Lnjnbio 다당류 및 폴리페놀용 자성 비드 식물 총 RNA 추출 키트231001

(마그네틱 비드 방식)

【제품소개】

This kit uses magnetic beads and buffer system with unique separation effect to extract total RNA from roots, 줄기, leaves and seeds of polysaccharide and polyphenol plants, and the whole process is easy to operate especially suitable for automated nucleic acid extraction platform. The total RNA extracted is of high yield, 고순도, 안정적이고 믿을 수 있는 품질, and can be applied to various downstream molecular biology experiments.

【제품의 장점】

Magnetic bead-based extraction eliminates the need for repeated centrifugation, ensuring the integrity of the extracted total RNA.

2. The extracted product is of high purity and yield, with complete electrophoresis bands, and can be directly used for various downstream applications such as high-throughput sequencing, RT-PCR, 마이크로어레이 분석, and Northern Blot.

3. The process is safe to use, without the need for liquid nitrogen grinding, and the product does not contain toxic reagents such as phenol or chloroform.

4. Simple and rapid operation: The entire extraction process is time-efficient and does not involve toxic organic solvents like phenol or chloroform, making it safe and environmentally friendly.

5. Wide sample compatibility: Suitable for a variety of plant samples, including perilla leaves, mint leaves, and dandelion.

6. Automation compatibility: It can be perfectly integrated with multiple automated nucleic acid extraction instruments to achieve high-throughput automated extraction.

【상품 이미지】

【실험사례】

사례 1:다양한 다당류 폴리페놀 식물의 잎 샘플을 총 RNA에 대해 추출하여 다음과 같은 결과를 얻었습니다.:

아니요.	견본	A260/A280	A260/A230	농도(ng/μL)	수율(μg)
1	10mg 들깨 잎	1.964	1.641	71.92	7.192
2		1.965	1.653	71.92	7.192
평균		1.9645	1.647	71.92	7.192
3	10mg 민트 잎	1.967	2.124	149.72	14.972
4		1.961	2.158	149.76	14.976
평균		1.964	2.141	149.74	14.974
5	10mg 민들레 잎	1.951	2.232	219.16	21.916
6		1.959	2.228	220.4	22.04
평균		1.955	2.23	219.78	21.978

피크 패턴 다이어그램:

들깨잎:

민트 잎:

민들레 잎:

총 피크 다이어그램:

전기영동도는 아래와 같습니다.:

 

결과 분석:

초미세핵산 분석 결과 (움나) 테스트 결과:

초미세핵산 분석 결과는:

① A260/A280의 값이 1.9~2.0 범위에 있었습니다., A260/A230의 값은 위였습니다. 1.6, 피크 모양은 완전하고 기본적으로 겹쳐졌습니다., 추출된 제품의 순도가 높다는 것을 입증한 것입니다..

②다른 식물의 잎에서 추출한 핵산의 수율은 7 µg.

아가로스겔 전기영동 결과는:

① 다양한 다당류 폴리페놀 식물 잎의 모든 샘플은 밝은 밴드와 양호한 무결성을 가집니다..

결론적으로, 다양한 다당류 폴리페놀 식물 잎 샘플에서 추출된 제품의 순도가 높았습니다., 높은 수율과 완전한 전기영동 밴드.

【에이적용분야】

The RNA extracted by this kit can be applied to the following research fields:

1. 식물 과학 연구

Plant Gene Expression Studies: It can be used to analyze changes in gene expression at different growth stages or under stress conditions.

Plant Breeding: By extracting and analyzing plant RNA, genes related to desirable traits can be identified, providing theoretical support for plant breeding.

Plant Growth and Development Studies: RNA extracted from different plant tissues (such as roots, 줄기, 나뭇잎, flowers, and fruits) can be used to study the genetic regulatory mechanisms during growth and development.

Stress Biology Research: It can be used to analyze changes in gene expression under adverse conditions such as drought, 염분, and high temperature.

• Gene Expression Analysis

Real-time Quantitative PCR (qRT-PCR): The extracted RNA can be used for quantitative analysis of specific gene expression levels.

RNA Sequencing (RNA-seq): High-quality RNA is essential for transcriptome sequencing and can be used for comprehensive analysis of plant transcriptomes.

Microarray Analysis: The extracted RNA can be used for microarray analysis to study gene expression profiles.

• Molecular Biology Experiments

Northern Blot and Dot Blot: These techniques are used to detect specific RNA bands or dot arrays.

PolyA Selection: It is used for enriching and studying mRNA.

In Vitro Translation: The extracted RNA can be used for in vitro translation experiments to study protein synthesis.

RNase Protection Assay: It is used to analyze RNA stability.

Molecular Cloning: The extracted RNA can be used for constructing cDNA libraries.

• High-Throughput Automated Extraction

Compatibility with Automated Extraction Devices: The magnetic bead-based extraction reagents are suitable for use with automated nucleic acid extraction instruments, allowing for rapid and efficient processing of large sample volumes.

High-Throughput Experiments: It is suitable for large-scale RNA extraction from plant samples, improving experimental efficiency.

• Other Applications

Non-coding RNA Research: The extracted RNA can be used to study the functions of non-coding RNAs such as miRNA and lncRNA.

Protein Synthesis and Modification Studies: By analyzing mRNA modifications (such as m6A methylation), the impact on translation efficiency can be studied.

Environmental Microbiology: It can be used to study the RNA of microbial communities in the rhizosphere of plants.

공급업체 소개

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