Lnjnbio Magnetic Beads for Animal Tissue Total RNA Extraction Kit 231002

(चुंबकीय मनका विधि)

【उत्पाद परिचय】

This kit uses a unique separation magnetic bead and buffer system to extract RNA from animal tissues, and the whole process is easy to operate, which is especially suitable for automated nucleic acid extraction platforms. The extracted nucleic acids have high yield, उच्च शुद्धता, स्थिर और विश्वसनीय गुणवत्ता, और इसे विभिन्न डाउनस्ट्रीम आणविक जीवविज्ञान प्रयोगों पर लागू किया जा सकता है.

【उत्पाद लाभ】

1. Easy and fast: Samples are directly lysed without liquid nitrogen grinding, and high-quality RNA can be obtained in less than 1 h without long incubation.
2. सुरक्षित और गैर विषैले: No organic reagents such as phenol/chloroform are required.
3. अनुप्रयोगों की विस्तृत श्रृंखला: Suitable for a wide range of animal tissues, soft tissues (जैसे, liver, kidney), adipose tissue (जैसे, brain), and fibrous tissues (जैसे, muscle), as well as more difficult samples such as mouse tails, shell tissues.
4. उच्च throughput: Magnetic stand manual extraction can be selected or nucleic acid extractor can be used to achieve automated, high-throughput operation.
5. Efficient extraction: Biomagnetic beads have a small size effect and surface effect, which can efficiently extract nucleic acids and meet the requirements of nucleic acid extraction from rare samples and trace biological samples.

【उत्पाद छवि】

 

【प्रायोगिक मामला】

RNA was extracted from different tissue samples from different animals, and the results were as follows:

मामला 1:

No.	नमूना	ए260/ए280	ए260/ए230	संक्षिप्त.(ng/ul)
1	20mg Shrimp	1.863	1.725	65.48
2		1.865	1.782	68.96
Avg		1.864	1.7535	67.22
3	5एमजी चिकन दिल	1.958	1.721	55.72
4		1.966	1.736	56.68
Avg		1.962	1.7285	56.2
5	5mg Fish heart	1.944	1.761	28.56
6		1.946	1.742	27.84
Avg		1.945	1.7515	28.2
7	5एमजी मछली का जिगर	1.911	2.07	91.84
8		1.912	2.061	96.44
Avg		1.9115	2.0655	94.14
9	10mg Fish intestines	1.925	2.196	390.72
10		1.928	2.187	382.04
Avg		1.9265	2.1915	386.38

इलेक्ट्रोफेरोग्राम नीचे दिखाया गया है:

M:Marker（2K，1K，750bp，500bp，250bp，100bp）

1-2：20 mg Shrimp 3-4：5 mg Chicken heart 5-6: 5mg Fish heart

7-8: 5mg Fish liver 9-10：10 mg Fish intestines

परिणाम विश्लेषण:

अल्ट्रा-माइक्रो न्यूक्लिक एसिड परख के परिणाम (UMNA) परीक्षण से यह पता चला:

(1) The value of A260/A280 is 1.86-1.96, and the value of A260/A230 is above 1.75, which proves that the purity of the extracted product is high.   

(2) The yield of nucleic acid extraction from different animal tissue samples was more than 0.2 माइक्रोग्राम.

The results of electropherogram showed that:

(1) Different animal tissue samples had bright bands and good integrity.

सारांश, the automatic nucleic acid extraction and purification instrument was used to extract different animal tissue samples, and the extracted products had high purity, उच्च उपज, complete electrophoresis bands.

