Kit universal de extração de RNA total (Pequeno volume) (Método de Conta Magnética)

【Introdução do produto】

This universal total RNA extraction kit for small volumes is designed for the rapid extraction of total RNA from a wide range of tissue samples, including fresh plant, animal, fungal and cellular materials. The entire process is straightforward to perform and is particularly well-suited for automated nucleic acid extraction platforms. During extraction, nucleic acids are released from the sample under the action of the lysis buffer; nano-magnetic beads then selectively adsorb the nucleic acids in the binding buffer, separating them from other impurities in the sample; The samples are then washed with a washing buffer to remove proteins, polysaccharides, inorganic salts and other contaminants; high-quality RNA is subsequently eluted. This kit does not require the use of toxic reagents such as phenol or chloroform. The extracted RNA yields high yields, high purity and consistent, reliable quality, making it suitable for a wide range of downstream molecular biology experiments.

【Vantagens do produto】

• Highly versatile, suitable for extracting total RNA from a wide range of tissue samples, including animal, plant, fungal and cellular sources.
• Simple to use; the magnetic bead method can be combined with fully automated nucleic acid extraction systems to enable automated extraction, with lysis and binding carried out in a single step for highly efficient extraction.
• No repeated centrifugation is required, ensuring the integrity of the extracted RNA.
• The extraction product is of high purity and yield, with intact electrophoresis bands.
• Safe to use, with no need for liquid nitrogen grinding; the product contains no harmful reagents such as phenol or chloroform.

【Experimental case】

Caso 1: RNA was extracted from various fresh tissue samples, with the following results:

No.	Sample	Sample intake	A260/A280	A260/A230	Conc.(ng/μL)	Yield（μg）
1	baby bok choy leaves	10mg	2.073	2.112	109.35	10.9
2	lettuce leaves		2.01	1.974	114.8	11.5
3	Bok Choy leaves		2.109	2.045	79.45	8
4	Pea Sprout		2.053	1.972	108.75	10.9

The electrophoresis gel is shown below：

Analysis of Results:

Test results from the ultra-micro nucleic acid analyser indicate that:

① The A260/A280 ratio ranges from 1.8 to 2.0, and the A260/A230 ratio is above 1.6, demonstrating the high purity of the extracted product.

② The yield of nucleic acid extracted from different samples is 6 µg or more.

Agarose gel electrophoresis results indicate:

① All samples exhibited bright, well-defined RNA bands with good integrity.

Resumindo: RNA extracted from various samples using this kit demonstrated high purity, high yield and intact electrophoresis bands.

Caso 2: Total RNA was extracted from various fresh leaves and tissue samples, with the following results:

No	Sample	Intake	A260/A280	A260/A230	Conc.(ng/μL)	Yield（μg）
1	Spinach leaves	10mg	1.996	2.112	113.35	11.3
2	Coriander leaves		1.955	1.936	58.95	6
3	Cabbage leaves		2.001	2.102	130.35	13
4	Celery leaves		1.999	2.142	77.55	7.8
5	Pork liver	5mg	1.884	1.713	88.75	8.9
6	Pork liver	10mg	1.872	1.688	112.05	11.2

The electrophoresis gel is shown below：

Analysis of Results:

Test results from the ultra-micro nucleic acid analyser indicate that:

① The A260/A280 ratio ranges from 1.8 to 2.0, and the A260/A230 ratio is above 1.6, demonstrating the high purity of the extracted product.

② The yield of nucleic acid extracted from different samples is 6 µg or more.

Agarose gel electrophoresis results indicate:

① All samples exhibited bright, well-defined RNA bands with good integrity.

Resumindo: RNA extracted from various samples using this kit demonstrated high purity, high yield and intact electrophoresis bands.

Caso 3: Total RNA was extracted from various fresh tissue samples, with the following results:

No.	Sample	Intake	A260/A280	A260/A230	Conc.(ng/μL)	Yield（μg）
1	Spinach leaves	10mg	1.996	2.112	113.35	11.3
2	Cabbage leaves	10mg	2.001	2.102	130.35	13
3	Celery leaves	10mg	1.999	2.142	77.55	7.8
4	Scallion leaves	20mg	2.113	2.423	77.8	7.8
5	Coriander leaves	20mg	2.03	2.116	203.75	20.4
6	Mushroom	10mg	2.171	2.68	65.8	6.6
7	Enokitake	10mg	2.073	1.969	44.45	4.5
8	White beech mushroom	20mg	2.157	2.517	56.8	5.7
9	White mushroom	20mg	2.111	2.42	118.7	11.9
10	Pork	10mg	2.041	2.011	32.2	3.2
11	Pork liver	5mg	1.884	1.713	88.75	8.9
12	Pork liver	10mg	1.872	1.708	112.05	11.2

The electrophoresis gel is shown below：

Analysis of Results:

Test results from the ultra-micro nucleic acid analyser indicate that:

① The A260/A280 ratio ranges from 1.8 to 2.0, and the A260/A230 ratio is above 1.7, demonstrating the high purity of the extracted product.

② The yield of nucleic acid extracted from different samples is 3 µg or more.

Agarose gel electrophoresis results indicate:

① All samples exhibited bright, well-defined RNA bands with good integrity.

Resumindo: RNA extracted from various samples using this kit demonstrated high purity, high yield and intact electrophoresis bands.

【Areas of application】

1.Gene Expression Analysis

qRT-PCR: Quantitative detection of the expression levels of specific genes.

RNA-Seq: High-throughput sequencing for comprehensive analysis of the transcriptome.

2.Functional Genomics Research

Gene Function Studies: Investigation of gene function using small interfering RNA (e.g. siRNA, shRNA).

Gene editing: Utilising CRISPR technology to investigate gene regulatory mechanisms.

3.Disease Diagnosis and Biomarkers

Diagnóstico de doenças: Detection of specific RNA molecules for disease diagnosis.

Biomarkers: Identification of RNA markers associated with diseases.

4.Drug Development

Target screening: Identification of drug targets.

Drug Response: Assessing the impact of drugs on gene expression.

5.Evolutionary and Comparative Genomics

Evolutionary Studies: Comparing RNA sequences across different species to investigate evolutionary relationships.

Comparative Genomics: Analysing differences in gene expression under varying conditions.

6.Epigenetic Studies

RNA Modifications: Investigating RNA modifications and their functions.

Non-coding RNA: Analysing the role of non-coding RNA in gene regulation.