Lnjnbio Perline magnetiche per tamponi Kit di estrazione dell'acido nucleico 221004(Metodo della perla magnetica)

【Introduzione al prodotto】

This product is mainly used for the extraction, enrichment and purification steps of nucleic acid in swab wash, soluzione di conservazione dei virus, saliva, sperma, urina, lymph fluid and many other body fluid samples and animal tissue cell homogenate. The sample is lysed and released nucleic acid under the action of guanidine salt, the nucleic acid is separated from other impurities in the sample by the specific adsorption of magnetic beads, then rinsed by washing solution to remove protein and residual guanidinium salt, ecc., and finally eluted to get purified nucleic acid, e quello ottenuto prodotto dell'acido nucleico can be used in experiments such as PCR amplification, rilevamento e analisi degli acidi nucleici, rilevamento di mutazioni genetiche, e sequenziamento.

【Vantaggi del prodotto】

1、 Lysis and binding with one step, after adding the sample can be on the machine for extraction, the extraction process does not need to pause to add other reagents.

2、The extracted nucleic acid has high purity and good integrity.

3、Efficient and quick, with automatic nucleic acid extractor, soltanto 9 minutes to complete the extraction process.

4、Non è necessario aggiungere la proteinasi K nel processo di estrazione, and it can be transported and stored at room temperature.

5、The reagent system has good accuracy, good reproducibility and high sensitivity.

6、Forte versatilità, può essere utilizzato con diversi produttori di apparecchiature automatizzate per l'estrazione degli acidi nucleici.

【Immagine del prodotto】

【Caso sperimentale】

A coronavirus pseudovirus was extracted on a fully automated nucleic acid extractor LJbio32H using swab-based nucleic acid extraction reagents from Shanghai Leader Biotechnology Co. The extracted products were subjected to qPCR, and the data related to the precision and sensitivity were as follows:

The results showed that the amplification curves of both high and low concentration samples showed good reproducibility. 20 samples with the lowest detection limits could be detected stably.

【Area di applicazione】

1、Detection of pathogens of respiratory tract infections

Virus screening: rapid extraction of SARS-CoV-2, influenza virus (UN / B), Virus respiratorio sinciziale (RSV), and adenovirus from nasopharyngeal swabs and oropharyngeal swabs to support PCR, thermostatic amplification and other molecular tests.

Bacterial identification: for B. pertussis, Streptococcus pneumoniae, Mycobacterium tuberculosis and other pathogens, efficiently enrich trace DNA, and improve the detection rate of culture-negative samples.

Mixed infection analysis: compatible with multiplex PCR or metagenomic sequencing (mNGS), synchronously extracting nucleic acids of a variety of pathogens from a single swab, to solve the pathogen diagnosis problem of difficult pneumonia and severe infection.

2. Neonatal and maternal and infant health monitoring

Group B Streptococcus (GBS) screening: GBS DNA from late perianal swabs to prevent neonatal sepsis and meningitis.

Congenital infection screening: for cytomegalovirus (CMV), herpes simplex virus (HSV), neonatal samples can be non-invasively obtained through oral swabs for early diagnosis of congenital infection.

3. Prevention and control of zoonoses and animal diseases

Avian influenza surveillance: viral RNA was extracted from avian throat / cloaca swabs to monitor H5N1 and H7N9 subtypes in real time.

Pet pathogen detection: used for the diagnosis of pet respiratory / digestive tract infections, such as canine distemper and cat calicivirus, to ensure the prevention and control of zoonosis.

Wildlife traceability: Virus genome data was obtained from wildlife oral swabs to support the source tracing study of emerging infectious diseases (e. G., Ebola, MERS-CoV).

4. Environmental and food hygiene monitoring

Cold chain detection: Novel Coronavirus RNA is extracted from object surface swabs to monitor the pollution risk of imported cold chain food.

Hospital infection control: extract the resistant bacterial DNA (e. G. MRSA, CRE) from the surface swab of ICU equipment to track the chain of nosocomial transmission.

Sicurezza alimentare: detect food-borne pathogenic bacteria such as Salmonella and Listeria in food processing swabs to ensure the safety of the whole industry chain.

Introduzione del fornitore

Shanghai Lingjun Biotecnologia Co., Ltd.è stato fondato nel 2016 ed è un produttore professionale di materiali biomagnetici e reagenti per l'estrazione degli acidi nucleici.

Abbiamo una ricca esperienza nell'estrazione e purificazione degli acidi nucleici, purificazione delle proteine, separazione cellulare, chemiluminescenza e altri campi tecnici.

I nostri prodotti sono ampiamente utilizzati in molti campi, come i test medici, test genetici, ricerca universitaria, allevamento genetico, e così via. Non solo forniamo prodotti, ma possiamo anche intraprendere OEM, ODM, e altre esigenze. Se hai esigenze correlate, non esitate a contattarci all'indirizzo sales01@lingjunbio.com.