Lnjnbio 磁気ビーズ動物組織用トータル RNA 抽出キット 231002

(磁気ビーズ法)

【商品紹介】

This kit uses a unique separation magnetic bead and buffer system to extract RNA from animal tissues, and the whole process is easy to operate, which is especially suitable for automated nucleic acid extraction platforms. The extracted nucleic acids have high yield, 高純度, 安定した信頼できる品質, さまざまな下流の分子生物学実験に適用できます.

【製品の利点】

1. Easy and fast: Samples are directly lysed without liquid nitrogen grinding, and high-quality RNA can be obtained in less than 1 h without long incubation.
2. 安全で無毒: No organic reagents such as phenol/chloroform are required.
3. Wide range of applications: Suitable for a wide range of animal tissues, soft tissues (例えば, 肝臓, kidney), adipose tissue (例えば, brain), and fibrous tissues (例えば, muscle), as well as more difficult samples such as mouse tails, shell tissues.
4. High-throughput: Magnetic stand manual extraction can be selected or nucleic acid extractor can be used to achieve automated, 高スループット動作.
5. Efficient extraction: Biomagnetic beads have a small size effect and surface effect, which can efficiently extract nucleic acids and meet the requirements of nucleic acid extraction from rare samples and trace biological samples.

【商品画像】

 

【実験事例】

RNA was extracted from different tissue samples from different animals, そして結果は次のとおりでした:

場合 1:

いいえ.	サンプル	A260/A280	A260/A230	濃度(の/ul)
1	20mg エビ	1.863	1.725	65.48
2		1.865	1.782	68.96
平均		1.864	1.7535	67.22
3	5mg 鶏ハツ	1.958	1.721	55.72
4		1.966	1.736	56.68
平均		1.962	1.7285	56.2
5	5mg 魚の心臓	1.944	1.761	28.56
6		1.946	1.742	27.84
平均		1.945	1.7515	28.2
7	5mg 魚の肝臓	1.911	2.07	91.84
8		1.912	2.061	96.44
平均		1.9115	2.0655	94.14
9	10mg 魚の腸	1.925	2.196	390.72
10		1.928	2.187	382.04
平均		1.9265	2.1915	386.38

エレクトロフェログラムを以下に示します:

M:マーカー（2K），1K，750血圧，500血圧，250血圧，100血圧）

1-2：20 mg Shrimp 3-4：5 mg Chicken heart 5-6: 5mg 魚の心臓

7-8: 5mg Fish liver 9-10：10 mg 魚の腸

結果分析:

超微量核酸測定の結果 (ウムナ) テストでそれが示された:

(1) The value of A260/A280 is 1.86-1.96, and the value of A260/A230 is above 1.75, which proves that the purity of the extracted product is high.   

(2) The yield of nucleic acid extraction from different animal tissue samples was more than 0.2 μg.

The results of electropherogram showed that:

(1) Different animal tissue samples had bright bands and good integrity.

要約すれば, the automatic nucleic acid extraction and purification instrument was used to extract different animal tissue samples, 抽出物は高純度でした, 高収量, 完全な電気泳動バンド.

事例2：

いいえ.	サンプル	A260/A280	A260/A230	濃度(の/ul)
1	20mg 豚肉	1.937	1.478	32.64
2		1.946	1.473	32.76
平均		1.9415	1.4755	32.7
3	20mg 牛肉	1.847	1.517	98.32
4		1.85	1.515	97.64
平均		1.8485	1.516	97.98
5	20mgMutton	1.884	1.453	87.04
6		1.883	1.457	87.04
平均		1.8835	1.455	87.04
7	20mgChicken	1.83	1.419	79.32
8		1.826	1.415	79.8
平均		1.828	1.417	79.56

エレクトロフェログラムを以下に示します:

M:マーカー（2K），1K，750血圧，500血圧，250血圧，100血圧）;  

1-2： 20mg Pork 3-4：20mg Beef 5-6：20mg Mutton 7-8：20mgChicken

結果分析:

超微量核酸測定の結果 (ウムナ) テストでそれが示された:

(1) The value of A260/A280 is 1.82-1.94, A260/A230の値が上である 1.4, 抽出物の純度が高いことを証明しています.

(2) The yield of nucleic acid extraction from different animal tissue samples was more than 2.6 μg.

アガロースゲル電気泳動の結果、:

(1) Different animal tissue samples had bright bands and good integrity.

要約すれば, the automatic nucleic acid extraction and purification instrument was used to extract different animal tissue samples, 抽出物は高純度でした, 高収量, 完全な電気泳動バンド.

【適用範囲】

Basic molecular biology studies

It is suitable for extracting high-quality total RNA from complex animal tissues such as liver, brain, muscle and muscle for gene expression analysis (e. g., qRT-PCR, RNA配列), transcriptomic studies and exploration of non-coding RNA function. It is especially suitable for processing samples rich in lipids or collagen, and magnetic bead-specific binding to RNA can effectively remove polysaccharides, proteins and other disruptors.

Disease mechanism and diagnostic studies

In oncology, neurodegenerative diseases, 等, they were used to extract RNA from pathological tissue samples to analyze disease-related gene expression profiles. Such as cancer studies detecting mRNA variants in cancerous tissues, or dysregulation of miRNA in neural tissues.

Drug development and safety evaluation

Supporting the assessment of the effect of drugs on gene expression in target organs in pharmacodynamic studies and the expression detection of genes related to drug metabolism (e. g., CYP450 family) in toxicology experiments.

Veterinary medicine and agricultural biotechnology

It is used for the diagnosis of livestock and poultry diseases (such as African swine fever virus detection), the verification of gene editing effect in the construction of transgenic animal models, and the functional analysis of genes related to the growth and development of economic animals.

Species identification

Combined with species-specific RNA markers, which can be used for source identification of biological samples. With easy operation (オートメーション), 高純度 (A260 / A280> 1.9) and good integrity (リン 8), this technology has become an important supporting platform for modern molecular experiments.

サプライヤー紹介

上海霊軍生物技術有限公司, 株式会社に設立されました 2016 生体磁性材料と核酸抽出試薬の専門メーカーです.

核酸抽出・精製の経験が豊富です。, タンパク質の精製, 細胞分離, 化学発光およびその他の技術分野.

当社の製品はさまざまな分野で広く使用されています, 医療検査など, 遺伝子検査, 大学の研究, 遺伝子育種, 等々. 製品のご提供だけでなく、OEMも承ります。, ODM, その他のニーズ. 関連するニーズがある場合, sales01@lingjunbio.comまでお気軽にお問い合わせください。.