Serum/Plasma Viral Nucleic Acid Extraction Kit 221001

(磁気ビーズ法)

【Product Introduction】

This product uses guanidine salt to directly lyse cells and pathogens (ウイルス, bacteria, mycoplasma, chlamydia) in serum/plasma, releasing nucleic acids. Magnetic beads selectively adsorb nucleic acids in the lysis solution under the action of isopropanol. Remove a small amount of impurities adsorbed by magnetic beads through cleaning with detergent. Under the action of eluent, magnetic beads release adsorbed nucleic acids to obtain high-quality viral DNA/RNA. The virus DNA/RNA extracted from serum/plasma samples using this product does not require toxic reagents such as phenol or chloroform, and the obtained virus DNA/RNA can be directly used in downstream experiments.

【Product advantages】

1、Broad adaptability: Suitable for a wide range of samples including plasma, 血清, and other cell-free bodily fluids, both DNA and RNA viruses are applicable.

2、Adopting independently developed and produced magnetic beads,the extracted product can carry magnetic beads for PCR amplification.

3、High sensitivity: 10 IU/mL viral nucleic acid can be stably extracted from 200 µL serum or plasma samples containing viruses.

4、High universality: can cooperate with automated nucleic acid extraction equipment from different manufacturers for high-throughput operations.

【Product image】

【Experimental cases】

場合 1:

Using our serum/plasma virus nucleic acid extraction reagents, HBV national standard gradient diluents were extracted on the automatic nucleic acid extraction and purification instrument LJbio32H. The extracted products of each concentration gradient diluent were detected by HBV-qPCR, with a correlation coefficient R>0.99. The specific data is as follows:

Result analysis：The accuracy of the reagent is very high, and the sensitivity is still good when extracting low concentration samples.

場合 2:

Dilute the HBV national standard to 10 IU/mL, and use our serum/plasma virus nucleic acid extraction reagent to perform 20 repeated extractions on the automatic nucleic acid extraction and purification instrument LJbio32H. The extracted products are tested for HBV-qPCR and can be stably detected.

Result analysis：The small difference between duplicate samples indicates stable reagent performance and good reproducibility of results.

場合 3：

HCV nucleic acid serum standard substance was subjected to gradient dilution, and our serum/plasma virus nucleic acid extraction reagent was used to extract on the automatic nucleic acid extraction and purification instrument LJbio32H. The extracted products of various concentration gradient dilutions were subjected to qPCR detection, そして結果は次のとおりです:

Concentration（IU/ml）	CT value		Average CT value	CV value
10^7	23.02	22.96	22.99	0.18%
10^6	25.88	25.83	25.855	0.14%
10^5	29.09	28.97	29.03	0.29%
10^4	33.16	32.42	32.79	1.60%

Result analysis: The data shows high consistency, indicating excellent sensitivity and stable reagent performance, with a correlation coefficient reaching 0.998. The extraction efficiency is remarkable, consistently detecting both low and high concentrations with stability.

Case 4：Extract reagents from a domestic manufacturer for virus detection experiments (extract positive samples of swine fever virus from serum samples)

Result analysis: Whether it is high concentration or low concentration samples, the CT value of our extraction reagent is significantly advanced, indicating that the extraction effect and sensitivity of our extraction reagent are significantly better.

【あpplication area】

The nucleic acids purified by this extraction reagent are suitable for various precision downstream experiments such as RT-PCR detection and qRT-PCR detection.

サプライヤー紹介

上海霊軍生物技術有限公司, 株式会社に設立されました 2016 and is a professional manufacturer of biomagnetic materials and nucleic acid extraction reagents.

核酸抽出・精製の経験が豊富です。, タンパク質の精製, 細胞分離, 化学発光およびその他の技術分野.

当社の製品はさまざまな分野で広く使用されています, 医療検査など, 遺伝子検査, 大学の研究, 遺伝子育種, 等々. We not only provide products but can also undertake OEM, ODM, その他のニーズ. 関連するニーズがある場合, please feel free to contact us at sales01@lingjunbio.com.