Kit Pengekstrakan Asid Nukleik Virus Serum/Plasma 221001

(Kaedah Manik Magnet)

【Pengenalan Produk】

This product uses guanidine salt to directly lyse cells and pathogens (virus, bakteria, mycoplasma, chlamydia) in serum/plasma, releasing nucleic acids. Magnetic beads selectively adsorb nucleic acids in the lysis solution under the action of isopropanol. Remove a small amount of impurities adsorbed by magnetic beads through cleaning with detergent. Under the action of eluent, magnetic beads release adsorbed nucleic acids to obtain high-quality viral DNA/RNA. The virus DNA/RNA extracted from serum/plasma samples using this product does not require toxic reagents such as phenol or chloroform, and the obtained virus DNA/RNA can be directly used in downstream experiments.

【Kelebihan produk】

1、Broad adaptability: Suitable for a wide range of samples including plasma, serum, and other cell-free bodily fluids, both DNA and RNA viruses are applicable.

2、Adopting independently developed and produced magnetic beads,the extracted product can carry magnetic beads for PCR amplification.

3、Kepekaan tinggi: 10 IU/mL viral nucleic acid can be stably extracted from 200 µL serum or plasma samples containing viruses.

4、High universality: can cooperate with automated nucleic acid extraction equipment from different manufacturers for high-throughput operations.

【Imej produk】

【Kes eksperimen】

Kes 1:

Using our serum/plasma virus nucleic acid extraction reagents, HBV national standard gradient diluents were extracted on the automatic nucleic acid extraction and purification instrument LJbio32H. The extracted products of each concentration gradient diluent were detected by HBV-qPCR, with a correlation coefficient R>0.99. The specific data is as follows:

Analisis keputusan：The accuracy of the reagent is very high, and the sensitivity is still good when extracting low concentration samples.

Kes 2:

Dilute the HBV national standard to 10 IU/mL, and use our serum/plasma virus nucleic acid extraction reagent to perform 20 repeated extractions on the automatic nucleic acid extraction and purification instrument LJbio32H. The extracted products are tested for HBV-qPCR and can be stably detected.

Analisis keputusan：The small difference between duplicate samples indicates stable reagent performance and good reproducibility of results.

Kes 3：

Bahan standard serum asid nukleik HCV telah tertakluk kepada pencairan kecerunan, dan reagen pengekstrakan asid nukleik virus serum/plasma kami digunakan untuk mengekstrak pada alat pengekstrakan dan penulenan asid nukleik automatik LJbio32H. Produk yang diekstrak daripada pelbagai pencairan kecerunan kepekatan tertakluk kepada pengesanan qPCR, dan hasilnya adalah seperti berikut:

Concentration（IU/ml）	CT value		Average CT value	CV value
10^7	23.02	22.96	22.99	0.18%
10^6	25.88	25.83	25.855	0.14%
10^5	29.09	28.97	29.03	0.29%
10^4	33.16	32.42	32.79	1.60%

Analisis keputusan: Data menunjukkan konsistensi yang tinggi, menunjukkan kepekaan yang sangat baik dan prestasi reagen yang stabil, dengan pekali korelasi mencapai 0.998. Kecekapan pengekstrakan adalah luar biasa, secara konsisten mengesan kedua-dua kepekatan rendah dan tinggi dengan kestabilan.

Case 4：Extract reagents from a domestic manufacturer for virus detection experiments (extract positive samples of swine fever virus from serum samples)

Analisis keputusan: Whether it is high concentration or low concentration samples, the CT value of our extraction reagent is significantly advanced, indicating that the extraction effect and sensitivity of our extraction reagent are significantly better.

【Akawasan aplikasi】

The nucleic acids purified by this extraction reagent are suitable for various precision downstream experiments such as RT-PCR detection and qRT-PCR detection.

Pengenalan Pembekal

Shanghai Lingjun Biotechnology Co., Ltd.ditubuhkan pada 2016 dan merupakan pengilang profesional bahan biomagnet dan reagen pengekstrakan asid nukleik.

Kami mempunyai pengalaman yang kaya dalam pengekstrakan dan penulenan asid nukleik, penulenan protein, pemisahan sel, chemiluminescence dan bidang teknikal lain.

Produk kami digunakan secara meluas dalam pelbagai bidang, seperti ujian perubatan, ujian genetik, penyelidikan universiti, pembiakan genetik, dan seterusnya. Kami bukan sahaja menyediakan produk tetapi juga boleh menjalankan OEM, ODM, dan keperluan lain. Jika anda mempunyai keperluan yang berkaitan, sila hubungi kami di sales01@lingjunbio.com.