Kit de extração de ácido nucleico de esferas magnéticas Lnjnbio para cotonetes 221004(Método de Conta Magnética)

【Introdução do produto】

This product is mainly used for the extraction, enrichment and purification steps of nucleic acid in swab wash, solução de preservação de vírus, saliva, sêmen, urina, lymph fluid and many other body fluid samples and animal tissue cell homogenate. The sample is lysed and released nucleic acid under the action of guanidine salt, the nucleic acid is separated from other impurities in the sample by the specific adsorption of magnetic beads, then rinsed by washing solution to remove protein and residual guanidinium salt, etc., and finally eluted to get purified nucleic acid, e o obtido produto de ácido nucleico can be used in experiments such as PCR amplification, detecção e análise de ácido nucleico, detecção de mutação genética, e sequenciamento.

【Vantagens do produto】

1、 Lise e ligação em uma única etapa, after adding the sample can be on the machine for extraction, the extraction process does not need to pause to add other reagents.

2、The extracted nucleic acid has high purity and good integrity.

3、Eficiente e rápido, with automatic nucleic acid extractor, apenas 9 minutes to complete the extraction process.

4、Não há necessidade de adicionar proteinase K no processo de extração, and it can be transported and stored at room temperature.

5、The reagent system has good accuracy, good reproducibility and high sensitivity.

6、Forte versatilidade, pode ser usado com diferentes fabricantes de equipamentos automatizados de extração de ácido nucleico.

【Imagem do produto】

【Caso experimental】

A coronavirus pseudovirus was extracted on a fully automated nucleic acid extractor LJbio32H using swab-based nucleic acid extraction reagents from Shanghai Leader Biotechnology Co. The extracted products were subjected to qPCR, and the data related to the precision and sensitivity were as follows:

The results showed that the amplification curves of both high and low concentration samples showed good reproducibility. 20 samples with the lowest detection limits could be detected stably.

【Área de aplicação】

1、Detection of pathogens of respiratory tract infections

Virus screening: rapid extraction of SARS-CoV-2, vírus da gripe (UM / B), vírus sincicial respiratório (RSV), and adenovirus from nasopharyngeal swabs and oropharyngeal swabs to support PCR, thermostatic amplification and other molecular tests.

Bacterial identification: for B. pertussis, Streptococcus pneumoniae, Mycobacterium tuberculosis and other pathogens, efficiently enrich trace DNA, and improve the detection rate of culture-negative samples.

Mixed infection analysis: compatible with multiplex PCR or metagenomic sequencing (mNGS), synchronously extracting nucleic acids of a variety of pathogens from a single swab, to solve the pathogen diagnosis problem of difficult pneumonia and severe infection.

2. Neonatal and maternal and infant health monitoring

Group B Streptococcus (GBS) screening: GBS DNA from late perianal swabs to prevent neonatal sepsis and meningitis.

Congenital infection screening: for cytomegalovirus (CMV), herpes simplex virus (HSV), neonatal samples can be non-invasively obtained through oral swabs for early diagnosis of congenital infection.

3. Prevention and control of zoonoses and animal diseases

Avian influenza surveillance: viral RNA was extracted from avian throat / cloaca swabs to monitor H5N1 and H7N9 subtypes in real time.

Pet pathogen detection: used for the diagnosis of pet respiratory / digestive tract infections, such as canine distemper and cat calicivirus, to ensure the prevention and control of zoonosis.

Wildlife traceability: Virus genome data was obtained from wildlife oral swabs to support the source tracing study of emerging infectious diseases (e. g., Ebola, MERS-CoV).

4. Environmental and food hygiene monitoring

Cold chain detection: Novel Coronavirus RNA is extracted from object surface swabs to monitor the pollution risk of imported cold chain food.

Hospital infection control: extract the resistant bacterial DNA (e. g. MRSA, CRE) from the surface swab of ICU equipment to track the chain of nosocomial transmission.

Segurança alimentar: detect food-borne pathogenic bacteria such as Salmonella and Listeria in food processing swabs to ensure the safety of the whole industry chain.

Introdução ao fornecedor

Xangai Lingjun Biotecnologia Co., Ltda.foi estabelecido em 2016 e é um fabricante profissional de materiais biomagnéticos e reagentes de extração de ácido nucleico.

Temos vasta experiência em extração e purificação de ácidos nucleicos, purificação de proteínas, separação celular, quimioluminescência e outros campos técnicos.

Nossos produtos são amplamente utilizados em muitos campos, como exames médicos, testes genéticos, pesquisa universitária, melhoramento genético, e assim por diante. Nós não apenas fornecemos produtos, mas também podemos realizar OEM, ODM, e outras necessidades. Se você tiver necessidades relacionadas, não hesite em contactar-nos em sales01@lingjunbio.com.