Case2：

No.	नमूना	ए260/ए280	ए260/ए230	संक्षिप्त.(ng/ul)
1	20mg Pork	1.937	1.478	32.64
2		1.946	1.473	32.76
Avg		1.9415	1.4755	32.7
3	20mg Beef	1.847	1.517	98.32
4		1.85	1.515	97.64
Avg		1.8485	1.516	97.98
5	20mgMutton	1.884	1.453	87.04
6		1.883	1.457	87.04
Avg		1.8835	1.455	87.04
7	20mgChicken	1.83	1.419	79.32
8		1.826	1.415	79.8
Avg		1.828	1.417	79.56

इलेक्ट्रोफेरोग्राम नीचे दिखाया गया है:

M:Marker（2K，1K，750bp，500bp，250bp，100bp）;  

1-2： 20mg Pork 3-4：20mg Beef 5-6：20mg Mutton 7-8：20mgChicken

परिणाम विश्लेषण:

अल्ट्रा-माइक्रो न्यूक्लिक एसिड परख के परिणाम (UMNA) परीक्षण से यह पता चला:

(1) The value of A260/A280 is 1.82-1.94, and the value of A260/A230 is above 1.4, which proves that the purity of the extracted product is high.

(2) The yield of nucleic acid extraction from different animal tissue samples was more than 2.6 माइक्रोग्राम.

एगरोज़ जेल वैद्युतकणसंचलन के परिणामों से पता चला है:

(1) Different animal tissue samples had bright bands and good integrity.

सारांश, the automatic nucleic acid extraction and purification instrument was used to extract different animal tissue samples, and the extracted products had high purity, उच्च उपज, complete electrophoresis bands.

【आवेदन क्षेत्र】

Basic molecular biology studies

It is suitable for extracting high-quality total RNA from complex animal tissues such as liver, brain, muscle and muscle for gene expression analysis (ई. g., qRT-PCR, RNA-seq), transcriptomic studies and exploration of non-coding RNA function. It is especially suitable for processing samples rich in lipids or collagen, and magnetic bead-specific binding to RNA can effectively remove polysaccharides, proteins and other disruptors.

Disease mechanism and diagnostic studies

In oncology, neurodegenerative diseases, वगैरह।, they were used to extract RNA from pathological tissue samples to analyze disease-related gene expression profiles. Such as cancer studies detecting mRNA variants in cancerous tissues, or dysregulation of miRNA in neural tissues.

Drug development and safety evaluation

Supporting the assessment of the effect of drugs on gene expression in target organs in pharmacodynamic studies and the expression detection of genes related to drug metabolism (ई. g., CYP450 family) in toxicology experiments.

Veterinary medicine and agricultural biotechnology

It is used for the diagnosis of livestock and poultry diseases (such as African swine fever virus detection), the verification of gene editing effect in the construction of transgenic animal models, and the functional analysis of genes related to the growth and development of economic animals.

Species identification

Combined with species-specific RNA markers, which can be used for source identification of biological samples. With easy operation (automation), उच्च शुद्धता (A260 / A280> 1.9) and good integrity (RIN 8), this technology has become an important supporting platform for modern molecular experiments.

आपूर्तिकर्ता परिचय

शंघाई लिंगजुन बायोटेक्नोलॉजी कंपनी, लिमिटेडमें स्थापित किया गया था 2016 और जैवचुंबकीय सामग्री और न्यूक्लिक एसिड निष्कर्षण अभिकर्मकों का एक पेशेवर निर्माता है.

हमारे पास न्यूक्लिक एसिड निष्कर्षण और शुद्धिकरण में समृद्ध अनुभव है, प्रोटीन शुद्धि, कोशिका पृथक्करण, केमिलुमिनसेंस और अन्य तकनीकी क्षेत्र.

हमारे उत्पाद कई क्षेत्रों में व्यापक रूप से उपयोग किए जाते हैं, जैसे चिकित्सा परीक्षण, आनुवंशिक परीक्षण, विश्वविद्यालय अनुसंधान, आनुवंशिक प्रजनन, और इसी तरह. हम न केवल उत्पाद उपलब्ध कराते हैं बल्कि ओईएम का कार्य भी कर सकते हैं, ओडीएम, और अन्य जरूरतें. यदि आपके पास संबंधित आवश्यकताएं हैं, कृपया बेझिझक हमसे sales01@lingjunbio.com पर संपर्क करें.