Lnjnbio Magnetic Beads for Ultra-trace Amount of Cells DNA/RNA Extraction Kit231013

Ultra-trace Amount of Cells DNA/RNA Extraction Kit

 (磁気ビーズ法)

 (磁気ビーズ法)
【Product Introduction】

Various ultra trace cell samples, のような: cells, tissue homogenate, cell culture fluid, exfoliated cells, 尿, cerebrospinal fluid, secretion and other samples, under the action of guanidinium salts are lysed and release nucleic acid, through the specific adsorption of the magnetic beads to separate the nucleic acid from other impurities in the samples, and then rinsed by the washing solution, to remove the proteins, residual guanidinium salts, 等, and finally, after the elution, to obtain a good purification of the nucleic acid, the obtained The nucleic acid product can be used for PCR amplification, nucleic acid detection and analysis, gene mutation detection and sequencing and other experiments.

【Product advantages】

1、It is suitable for trace samples with large volume and low nucleic acid concentration, micro tissue samples and cultured cells (small amount of cells) samples.

2、Using the specific adsorption property of magnetic beads, the nucleic acid recovery rate is high, no need for centrifugation, easy to operate, the whole extraction process takes 10-30 min.

3、Saving samples, get nucleic acid extraction product volume of 2-20 μL, to ensure the concentration of nucleic acid extracted from trace samples and rare samples, very suitable for rare and precious small amount of cell samples nucleic acid extraction.

4、The whole genome and the whole transcriptome can be extracted at the same time, which can obtain the complete nucleic acid information of a small number of cells with relatively high concentration, and can be directly docked to the third-generation sequencer.

5、Room temperature lysis combined with one-step, nucleic acid is not easy to degrade, maximize the protection of the integrity of the nucleic acid, is very suitable for the whole genome and transcriptome extraction of a small number of 10-500 cells.

【Product image】

【Experimental cases】

場合 1: Nucleic acids obtained by extracting 0.01 mg, 0.005 mg, 0.002 mg, そして 0.001 mg of trace pork cell samples were detected by 1% agarose gel electrophoresis for nucleic acid bands, and the results obtained are shown in the figure below:

1: 0.01mg of pork cell sample extraction product

2: 0.005mg of pork cell sample extraction product

3: 0.002mg of pork cell sample extraction product

4: 0.001mg of pork cell sample extraction product

5: Blank control

Result analysis： From the results of agarose electrophoresis of the extracted products, it can be seen that 1-4 were able to run DNA electrophoretic bands, indicating that nucleic acids could be extracted from 0.01 mg, 0.005 mg, 0.002 mg, そして 0.001 mg of the trace pork cell samples.

場合 2：Nucleic acids were extracted from trace amounts of human Hela cells as samples, 5 cells, 10 cells, 20 cells, 500 cells were extracted, and the nucleic acids obtained were analyzed using Agilent 4200 Nucleic acid fragment distribution, and the results obtained are shown below: 

1. The Electropherogram of Agilent 4200 High Sensitivity RNA ScreenTape Assay

D1: 50 Hela cellsG1: 20 Hela cellsB2: 10 Hela cellsE2: 5 Hela cells

2. Peak diagram of the 50/20/10/5 Hela cells extracted

• Peak diagram of the 5 Hela cells extracted

• . Qubit’s test results

No.	Sample intake	収率（ng/μL）
1	50 Hela cells	0.242
2	20 Hela cells	0.136
3	10 Hela cells	0.112
4	5 Hela cells	0.096

Result analysis： By Agilent 4200 electrophoresis results can be seen: 5– 50 traces of human Hela cell samples, can be obtained by the nucleic acid fragments, The extraction products were 18s RNA, 28sRNA, and DNA over 800bp.

【あpplication area】

1、It can be used for early tumor screening, prognosis,s and late recurrence prediction, pregnancy/newborn genetic disease screening, and various types of disease early warning and other precision medicine applications, tracing the pathogenesis of disease, disease prevention, and diagnosis and treatment.

2、It is suitable for precision medicine to formulate treatment plans for tumor cell heterogeneity, which is suitable for the clinical needs of difficult-to-take samples during pregnancy and maternity tests, precious cells,and high accuracy of the results.

サプライヤー紹介

上海霊軍生物技術有限公司, 株式会社に設立されました 2016 生体磁性材料と核酸抽出キットの専門メーカーです.

核酸抽出・精製の経験が豊富です。, タンパク質の精製, 細胞分離, 化学発光およびその他の技術分野.

当社の製品はさまざまな分野で広く使用されています, 医療検査など, 遺伝子検査, 大学の研究, 遺伝子育種, 等々. 製品のご提供だけでなく、OEMも承ります。, ODM, その他のニーズ. 関連するニーズがある場合, sales01@lingjunbio.comまでお気軽にお問い合わせください。.