Kit Ekstraksi Asam Nukleat Virus Serum/Plasma 221001

(Metode Manik Magnetik)

【Pengenalan Produk】

This product uses guanidine salt to directly lyse cells and pathogens (virus, bakteri, mikoplasma, chlamydia) in serum/plasma, releasing nucleic acids. Magnetic beads selectively adsorb nucleic acids in the lysis solution under the action of isopropanol. Remove a small amount of impurities adsorbed by magnetic beads through cleaning with detergent. Under the action of eluent, magnetic beads release adsorbed nucleic acids to obtain high-quality viral DNA/RNA. The virus DNA/RNA extracted from serum/plasma samples using this product does not require toxic reagents such as phenol or chloroform, dan yang diperoleh virus DNA/RNA can be directly used in downstream experiments.

【Keunggulan produk】

1、Broad adaptability: Suitable for a wide range of samples including plasma, serum, and other cell-free bodily fluids, both DNA and RNA viruses are applicable.

2、Adopting independently developed and produced magnetic beads,the extracted product can carry magnetic beads for PCR amplification.

3、High sensitivity: 10 IU/mL viral nucleic acid can be stably extracted from 200 µL serum or plasma samples containing viruses.

4、High universality: can cooperate with automated nucleic acid extraction equipment from different manufacturers for high-throughput operations.

【Gambar produk】

【Kasus eksperimental】

Kasus 1:

Using our serum/plasma virus nucleic acid extraction reagents, HBV national standard gradient diluents were extracted on the automatic nucleic acid extraction and purification instrument LJbio32H. The extracted products of each concentration gradient diluent were detected by HBV-qPCR, with a correlation coefficient R>0.99. The specific data is as follows:

Analisis hasil：The accuracy of the reagent is very high, and the sensitivity is still good when extracting low concentration samples.

Kasus 2:

Dilute the HBV national standard to 10 IU/mL, and use our serum/plasma virus nucleic acid extraction reagent to perform 20 repeated extractions on the automatic nucleic acid extraction and purification instrument LJbio32H. The extracted products are tested for HBV-qPCR and can be stably detected.

Analisis hasil：The small difference between duplicate samples indicates stable reagent performance and good reproducibility of results.

Kasus 3：

Zat standar serum asam nukleat HCV dikenai pengenceran gradien, dan reagen ekstraksi asam nukleat virus serum/plasma kami digunakan untuk mengekstraksi pada instrumen ekstraksi dan pemurnian asam nukleat otomatis LJbio32H. Produk yang diekstraksi dari berbagai pengenceran gradien konsentrasi dikenai deteksi qPCR, dan hasilnya sebagai berikut:

Concentration（IU/ml）	CT value		Average CT value	CV value
10^7	23.02	22.96	22.99	0.18%
10^6	25.88	25.83	25.855	0.14%
10^5	29.09	28.97	29.03	0.29%
10^4	33.16	32.42	32.79	1.60%

Analisis hasil: Data menunjukkan konsistensi yang tinggi, menunjukkan sensitivitas yang sangat baik dan kinerja reagen yang stabil, dengan koefisien korelasi mencapai 0.998. Efisiensi ekstraksinya luar biasa, secara konsisten mendeteksi konsentrasi rendah dan tinggi dengan stabilitas.

Case 4：Extract reagents from a domestic manufacturer for virus detection experiments (extract positive samples of swine fever virus from serum samples)

Analisis hasil: Whether it is high concentration or low concentration samples, the CT value of our extraction reagent is significantly advanced, indicating that the extraction effect and sensitivity of our extraction reagent are significantly better.

【Aarea aplikasi】

The nucleic acids purified by this extraction reagent are suitable for various precision downstream experiments such as RT-PCR detection and qRT-PCR detection.

Pengenalan Pemasok

Shanghai Lingjun Bioteknologi Co., Ltd.didirikan pada 2016 dan merupakan produsen profesional bahan biomagnetik dan reagen ekstraksi asam nukleat.

Kami memiliki pengalaman yang kaya dalam ekstraksi dan pemurnian asam nukleat, pemurnian protein, pemisahan sel, chemiluminescence dan bidang teknis lainnya.

Produk kami banyak digunakan di berbagai bidang, misalnya tes kesehatan, pengujian genetik, penelitian universitas, pemuliaan genetik, dan sebagainya. Kami tidak hanya menyediakan produk tetapi juga dapat melakukan OEM, ODM, dan kebutuhan lainnya. Jika Anda memiliki kebutuhan terkait, jangan ragu untuk menghubungi kami di sales01@lingjunbio.com.