Manik Magnetik Lnjnbio untuk Polisakarida dan Polifenol Kit Pengekstrakan Jumlah RNA Tumbuhan231001

(Kaedah Manik Magnet)

【Pengenalan Produk】

This kit uses magnetic beads and buffer system with unique separation effect to extract total RNA from roots, batang, leaves and seeds of polysaccharide and polyphenol plants, and the whole process is easy to operate especially suitable for automated nucleic acid extraction platform. The total RNA extracted is of high yield, kesucian yang tinggi, kualiti yang stabil dan boleh dipercayai, dan boleh digunakan untuk pelbagai eksperimen biologi molekul hiliran.

【Kelebihan produk】

Magnetic bead-based extraction eliminates the need for repeated centrifugation, ensuring the integrity of the extracted total RNA.

2. The extracted product is of high purity and yield, with complete electrophoresis bands, and can be directly used for various downstream applications such as high-throughput sequencing, RT-PCR, analisis microarray, and Northern Blot.

3. The process is safe to use, without the need for liquid nitrogen grinding, and the product does not contain toxic reagents such as phenol or chloroform.

4. Simple and rapid operation: The entire extraction process is time-efficient and does not involve toxic organic solvents like phenol or chloroform, making it safe and environmentally friendly.

5. Wide sample compatibility: Suitable for a variety of plant samples, including perilla leaves, mint leaves, and dandelion.

6. Keserasian automasi: It can be perfectly integrated with multiple automated nucleic acid extraction instruments to achieve high-throughput automated extraction.

【Imej produk】

【Kes eksperimen】

Kes 1:Sampel daun tumbuhan polifenol polisakarida yang berbeza telah diekstrak untuk jumlah RNA dengan keputusan berikut:

Tidak.	Sampel	A260/A280	A260/A230	Conc.(ng/µL)	Hasil（µg）
1	10mg daun perilla	1.964	1.641	71.92	7.192
2		1.965	1.653	71.92	7.192
Purata		1.9645	1.647	71.92	7.192
3	10mg Daun pudina	1.967	2.124	149.72	14.972
4		1.961	2.158	149.76	14.976
Purata		1.964	2.141	149.74	14.974
5	10mg daun dandelion	1.951	2.232	219.16	21.916
6		1.959	2.228	220.4	22.04
Purata		1.955	2.23	219.78	21.978

Gambar rajah corak puncak:

daun perilla:

Daun pudina:

Daun dandelion:

Jumlah rajah puncak:

Elektroferogram ditunjukkan di bawah:

 

Analisis keputusan:

Keputusan Ujian Asid Nukleik Ultra-Mikro (UMNA) ujian menunjukkan bahawa:

Keputusan ujian asid nukleik ultra-mikro menunjukkan bahawa:

① Nilai A260/A280 berada dalam julat 1.9~2.0, dan nilai A260/A230 adalah di atas 1.6, dan bentuk puncak adalah lengkap dan pada asasnya bertindih, yang membuktikan bahawa produk yang diekstrak mempunyai ketulenan yang tinggi.

②Hasil asid nukleik yang diekstrak daripada daun tumbuhan berbeza adalah di atas 7 µg.

Keputusan elektroforesis gel agarose menunjukkan bahawa:

① Semua sampel daun tumbuhan polisakarida polifenol yang berbeza mempunyai jalur terang dan integriti yang baik.

Kesimpulannya, produk yang diekstrak daripada sampel daun tumbuhan polifenol polisakarida yang berbeza adalah mempunyai ketulenan yang tinggi, hasil tinggi dan jalur elektroforesis lengkap.

【Akawasan aplikasi】

The RNA extracted by this kit can be applied to the following research fields:

1. Penyelidikan Sains Tumbuhan

Plant Gene Expression Studies: It can be used to analyze changes in gene expression at different growth stages or under stress conditions.

Plant Breeding: By extracting and analyzing plant RNA, genes related to desirable traits can be identified, providing theoretical support for plant breeding.

Plant Growth and Development Studies: RNA extracted from different plant tissues (such as roots, batang, daun, bunga, and fruits) can be used to study the genetic regulatory mechanisms during growth and development.

Stress Biology Research: It can be used to analyze changes in gene expression under adverse conditions such as drought, kemasinan, and high temperature.

• Analisis Ekspresi Gen

Real-time Quantitative PCR (qRT-PCR): The extracted RNA can be used for quantitative analysis of specific gene expression levels.

RNA Sequencing (RNA-seq): High-quality RNA is essential for transcriptome sequencing and can be used for comprehensive analysis of plant transcriptomes.

Microarray Analysis: The extracted RNA can be used for microarray analysis to study gene expression profiles.

• Eksperimen Biologi Molekul

Northern Blot and Dot Blot: These techniques are used to detect specific RNA bands or dot arrays.

PolyA Selection: It is used for enriching and studying mRNA.

In Vitro Translation: The extracted RNA can be used for in vitro translation experiments to study protein synthesis.

RNase Protection Assay: It is used to analyze RNA stability.

Molecular Cloning: The extracted RNA can be used for constructing cDNA libraries.

• High-Throughput Automated Extraction

Compatibility with Automated Extraction Devices: The magnetic bead-based extraction reagents are suitable for use with automated nucleic acid extraction instruments, allowing for rapid and efficient processing of large sample volumes.

High-Throughput Experiments: It is suitable for large-scale RNA extraction from plant samples, improving experimental efficiency.

• Aplikasi Lain

Penyelidikan RNA bukan pengekodan: The extracted RNA can be used to study the functions of non-coding RNAs such as miRNA and lncRNA.

Protein Synthesis and Modification Studies: By analyzing mRNA modifications (seperti metilasi m6A), the impact on translation efficiency can be studied.

Environmental Microbiology: It can be used to study the RNA of microbial communities in the rhizosphere of plants.

Pengenalan Pembekal

Shanghai Lingjun Biotechnology Co., Ltd.ditubuhkan pada 2016 dan merupakan pengilang profesional bahan biomagnet dan reagen pengekstrakan asid nukleik.

Kami mempunyai pengalaman yang kaya dalam pengekstrakan dan penulenan asid nukleik, penulenan protein, pemisahan sel, chemiluminescence dan bidang teknikal lain.

Produk kami digunakan secara meluas dalam pelbagai bidang, seperti ujian perubatan, ujian genetik, penyelidikan universiti, pembiakan genetik, dan seterusnya. Kami bukan sahaja menyediakan produk tetapi juga boleh menjalankan OEM, ODM, dan keperluan lain. Jika anda mempunyai keperluan yang berkaitan, sila hubungi kami di sales01@lingjunbio.com.