Manik Magnetik Lnjnbio untuk Kit Pengekstrakan Jumlah RNA Tisu Haiwan 231002

(Kaedah Manik Magnet)

【Pengenalan Produk】

This kit uses a unique separation magnetic bead and buffer system to extract RNA from animal tissues, and the whole process is easy to operate, which is especially suitable for automated nucleic acid extraction platforms. The extracted nucleic acids have high yield, kesucian yang tinggi, kualiti yang stabil dan boleh dipercayai, dan boleh digunakan untuk pelbagai eksperimen biologi molekul hiliran.

【Kelebihan produk】

1. Easy and fast: Samples are directly lysed without liquid nitrogen grinding, and high-quality RNA can be obtained in less than 1 h without long incubation.
2. Selamat dan tidak toksik: No organic reagents such as phenol/chloroform are required.
3. Pelbagai aplikasi: Suitable for a wide range of animal tissues, soft tissues (cth., liver, kidney), adipose tissue (cth., brain), and fibrous tissues (cth., muscle), as well as more difficult samples such as mouse tails, shell tissues.
4. Kapasiti tinggi: Magnetic stand manual extraction can be selected or nucleic acid extractor can be used to achieve automated, operasi pemprosesan tinggi.
5. Efficient extraction: Biomagnetic beads have a small size effect and surface effect, which can efficiently extract nucleic acids and meet the requirements of nucleic acid extraction from rare samples and trace biological samples.

【Imej produk】

 

【Kes eksperimen】

RNA was extracted from different tissue samples from different animals, dan hasilnya adalah seperti berikut:

Kes 1:

Tidak.	Sampel	A260/A280	A260/A230	Conc.(daripada/ul)
1	20mg Udang	1.863	1.725	65.48
2		1.865	1.782	68.96
Purata		1.864	1.7535	67.22
3	5mg Jantung ayam	1.958	1.721	55.72
4		1.966	1.736	56.68
Purata		1.962	1.7285	56.2
5	5mg Jantung ikan	1.944	1.761	28.56
6		1.946	1.742	27.84
Purata		1.945	1.7515	28.2
7	5mg Hati ikan	1.911	2.07	91.84
8		1.912	2.061	96.44
Purata		1.9115	2.0655	94.14
9	10mg Usus ikan	1.925	2.196	390.72
10		1.928	2.187	382.04
Purata		1.9265	2.1915	386.38

Elektroferogram ditunjukkan di bawah:

M:Penanda（2K，1K，750bp，500bp，250bp，100bp）

1-2：20 mg Shrimp 3-4：5 mg Chicken heart 5-6: 5mg Jantung ikan

7-8: 5mg Fish liver 9-10：10 mg Usus ikan

Analisis keputusan:

Keputusan Ujian Asid Nukleik Ultra-Mikro (UMNA) ujian menunjukkan bahawa:

(1) The value of A260/A280 is 1.86-1.96, and the value of A260/A230 is above 1.75, which proves that the purity of the extracted product is high.   

(2) The yield of nucleic acid extraction from different animal tissue samples was more than 0.2 μg.

The results of electropherogram showed that:

(1) Different animal tissue samples had bright bands and good integrity.

Secara ringkasnya, the automatic nucleic acid extraction and purification instrument was used to extract different animal tissue samples, and the extracted products had high purity, hasil yang tinggi, jalur elektroforesis lengkap.

Case2：

Tidak.	Sampel	A260/A280	A260/A230	Conc.(daripada/ul)
1	20mg Pork	1.937	1.478	32.64
2		1.946	1.473	32.76
Purata		1.9415	1.4755	32.7
3	20mg Beef	1.847	1.517	98.32
4		1.85	1.515	97.64
Purata		1.8485	1.516	97.98
5	20mgMutton	1.884	1.453	87.04
6		1.883	1.457	87.04
Purata		1.8835	1.455	87.04
7	20mgChicken	1.83	1.419	79.32
8		1.826	1.415	79.8
Purata		1.828	1.417	79.56

Elektroferogram ditunjukkan di bawah:

M:Penanda（2K，1K，750bp，500bp，250bp，100bp）;  

1-2： 20mg Pork 3-4：20mg Beef 5-6：20mg Mutton 7-8：20mgChicken

Analisis keputusan:

Keputusan Ujian Asid Nukleik Ultra-Mikro (UMNA) ujian menunjukkan bahawa:

(1) The value of A260/A280 is 1.82-1.94, and the value of A260/A230 is above 1.4, which proves that the purity of the extracted product is high.

(2) The yield of nucleic acid extraction from different animal tissue samples was more than 2.6 μg.

Keputusan elektroforesis gel agarose menunjukkan bahawa:

(1) Different animal tissue samples had bright bands and good integrity.

Secara ringkasnya, the automatic nucleic acid extraction and purification instrument was used to extract different animal tissue samples, and the extracted products had high purity, hasil yang tinggi, jalur elektroforesis lengkap.

【Kawasan permohonan】

Basic molecular biology studies

It is suitable for extracting high-quality total RNA from complex animal tissues such as liver, brain, muscle and muscle for gene expression analysis (e. g., qRT-PCR, RNA-seq), transcriptomic studies and exploration of non-coding RNA function. It is especially suitable for processing samples rich in lipids or collagen, and magnetic bead-specific binding to RNA can effectively remove polysaccharides, proteins and other disruptors.

Disease mechanism and diagnostic studies

In oncology, neurodegenerative diseases, dll., they were used to extract RNA from pathological tissue samples to analyze disease-related gene expression profiles. Such as cancer studies detecting mRNA variants in cancerous tissues, or dysregulation of miRNA in neural tissues.

Drug development and safety evaluation

Supporting the assessment of the effect of drugs on gene expression in target organs in pharmacodynamic studies and the expression detection of genes related to drug metabolism (e. g., CYP450 family) in toxicology experiments.

Veterinary medicine and agricultural biotechnology

It is used for the diagnosis of livestock and poultry diseases (such as African swine fever virus detection), the verification of gene editing effect in the construction of transgenic animal models, and the functional analysis of genes related to the growth and development of economic animals.

Species identification

Combined with species-specific RNA markers, which can be used for source identification of biological samples. With easy operation (automasi), kesucian yang tinggi (A260 / A280> 1.9) and good integrity (RIN 8), this technology has become an important supporting platform for modern molecular experiments.

Pengenalan Pembekal

Shanghai Lingjun Biotechnology Co., Ltd.ditubuhkan pada 2016 dan merupakan pengilang profesional bahan biomagnet dan reagen pengekstrakan asid nukleik.

Kami mempunyai pengalaman yang kaya dalam pengekstrakan dan penulenan asid nukleik, penulenan protein, pemisahan sel, chemiluminescence dan bidang teknikal lain.

Produk kami digunakan secara meluas dalam pelbagai bidang, seperti ujian perubatan, ujian genetik, penyelidikan universiti, pembiakan genetik, dan seterusnya. Kami bukan sahaja menyediakan produk tetapi juga boleh menjalankan OEM, ODM, dan keperluan lain. Jika anda mempunyai keperluan yang berkaitan, sila hubungi kami di sales01@lingjunbio.com.