Lnjnbio Perline magnetiche per quantità ultra-tracce di cellule Kit di estrazione DNA/RNA231013

Ultra-trace Amount of Cells DNA/RNA Extraction Kit

 (Metodo della perla magnetica)

 (Metodo della perla magnetica)
【Introduzione al prodotto】

Various ultra trace cell samples, such as: cellule, tissue homogenate, cell culture fluid, exfoliated cells, urina, liquido cerebrospinale, secretion and other samples, under the action of guanidinium salts are lysed and release nucleic acid, through the specific adsorption of the magnetic beads to separate the nucleic acid from other impurities in the samples, and then rinsed by the washing solution, to remove the proteins, residual guanidinium salts, ecc., and finally, after the elution, to obtain a good purification of the nucleic acid, the obtained The nucleic acid product can be used for PCR amplification, rilevamento e analisi degli acidi nucleici, gene mutation detection and sequencing and other experiments.

【Vantaggi del prodotto】

1、It is suitable for trace samples with large volume and low nucleic acid concentration, micro tissue samples and cultured cells (small amount of cells) samples.

2、Using the specific adsorption property of magnetic beads, the nucleic acid recovery rate is high, no need for centrifugation, easy to operate, the whole extraction process takes 10-30 min.

3、Saving samples, get nucleic acid extraction product volume of 2-20 μL, to ensure the concentration of nucleic acid extracted from trace samples and rare samples, very suitable for rare and precious small amount of cell samples nucleic acid extraction.

4、The whole genome and the whole transcriptome can be extracted at the same time, which can obtain the complete nucleic acid information of a small number of cells with relatively high concentration, and can be directly docked to the third-generation sequencer.

5、Room temperature lysis combined with one-step, nucleic acid is not easy to degrade, maximize the protection of the integrity of the nucleic acid, is very suitable for the whole genome and transcriptome extraction of a small number of 10-500 cellule.

【Immagine del prodotto】

【Casi sperimentali】

Caso 1: Nucleic acids obtained by extracting 0.01 mg, 0.005 mg, 0.002 mg, E 0.001 mg of trace pork cell samples were detected by 1% agarose gel electrophoresis for nucleic acid bands, and the results obtained are shown in the figure below:

1: 0.01mg of pork cell sample extraction product

2: 0.005mg of pork cell sample extraction product

3: 0.002mg of pork cell sample extraction product

4: 0.001mg of pork cell sample extraction product

5: Blank control

Analisi dei risultati： From the results of agarose electrophoresis of the extracted products, lo si può vedere 1-4 were able to run DNA electrophoretic bands, indicating that nucleic acids could be extracted from 0.01 mg, 0.005 mg, 0.002 mg, E 0.001 mg of the trace pork cell samples.

Caso 2：Nucleic acids were extracted from trace amounts of human Hela cells as samples, 5 cellule, 10 cellule, 20 cellule, 500 cells were extracted, and the nucleic acids obtained were analyzed using Agilent 4200 Nucleic acid fragment distribution, and the results obtained are shown below: 

1. The Electropherogram of Agilent 4200 High Sensitivity RNA ScreenTape Assay

D1: 50 Hela cellsG1: 20 Hela cellsB2: 10 Hela cellsE2: 5 Hela cells

2. Peak diagram of the 50/20/10/5 Hela cells extracted

• Peak diagram of the 5 Hela cells extracted

• . Qubit’s test results

NO.	Sample intake	Yield（ng/μL）
1	50 Hela cells	0.242
2	20 Hela cells	0.136
3	10 Hela cells	0.112
4	5 Hela cells	0.096

Analisi dei risultati： By Agilent 4200 electrophoresis results can be seen: 5– 50 traces of human Hela cell samples, can be obtained by the nucleic acid fragments, The extraction products were 18s RNA, 28sRNA, and DNA over 800bp.

【UNarea di applicazione】

1、It can be used for early tumor screening, prognosis,s and late recurrence prediction, pregnancy/newborn genetic disease screening, and various types of disease early warning and other precision medicine applications, tracing the pathogenesis of disease, disease prevention, and diagnosis and treatment.

2、It is suitable for precision medicine to formulate treatment plans for tumor cell heterogeneity, which is suitable for the clinical needs of difficult-to-take samples during pregnancy and maternity tests, precious cells,and high accuracy of the results.

Introduzione del fornitore

Shanghai Lingjun Biotecnologia Co., Ltd.è stato fondato nel 2016 ed è un produttore professionale di materiali biomagnetici e kit di estrazione di acidi nucleici.

Abbiamo una ricca esperienza nell'estrazione e purificazione degli acidi nucleici, purificazione delle proteine, separazione cellulare, chemiluminescenza e altri campi tecnici.

I nostri prodotti sono ampiamente utilizzati in molti campi, come i test medici, test genetici, ricerca universitaria, allevamento genetico, e così via. Non solo forniamo prodotti, ma possiamo anche intraprendere OEM, ODM, e altre esigenze. Se hai esigenze correlate, non esitate a contattarci all'indirizzo sales01@lingjunbio.com.