uses guanidine salt to directly lyse cells and pathogens (바이러스, 박테리아, mycoplasma, chlamydia) in serum/plasma, 핵산 방출. Magnetic beads selectively adsorb nucleic acids in the lysis solution under the action of isopropanol. Remove a small amount of impurities adsorbed by magnetic beads through cleaning with detergent. Under the action of eluent, magnetic beads release adsorbed nucleic acids to obtain high-quality viral DNA/RNA. The virus DNA/RNA extracted from serum/plasma samples using this product does not require toxic reagents such as phenol or chloroform, and the obtained virus DNA/RNA can be directly used in downstream experiments.

【제품의 장점】

1、Broad adaptability: Suitable for a wide range of samples including plasma, 혈청, and other cell-free bodily fluids, both DNA and RNA viruses are applicable.

2、Adopting independently developed and produced magnetic beads,the extracted product can carry magnetic beads for PCR amplification.

3、High sensitivity: 10 IU/mL viral nucleic acid can be stably extracted from 200 µL serum or plasma samples containing viruses.

4、High universality: can cooperate with automated nucleic acid extraction equipment from different manufacturers for high-throughput operations.

【상품 이미지】

【실험사례】

사례 1:

Using our serum/plasma virus nucleic acid extraction reagents, HBV national standard gradient diluents were extracted on the automatic nucleic acid extraction and purification instrument LJbio32H. The extracted products of each concentration gradient diluent were detected by HBV-qPCR, with a correlation coefficient R>0.99. The specific data is as follows:

결과 분석：The accuracy of the reagent is very high, and the sensitivity is still good when extracting low concentration samples.

사례 2:

Dilute the HBV national standard to 10 IU/mL, and use our serum/plasma virus nucleic acid extraction reagent to perform 20 repeated extractions on the automatic nucleic acid extraction and purification instrument LJbio32H. The extracted products are tested for HBV-qPCR and can be stably detected.

결과 분석：The small difference between duplicate samples indicates stable reagent performance and good reproducibility of results.

사례 3：

HCV nucleic acid serum standard substance was subjected to gradient dilution, and our serum/plasma virus nucleic acid extraction reagent was used to extract on the automatic nucleic acid extraction and purification instrument LJbio32H. The extracted products of various concentration gradient dilutions were subjected to qPCR detection, and the results are as follows:

Concentration（IU/ml）	CT value		Average CT value	CV value
10^7	23.02	22.96	22.99	0.18%
10^6	25.88	25.83	25.855	0.14%
10^5	29.09	28.97	29.03	0.29%
10^4	33.16	32.42	32.79	1.60%

결과 분석: The data shows high consistency, indicating excellent sensitivity and stable reagent performance, with a correlation coefficient reaching 0.998. The extraction efficiency is remarkable, consistently detecting both low and high concentrations with stability.

Case 4：Extract reagents from a domestic manufacturer for virus detection experiments (extract positive samples of swine fever virus from serum samples)

결과 분석: Whether it is high concentration or low concentration samples, the CT value of our extraction reagent is significantly advanced, indicating that the extraction effect and sensitivity of our extraction reagent are significantly better.

【에이pplication area】

The nucleic acids purified by this extraction reagent are suitable for various precision downstream experiments such as RT-PCR detection and qRT-PCR detection.

공급업체 소개

상하이 링쥔 생명공학 유한회사, 주식회사에 설립되었습니다 2016 생체자성재료 및 핵산추출시약 전문제조업체입니다..

핵산 추출 및 정제 분야에서 풍부한 경험을 보유하고 있습니다., 단백질 정제, 세포 분리, 화학발광 및 기타 기술 분야.

우리의 제품은 많은 분야에서 널리 사용됩니다., 의료 테스트와 같은, 유전자 검사, 대학 연구, 유전자 육종, 등. 우리는 제품을 제공할 뿐만 아니라 OEM도 수행할 수 있습니다., ODM, 그리고 다른 필요. 관련 요구사항이 있는 경우, 언제든지 sales01@lingjunbio.com으로 문의해 주세요..