Grânulos magnéticos Lnjnbio para kit de extração total de RNA de tecido animal 231002

(Método de Conta Magnética)

【Introdução do produto】

This kit uses a unique separation magnetic bead and buffer system to extract RNA from animal tissues, and the whole process is easy to operate, which is especially suitable for automated nucleic acid extraction platforms. The extracted nucleic acids have high yield, alta pureza, qualidade estável e confiável, e pode ser aplicado a vários experimentos de biologia molecular a jusante.

【Vantagens do produto】

1. Easy and fast: Samples are directly lysed without liquid nitrogen grinding, and high-quality RNA can be obtained in less than 1 h without long incubation.
2. Seguro e não tóxico: No organic reagents such as phenol/chloroform are required.
3. Ampla gama de aplicações: Suitable for a wide range of animal tissues, soft tissues (por exemplo, fígado, kidney), adipose tissue (por exemplo, brain), and fibrous tissues (por exemplo, muscle), as well as more difficult samples such as mouse tails, shell tissues.
4. High-throughput: Magnetic stand manual extraction can be selected or nucleic acid extractor can be used to achieve automated, operação de alto rendimento.
5. Efficient extraction: Biomagnetic beads have a small size effect and surface effect, which can efficiently extract nucleic acids and meet the requirements of nucleic acid extraction from rare samples and trace biological samples.

【Imagem do produto】

 

【Caso experimental】

RNA was extracted from different tissue samples from different animals, e os resultados foram os seguintes:

Caso 1:

Não.	Amostra	A260/A280	A260/A230	Conc.(de/ul)
1	20mg Camarão	1.863	1.725	65.48
2		1.865	1.782	68.96
Média		1.864	1.7535	67.22
3	5mg Coração de frango	1.958	1.721	55.72
4		1.966	1.736	56.68
Média		1.962	1.7285	56.2
5	5mg Coração de peixe	1.944	1.761	28.56
6		1.946	1.742	27.84
Média		1.945	1.7515	28.2
7	5mg Fígado de peixe	1.911	2.07	91.84
8		1.912	2.061	96.44
Média		1.9115	2.0655	94.14
9	10mg Fish intestines	1.925	2.196	390.72
10		1.928	2.187	382.04
Média		1.9265	2.1915	386.38

O eletroferograma é mostrado abaixo:

M:Marcador（2K，1K，750bp，500bp，250bp，100bp）

1-2：20 mg Shrimp 3-4：5 mg Chicken heart 5-6: 5mg Coração de peixe

7-8: 5mg Fish liver 9-10：10 mg Fish intestines

Análise de resultados:

Os resultados do ensaio de ácido ultra-micronucleico (UMNA) teste mostrou que:

(1) The value of A260/A280 is 1.86-1.96, and the value of A260/A230 is above 1.75, which proves that the purity of the extracted product is high.   

(2) The yield of nucleic acid extraction from different animal tissue samples was more than 0.2 μg.

The results of electropherogram showed that:

(1) Different animal tissue samples had bright bands and good integrity.

Resumindo, the automatic nucleic acid extraction and purification instrument was used to extract different animal tissue samples, e os produtos extraídos tinham alta pureza, alto rendimento, bandas completas de eletroforese.

Case2：

Não.	Amostra	A260/A280	A260/A230	Conc.(de/ul)
1	20mg Carne de porco	1.937	1.478	32.64
2		1.946	1.473	32.76
Média		1.9415	1.4755	32.7
3	20mg de carne bovina	1.847	1.517	98.32
4		1.85	1.515	97.64
Média		1.8485	1.516	97.98
5	20mgMutton	1.884	1.453	87.04
6		1.883	1.457	87.04
Média		1.8835	1.455	87.04
7	20mgChicken	1.83	1.419	79.32
8		1.826	1.415	79.8
Média		1.828	1.417	79.56

O eletroferograma é mostrado abaixo:

M:Marcador（2K，1K，750bp，500bp，250bp，100bp）;  

1-2： 20mg Pork 3-4：20mg Beef 5-6：20mg Mutton 7-8：20mgChicken

Análise de resultados:

Os resultados do ensaio de ácido ultra-micronucleico (UMNA) teste mostrou que:

(1) The value of A260/A280 is 1.82-1.94, e o valor de A260/A230 está acima 1.4, o que comprova que a pureza do produto extraído é alta.

(2) The yield of nucleic acid extraction from different animal tissue samples was more than 2.6 μg.

Os resultados da eletroforese em gel de agarose mostraram que:

(1) Different animal tissue samples had bright bands and good integrity.

Resumindo, the automatic nucleic acid extraction and purification instrument was used to extract different animal tissue samples, e os produtos extraídos tinham alta pureza, alto rendimento, bandas completas de eletroforese.

【Área de aplicação】

Basic molecular biology studies

It is suitable for extracting high-quality total RNA from complex animal tissues such as liver, brain, muscle and muscle for gene expression analysis (e. g., qRT-PCR, RNA-seq), transcriptomic studies and exploration of non-coding RNA function. It is especially suitable for processing samples rich in lipids or collagen, and magnetic bead-specific binding to RNA can effectively remove polysaccharides, proteins and other disruptors.

Disease mechanism and diagnostic studies

In oncology, neurodegenerative diseases, etc., they were used to extract RNA from pathological tissue samples to analyze disease-related gene expression profiles. Such as cancer studies detecting mRNA variants in cancerous tissues, or dysregulation of miRNA in neural tissues.

Drug development and safety evaluation

Supporting the assessment of the effect of drugs on gene expression in target organs in pharmacodynamic studies and the expression detection of genes related to drug metabolism (e. g., CYP450 family) in toxicology experiments.

Veterinary medicine and agricultural biotechnology

It is used for the diagnosis of livestock and poultry diseases (such as African swine fever virus detection), the verification of gene editing effect in the construction of transgenic animal models, and the functional analysis of genes related to the growth and development of economic animals.

Species identification

Combined with species-specific RNA markers, which can be used for source identification of biological samples. With easy operation (automação), alta pureza (A260 / A280> 1.9) and good integrity (RIN 8), this technology has become an important supporting platform for modern molecular experiments.

Introdução ao fornecedor

Xangai Lingjun Biotecnologia Co., Ltda.foi estabelecido em 2016 e é um fabricante profissional de materiais biomagnéticos e reagentes de extração de ácido nucleico.

Temos vasta experiência em extração e purificação de ácidos nucleicos, purificação de proteínas, separação celular, quimioluminescência e outros campos técnicos.

Nossos produtos são amplamente utilizados em muitos campos, como exames médicos, testes genéticos, pesquisa universitária, melhoramento genético, e assim por diante. Nós não apenas fornecemos produtos, mas também podemos realizar OEM, ODM, e outras necessidades. Se você tiver necessidades relacionadas, não hesite em contactar-nos em sales01@lingjunbio.com.