Lnjnbio Coirníní Maighnéadacha le haghaidh Kit Eastóscadh Iomlán RNA Fíocháin Ainmhithe 231002

(Modh Coirnín Maighnéadach)

【Réamhrá Táirge】

This kit uses a unique separation magnetic bead and buffer system to extract RNA from animal tissues, and the whole process is easy to operate, which is especially suitable for automated nucleic acid extraction platforms. The extracted nucleic acids have high yield, íonachta ard, cáilíocht cobhsaí agus iontaofa, agus is féidir iad a chur i bhfeidhm ar thurgnaimh éagsúla bitheolaíochta mhóilíneacha iartheachtacha.

【Buntáistí táirge】

1. Easy and fast: Samples are directly lysed without liquid nitrogen grinding, and high-quality RNA can be obtained in less than 1 h without long incubation.
2. Sábháilte agus neamh-tocsaineach: No organic reagents such as phenol/chloroform are required.
3. Raon leathan iarratas: Suitable for a wide range of animal tissues, soft tissues (e.g., ae, kidney), adipose tissue (e.g., brain), and fibrous tissues (e.g., muscle), as well as more difficult samples such as mouse tails, shell tissues.
4. Ard-thréchur: Magnetic stand manual extraction can be selected or nucleic acid extractor can be used to achieve automated, oibríocht ard-thréchur.
5. Efficient extraction: Biomagnetic beads have a small size effect and surface effect, which can efficiently extract nucleic acids and meet the requirements of nucleic acid extraction from rare samples and trace biological samples.

【Íomhá táirge】

 

【Cás turgnamhach】

RNA was extracted from different tissue samples from different animals, agus bhí na torthaí mar seo a leanas:

Cás 1:

Níl.	Sampla	A260/A280	A260/A230	Conc.(de/ul)
1	20mg Shrimp	1.863	1.725	65.48
2		1.865	1.782	68.96
Meán		1.864	1.7535	67.22
3	5mg Croí sicín	1.958	1.721	55.72
4		1.966	1.736	56.68
Meán		1.962	1.7285	56.2
5	5mg Croí éisc	1.944	1.761	28.56
6		1.946	1.742	27.84
Meán		1.945	1.7515	28.2
7	5mg ae éisc	1.911	2.07	91.84
8		1.912	2.061	96.44
Meán		1.9115	2.0655	94.14
9	10mg intestines Éisc	1.925	2.196	390.72
10		1.928	2.187	382.04
Meán		1.9265	2.1915	386.38

Taispeántar an electropherogram thíos:

M:Marcóir （2K，1K，750bp，500bp，250bp，100bp）

1-2：20 mg Shrimp 3-4：5 mg Chicken heart 5-6: 5mg Croí éisc

7-8: 5mg Fish liver 9-10：10 mg intestines Éisc

Anailís ar thorthaí:

Torthaí an Mheasúnaithe Ultra-Micri-Aigéad Núicléach (UMNA) léirigh tástáil go:

(1) The value of A260/A280 is 1.86-1.96, and the value of A260/A230 is above 1.75, which proves that the purity of the extracted product is high.   

(2) The yield of nucleic acid extraction from different animal tissue samples was more than 0.2 μg.

The results of electropherogram showed that:

(1) Different animal tissue samples had bright bands and good integrity.

Go hachomair, the automatic nucleic acid extraction and purification instrument was used to extract different animal tissue samples, and the extracted products had high purity, toradh ard, complete electrophoresis bands.

Cás2：

Níl.	Sampla	A260/A280	A260/A230	Conc.(de/ul)
1	20mg Pork	1.937	1.478	32.64
2		1.946	1.473	32.76
Meán		1.9415	1.4755	32.7
3	20mg Beef	1.847	1.517	98.32
4		1.85	1.515	97.64
Meán		1.8485	1.516	97.98
5	20mgMutton	1.884	1.453	87.04
6		1.883	1.457	87.04
Meán		1.8835	1.455	87.04
7	20mgChicken	1.83	1.419	79.32
8		1.826	1.415	79.8
Meán		1.828	1.417	79.56

Taispeántar an electropherogram thíos:

M:Marcóir （2K，1K，750bp，500bp，250bp，100bp）;  

1-2： 20mg Pork 3-4：20mg Beef 5-6：20mg Mutton 7-8：20mgChicken

Anailís ar thorthaí:

Torthaí an Mheasúnaithe Ultra-Micri-Aigéad Núicléach (UMNA) léirigh tástáil go:

(1) The value of A260/A280 is 1.82-1.94, and the value of A260/A230 is above 1.4, which proves that the purity of the extracted product is high.

(2) The yield of nucleic acid extraction from different animal tissue samples was more than 2.6 μg.

Léirigh torthaí leictreafóiréis glóthach agarose go:

(1) Different animal tissue samples had bright bands and good integrity.

Go hachomair, the automatic nucleic acid extraction and purification instrument was used to extract different animal tissue samples, and the extracted products had high purity, toradh ard, complete electrophoresis bands.

【Limistéar iarratais】

Basic molecular biology studies

It is suitable for extracting high-quality total RNA from complex animal tissues such as liver, brain, muscle and muscle for gene expression analysis (e. g., qRT-PCR, RNA-seq), transcriptomic studies and exploration of non-coding RNA function. It is especially suitable for processing samples rich in lipids or collagen, and magnetic bead-specific binding to RNA can effectively remove polysaccharides, proteins and other disruptors.

Disease mechanism and diagnostic studies

In oncology, neurodegenerative diseases, srl., they were used to extract RNA from pathological tissue samples to analyze disease-related gene expression profiles. Such as cancer studies detecting mRNA variants in cancerous tissues, or dysregulation of miRNA in neural tissues.

Drug development and safety evaluation

Supporting the assessment of the effect of drugs on gene expression in target organs in pharmacodynamic studies and the expression detection of genes related to drug metabolism (e. g., CYP450 family) in toxicology experiments.

Veterinary medicine and agricultural biotechnology

It is used for the diagnosis of livestock and poultry diseases (such as African swine fever virus detection), the verification of gene editing effect in the construction of transgenic animal models, and the functional analysis of genes related to the growth and development of economic animals.

Species identification

Combined with species-specific RNA markers, which can be used for source identification of biological samples. With easy operation (uathoibriú), íonachta ard (A260 / A280> 1.9) and good integrity (RIN 8), this technology has become an important supporting platform for modern molecular experiments.

Réamhrá Soláthraí

Shanghai Lingjun Biteicneolaíocht Co., Teo.bunaíodh i 2016 agus is monaróir gairmiúil ábhair bhithmhaighnéadacha agus imoibrithe eastóscadh aigéid núicléacha.

Tá taithí shaibhir againn in eastóscadh agus íonú aigéad núicléasach, íonú próitéine, scaradh cille, chemiluminescence agus réimsí teicniúla eile.

Úsáidtear ár gcuid táirgí go forleathan i go leor réimsí, mar thástáil leighis, tástáil ghéiniteach, taighde ollscoile, pórú géiniteach, agus mar sin de. Ní hamháin go gcuirimid táirgí ar fáil ach is féidir linn OEM a dhéanamh freisin, ODM, agus riachtanais eile. Má tá riachtanais ghaolmhara agat, bíodh leisce ort teagmháil a dhéanamh linn ag sales01@lingjunbio.com.