血清/血漿ウイルス核酸抽出キット 221001

(磁気ビーズ法)

【商品紹介】

This product uses guanidine salt to directly lyse cells and pathogens (ウイルス, 細菌, マイコプラズマ, chlamydia) in serum/plasma, 核酸を放出する. Magnetic beads selectively adsorb nucleic acids in the lysis solution under the action of isopropanol. Remove a small amount of impurities adsorbed by magnetic beads through cleaning with detergent. Under the action of eluent, magnetic beads release adsorbed nucleic acids to obtain high-quality viral DNA/RNA. The virus DNA/RNA extracted from serum/plasma samples using this product does not require toxic reagents such as phenol or chloroform, and the obtained virus DNA/RNA can be directly used in downstream experiments.

【製品の利点】

1、Broad adaptability: Suitable for a wide range of samples including plasma, 血清, and other cell-free bodily fluids, both DNA and RNA viruses are applicable.

2、Adopting independently developed and produced magnetic beads,the extracted product can carry magnetic beads for PCR amplification.

3、高感度: 10 IU/mL viral nucleic acid can be stably extracted from 200 µL serum or plasma samples containing viruses.

4、High universality: can cooperate with automated nucleic acid extraction equipment from different manufacturers for high-throughput operations.

【商品画像】

【実験事例】

場合 1:

Using our serum/plasma virus nucleic acid extraction reagents, HBV national standard gradient diluents were extracted on the automatic nucleic acid extraction and purification instrument LJbio32H. The extracted products of each concentration gradient diluent were detected by HBV-qPCR, with a correlation coefficient R>0.99. The specific data is as follows:

結果分析：The accuracy of the reagent is very high, and the sensitivity is still good when extracting low concentration samples.

場合 2:

Dilute the HBV national standard to 10 IU/mL, and use our serum/plasma virus nucleic acid extraction reagent to perform 20 repeated extractions on the automatic nucleic acid extraction and purification instrument LJbio32H. The extracted products are tested for HBV-qPCR and can be stably detected.

結果分析：The small difference between duplicate samples indicates stable reagent performance and good reproducibility of results.

場合 3：

HCV核酸血清標準物質を勾配希釈した, 当社の血清・血漿ウイルス核酸抽出試薬を用いて自動核酸抽出精製装置LJbio32Hで抽出しました. さまざまな濃度勾配希釈の抽出産物をqPCR検出に供した, そして結果は次のとおりです:

Concentration（IU/ml）	CT value		Average CT value	CV value
10^7	23.02	22.96	22.99	0.18%
10^6	25.88	25.83	25.855	0.14%
10^5	29.09	28.97	29.03	0.29%
10^4	33.16	32.42	32.79	1.60%

結果分析: データは高い一貫性を示しています, 優れた感度と安定した試薬性能を示します, 相関係数が以下に達する 0.998. 抽出効率は抜群です, 低濃度から高濃度まで安定して検出.

Case 4：Extract reagents from a domestic manufacturer for virus detection experiments (extract positive samples of swine fever virus from serum samples)

結果分析: Whether it is high concentration or low concentration samples, the CT value of our extraction reagent is significantly advanced, indicating that the extraction effect and sensitivity of our extraction reagent are significantly better.

【あ適用範囲】

The nucleic acids purified by this extraction reagent are suitable for various precision downstream experiments such as RT-PCR detection and qRT-PCR detection.

サプライヤー紹介

上海霊軍生物技術有限公司, 株式会社に設立されました 2016 生体磁性材料と核酸抽出試薬の専門メーカーです.

核酸抽出・精製の経験が豊富です。, タンパク質の精製, 細胞分離, 化学発光およびその他の技術分野.

当社の製品はさまざまな分野で広く使用されています, 医療検査など, 遺伝子検査, 大学の研究, 遺伝子育種, 等々. 製品のご提供だけでなく、OEMも承ります。, ODM, その他のニーズ. 関連するニーズがある場合, sales01@lingjunbio.comまでお気軽にお問い合わせください。.