Kit de extracción de ácido nucleico viral en suero/plasma 221001

(Método de cuentas magnéticas)

【Introducción del producto】

This product uses guanidine salt to directly lyse cells and pathogens (viruses, bacteria, mycoplasma, chlamydia) in serum/plasma, releasing nucleic acids. Magnetic beads selectively adsorb nucleic acids in the lysis solution under the action of isopropanol. Remove a small amount of impurities adsorbed by magnetic beads through cleaning with detergent. Under the action of eluent, magnetic beads release adsorbed nucleic acids to obtain high-quality viral DNA/RNA. The virus DNA/RNA extracted from serum/plasma samples using this product does not require toxic reagents such as phenol or chloroform, and the obtained virus DNA/RNA can be directly used in downstream experiments.

【Ventajas del producto】

1、Broad adaptability: Suitable for a wide range of samples including plasma, suero, and other cell-free bodily fluids, both DNA and RNA viruses are applicable.

2、Adopting independently developed and produced magnetic beads,the extracted product can carry magnetic beads for PCR amplification.

3、High sensitivity: 10 IU/mL viral nucleic acid can be stably extracted from 200 µL serum or plasma samples containing viruses.

4、High universality: can cooperate with automated nucleic acid extraction equipment from different manufacturers for high-throughput operations.

【Imagen del producto】

【Casos experimentales】

Caso 1:

Using our serum/plasma virus nucleic acid extraction reagents, HBV national standard gradient diluents were extracted on the automatic nucleic acid extraction and purification instrument LJbio32H. The extracted products of each concentration gradient diluent were detected by HBV-qPCR, with a correlation coefficient R>0.99. The specific data is as follows:

Análisis de resultados：The accuracy of the reagent is very high, and the sensitivity is still good when extracting low concentration samples.

Caso 2:

Dilute the HBV national standard to 10 IU/mL, and use our serum/plasma virus nucleic acid extraction reagent to perform 20 repeated extractions on the automatic nucleic acid extraction and purification instrument LJbio32H. The extracted products are tested for HBV-qPCR and can be stably detected.

Análisis de resultados：The small difference between duplicate samples indicates stable reagent performance and good reproducibility of results.

Caso 3：

HCV nucleic acid serum standard substance was subjected to gradient dilution, and our serum/plasma virus nucleic acid extraction reagent was used to extract on the automatic nucleic acid extraction and purification instrument LJbio32H. The extracted products of various concentration gradient dilutions were subjected to qPCR detection, y los resultados son los siguientes:

Concentration（IU/ml）	CT value		Average CT value	CV value
10^7	23.02	22.96	22.99	0.18%
10^6	25.88	25.83	25.855	0.14%
10^5	29.09	28.97	29.03	0.29%
10^4	33.16	32.42	32.79	1.60%

Análisis de resultados: The data shows high consistency, indicating excellent sensitivity and stable reagent performance, with a correlation coefficient reaching 0.998. The extraction efficiency is remarkable, consistently detecting both low and high concentrations with stability.

Case 4：Extract reagents from a domestic manufacturer for virus detection experiments (extract positive samples of swine fever virus from serum samples)

Análisis de resultados: Whether it is high concentration or low concentration samples, the CT value of our extraction reagent is significantly advanced, indicating that the extraction effect and sensitivity of our extraction reagent are significantly better.

【Application area】

The nucleic acids purified by this extraction reagent are suitable for various precision downstream experiments such as RT-PCR detection and qRT-PCR detection.

Introducción del proveedor

Shanghai Lingjun Biotecnología Co., Limitado.se estableció en 2016 y es un fabricante profesional de materiales biomagnéticos y reactivos de extracción de ácidos nucleicos..

Tenemos una rica experiencia en extracción y purificación de ácidos nucleicos., purificación de proteínas, separación celular, quimioluminiscencia y otros campos técnicos.

Nuestros productos son ampliamente utilizados en muchos campos., como pruebas médicas, pruebas genéticas, investigación universitaria, mejoramiento genético, etcétera. No solo proporcionamos productos sino que también podemos realizar OEM, ODM, y otras necesidades. Si tienes necesidades relacionadas, no dude en contactarnos en sales01@lingjunbio.com.