Lnjnbio magnetische kralen voor totaal RNA-extractiekit van dierlijk weefsel 231002

(Magnetische kralenmethode)

【Productintroductie】

This kit uses a unique separation magnetic bead and buffer system to extract RNA from animal tissues, and the whole process is easy to operate, which is especially suitable for automated nucleic acid extraction platforms. The extracted nucleic acids have high yield, high purity, stable and reliable quality, and can be applied to various downstream molecular biology experiments.

【Productvoordelen】

1. Easy and fast: Samples are directly lysed without liquid nitrogen grinding, and high-quality RNA can be obtained in less than 1 h without long incubation.
2. Veilig en niet-giftig: No organic reagents such as phenol/chloroform are required.
3. Wide range of applications: Suitable for a wide range of animal tissues, soft tissues (e.g., liver, kidney), adipose tissue (e.g., brain), and fibrous tissues (e.g., muscle), as well as more difficult samples such as mouse tails, shell tissues.
4. High-throughput: Magnetic stand manual extraction can be selected or nucleic acid extractor can be used to achieve automated, high-throughput operation.
5. Efficient extraction: Biomagnetic beads have a small size effect and surface effect, which can efficiently extract nucleic acids and meet the requirements of nucleic acid extraction from rare samples and trace biological samples.

【Productafbeelding】

 

【Experimental case】

RNA was extracted from different tissue samples from different animals, and the results were as follows:

Geval 1:

No.	Steekproef	A260/A280	A260/A230	Conc.(van/ul)
1	20mg Shrimp	1.863	1.725	65.48
2		1.865	1.782	68.96
Gem		1.864	1.7535	67.22
3	5mg Chicken heart	1.958	1.721	55.72
4		1.966	1.736	56.68
Gem		1.962	1.7285	56.2
5	5mg Fish heart	1.944	1.761	28.56
6		1.946	1.742	27.84
Gem		1.945	1.7515	28.2
7	5mg Fish liver	1.911	2.07	91.84
8		1.912	2.061	96.44
Gem		1.9115	2.0655	94.14
9	10mg Fish intestines	1.925	2.196	390.72
10		1.928	2.187	382.04
Gem		1.9265	2.1915	386.38

Het elektroferogram wordt hieronder weergegeven:

M:Marker（2K，1K，750bp，500bp，250bp，100bp）

1-2：20 mg Shrimp 3-4：5 mg Chicken heart 5-6: 5mg Fish heart

7-8: 5mg Fish liver 9-10：10 mg Fish intestines

Resultaatanalyse:

The results of the Ultra-Micro Nucleic Acid Assay (UMNA) test showed that:

(1) The value of A260/A280 is 1.86-1.96, and the value of A260/A230 is above 1.75, which proves that the purity of the extracted product is high.   

(2) The yield of nucleic acid extraction from different animal tissue samples was more than 0.2 µg.

The results of electropherogram showed that:

(1) Different animal tissue samples had bright bands and good integrity.

Samengevat, the automatic nucleic acid extraction and purification instrument was used to extract different animal tissue samples, and the extracted products had high purity, hoge opbrengst, complete electrophoresis bands.

Case2：

No.	Steekproef	A260/A280	A260/A230	Conc.(van/ul)
1	20mg Pork	1.937	1.478	32.64
2		1.946	1.473	32.76
Gem		1.9415	1.4755	32.7
3	20mg Beef	1.847	1.517	98.32
4		1.85	1.515	97.64
Gem		1.8485	1.516	97.98
5	20mgMutton	1.884	1.453	87.04
6		1.883	1.457	87.04
Gem		1.8835	1.455	87.04
7	20mgChicken	1.83	1.419	79.32
8		1.826	1.415	79.8
Gem		1.828	1.417	79.56

Het elektroferogram wordt hieronder weergegeven:

M:Marker（2K，1K，750bp，500bp，250bp，100bp）;  

1-2： 20mg Pork 3-4：20mg Beef 5-6：20mg Mutton 7-8：20mgChicken

Resultaatanalyse:

The results of the Ultra-Micro Nucleic Acid Assay (UMNA) test showed that:

(1) The value of A260/A280 is 1.82-1.94, and the value of A260/A230 is above 1.4, which proves that the purity of the extracted product is high.

(2) The yield of nucleic acid extraction from different animal tissue samples was more than 2.6 µg.

The results of agarose gel electrophoresis showed that:

(1) Different animal tissue samples had bright bands and good integrity.

Samengevat, the automatic nucleic acid extraction and purification instrument was used to extract different animal tissue samples, and the extracted products had high purity, hoge opbrengst, complete electrophoresis bands.

【Toepassingsgebied】

Basic molecular biology studies

It is suitable for extracting high-quality total RNA from complex animal tissues such as liver, brain, muscle and muscle for gene expression analysis (e. g., qRT-PCR, RNA-seq), transcriptomic studies and exploration of non-coding RNA function. It is especially suitable for processing samples rich in lipids or collagen, and magnetic bead-specific binding to RNA can effectively remove polysaccharides, proteins and other disruptors.

Disease mechanism and diagnostic studies

In oncology, neurodegenerative diseases, enz., they were used to extract RNA from pathological tissue samples to analyze disease-related gene expression profiles. Such as cancer studies detecting mRNA variants in cancerous tissues, or dysregulation of miRNA in neural tissues.

Drug development and safety evaluation

Supporting the assessment of the effect of drugs on gene expression in target organs in pharmacodynamic studies and the expression detection of genes related to drug metabolism (e. g., CYP450 family) in toxicology experiments.

Veterinary medicine and agricultural biotechnology

It is used for the diagnosis of livestock and poultry diseases (such as African swine fever virus detection), the verification of gene editing effect in the construction of transgenic animal models, and the functional analysis of genes related to the growth and development of economic animals.

Species identification

Combined with species-specific RNA markers, which can be used for source identification of biological samples. With easy operation (automation), high purity (A260 / A280> 1.9) and good integrity (RIN 8), this technology has become an important supporting platform for modern molecular experiments.

Introductie van leveranciers

Shanghai Lingjun Biotechnologie Co., Ltd.werd opgericht in 2016 en is een professionele fabrikant van biomagnetische materialen en reagentia voor nucleïnezuurextractie.

We hebben een rijke ervaring in de extractie en zuivering van nucleïnezuren, eiwit zuivering, cel scheiding, chemiluminescentie en andere technische gebieden.

Onze producten worden op veel gebieden veel gebruikt, zoals medische testen, genetische testen, universitair onderzoek, genetische veredeling, enzovoort. Wij leveren niet alleen producten, maar kunnen ook OEM uitvoeren, ODM, en andere behoeften. Als u gerelateerde behoeften heeft, Neem gerust contact met ons op via sales01@lingjunbio.com.