Lnjnbio Magnetic Beads for Animal Tissue Total RNA Extraction Kit 231002

(Méthode des billes magnétiques)

【Product Introduction】

This kit uses a unique separation magnetic bead and buffer system to extract RNA from animal tissues, and the whole process is easy to operate, which is especially suitable for automated nucleic acid extraction platforms. The extracted nucleic acids have high yield, high purity, stable and reliable quality, and can be applied to various downstream molecular biology experiments.

【Product advantages】

1. Easy and fast: Samples are directly lysed without liquid nitrogen grinding, and high-quality RNA can be obtained in less than 1 h without long incubation.
2. Safe and non-toxic: No organic reagents such as phenol/chloroform are required.
3. Wide range of applications: Suitable for a wide range of animal tissues, soft tissues (par ex., liver, kidney), adipose tissue (par ex., brain), and fibrous tissues (par ex., muscle), as well as more difficult samples such as mouse tails, shell tissues.
4. High-throughput: Magnetic stand manual extraction can be selected or nucleic acid extractor can be used to achieve automated, high-throughput operation.
5. Efficient extraction: Biomagnetic beads have a small size effect and surface effect, which can efficiently extract nucleic acids and meet the requirements of nucleic acid extraction from rare samples and trace biological samples.

【Product image】

 

【Experimental case】

RNA was extracted from different tissue samples from different animals, and the results were as follows:

Cas 1:

No.	Échantillon	A260/A280	A260/A230	Conc.(de/ul)
1	20mg Shrimp	1.863	1.725	65.48
2		1.865	1.782	68.96
Avg		1.864	1.7535	67.22
3	5mg Chicken heart	1.958	1.721	55.72
4		1.966	1.736	56.68
Avg		1.962	1.7285	56.2
5	5mg Fish heart	1.944	1.761	28.56
6		1.946	1.742	27.84
Avg		1.945	1.7515	28.2
7	5mg Fish liver	1.911	2.07	91.84
8		1.912	2.061	96.44
Avg		1.9115	2.0655	94.14
9	10mg Fish intestines	1.925	2.196	390.72
10		1.928	2.187	382.04
Avg		1.9265	2.1915	386.38

The electropherogram is shown below:

M.:Marker（2K，1K，750bp，500bp，250bp，100bp）

1-2：20 mg Shrimp 3-4：5 mg Chicken heart 5-6: 5mg Fish heart

7-8: 5mg Fish liver 9-10：10 mg Fish intestines

Result analysis:

The results of the Ultra-Micro Nucleic Acid Assay (UMNA) test showed that:

(1) The value of A260/A280 is 1.86-1.96, and the value of A260/A230 is above 1.75, which proves that the purity of the extracted product is high.   

(2) The yield of nucleic acid extraction from different animal tissue samples was more than 0.2 µg.

The results of electropherogram showed that:

(1) Different animal tissue samples had bright bands and good integrity.

En résumé, the automatic nucleic acid extraction and purification instrument was used to extract different animal tissue samples, and the extracted products had high purity, rendement élevé, complete electrophoresis bands.

Case2：

No.	Échantillon	A260/A280	A260/A230	Conc.(de/ul)
1	20mg Pork	1.937	1.478	32.64
2		1.946	1.473	32.76
Avg		1.9415	1.4755	32.7
3	20mg Beef	1.847	1.517	98.32
4		1.85	1.515	97.64
Avg		1.8485	1.516	97.98
5	20mgMutton	1.884	1.453	87.04
6		1.883	1.457	87.04
Avg		1.8835	1.455	87.04
7	20mgChicken	1.83	1.419	79.32
8		1.826	1.415	79.8
Avg		1.828	1.417	79.56

The electropherogram is shown below:

M.:Marker（2K，1K，750bp，500bp，250bp，100bp）;  

1-2： 20mg Pork 3-4：20mg Beef 5-6：20mg Mutton 7-8：20mgChicken

Result analysis:

The results of the Ultra-Micro Nucleic Acid Assay (UMNA) test showed that:

(1) The value of A260/A280 is 1.82-1.94, and the value of A260/A230 is above 1.4, which proves that the purity of the extracted product is high.

(2) The yield of nucleic acid extraction from different animal tissue samples was more than 2.6 µg.

The results of agarose gel electrophoresis showed that:

(1) Different animal tissue samples had bright bands and good integrity.

En résumé, the automatic nucleic acid extraction and purification instrument was used to extract different animal tissue samples, and the extracted products had high purity, rendement élevé, complete electrophoresis bands.

【Application area】

Basic molecular biology studies

It is suitable for extracting high-quality total RNA from complex animal tissues such as liver, brain, muscle and muscle for gene expression analysis (e. g., qRT-PCR, RNA-seq), transcriptomic studies and exploration of non-coding RNA function. It is especially suitable for processing samples rich in lipids or collagen, and magnetic bead-specific binding to RNA can effectively remove polysaccharides, proteins and other disruptors.

Disease mechanism and diagnostic studies

In oncology, neurodegenerative diseases, etc., they were used to extract RNA from pathological tissue samples to analyze disease-related gene expression profiles. Such as cancer studies detecting mRNA variants in cancerous tissues, or dysregulation of miRNA in neural tissues.

Drug development and safety evaluation

Supporting the assessment of the effect of drugs on gene expression in target organs in pharmacodynamic studies and the expression detection of genes related to drug metabolism (e. g., CYP450 family) in toxicology experiments.

Veterinary medicine and agricultural biotechnology

It is used for the diagnosis of livestock and poultry diseases (such as African swine fever virus detection), the verification of gene editing effect in the construction of transgenic animal models, and the functional analysis of genes related to the growth and development of economic animals.

Species identification

Combined with species-specific RNA markers, which can be used for source identification of biological samples. With easy operation (automation), high purity (A260 / A280> 1.9) and good integrity (RIN 8), this technology has become an important supporting platform for modern molecular experiments.

Présentation du fournisseur

Société de biotechnologie Shanghai Lingjun., Ltd.a été établi en 2016 and is a professional manufacturer of biomagnetic materials and nucleic acid extraction reagents.

Nous avons une riche expérience dans l'extraction et la purification des acides nucléiques, purification des protéines, séparation cellulaire, chimiluminescence et autres domaines techniques.

Nos produits sont largement utilisés dans de nombreux domaines, comme les tests médicaux, tests génétiques, recherche universitaire, sélection génétique, et ainsi de suite. Nous fournissons non seulement des produits, mais pouvons également entreprendre des OEM, ODM, et autres besoins. Si vous avez des besoins connexes, please feel free to contact us at sales01@lingjunbio.com.