Lnjnbio Perline magnetiche per polisaccaridi e polifenoli Kit di estrazione RNA totale vegetale231001

(Metodo della perla magnetica)

【Introduzione al prodotto】

This kit uses magnetic beads and buffer system with unique separation effect to extract total RNA from roots, steli, leaves and seeds of polysaccharide and polyphenol plants, and the whole process is easy to operate especially suitable for automated nucleic acid extraction platform. The total RNA extracted is of high yield, elevata purezza, qualità stabile e affidabile, e può essere applicato a vari esperimenti di biologia molecolare a valle.

【Vantaggi del prodotto】

Magnetic bead-based extraction eliminates the need for repeated centrifugation, ensuring the integrity of the extracted total RNA.

2. The extracted product is of high purity and yield, with complete electrophoresis bands, and can be directly used for various downstream applications such as high-throughput sequencing, RT-PCR, microarray analysis, and Northern Blot.

3. The process is safe to use, without the need for liquid nitrogen grinding, and the product does not contain toxic reagents such as phenol or chloroform.

4. Simple and rapid operation: The entire extraction process is time-efficient and does not involve toxic organic solvents like phenol or chloroform, making it safe and environmentally friendly.

5. Wide sample compatibility: Suitable for a variety of plant samples, including perilla leaves, mint leaves, and dandelion.

6. Automation compatibility: It can be perfectly integrated with multiple automated nucleic acid extraction instruments to achieve high-throughput automated extraction.

【Immagine del prodotto】

【Caso sperimentale】

Caso 1:Leaf samples of different polysaccharide polyphenol plants were extracted for total RNA with the following results:

No.	Campione	A260/A280	A260/A230	Concentrato(ng/μL)	Yield（µg）
1	10mg Perilla leaves	1.964	1.641	71.92	7.192
2		1.965	1.653	71.92	7.192
Media		1.9645	1.647	71.92	7.192
3	10mg Mint leaves	1.967	2.124	149.72	14.972
4		1.961	2.158	149.76	14.976
Media		1.964	2.141	149.74	14.974
5	10mg Dandelion leaves	1.951	2.232	219.16	21.916
6		1.959	2.228	220.4	22.04
Media		1.955	2.23	219.78	21.978

Diagramma del modello di picco:

Perilla leaves:

Mint leaves:

Dandelion leaves:

Diagramma del picco totale:

L'elettroferogramma è mostrato di seguito:

 

Analisi dei risultati:

I risultati del test dell'acido nucleico ultramicro (UMNA) il test lo ha dimostrato:

The results of the ultra-micro nucleic acid assay showed that:

① The values of A260/A280 were in the range of 1.9~2.0, e i valori di A260/A230 erano superiori 1.6, and the peak shapes were complete and basically overlapped, che ha dimostrato che i prodotti estratti erano di elevata purezza.

②The yields of nucleic acids extracted from the leaves of different plants were above 7 mg.

I risultati dell’elettroforesi su gel di agarosio lo hanno dimostrato:

① All the samples of different polysaccharide polyphenol plant leaves have bright bands and good integrity.

Insomma, the extracted products of different polysaccharide polyphenol plant leaf samples were of high purity, bande elettroforetiche complete e ad alta resa.

【UNarea di applicazione】

The RNA extracted by this kit can be applied to the following research fields:

1. Ricerca sulla scienza delle piante

Plant Gene Expression Studies: It can be used to analyze changes in gene expression at different growth stages or under stress conditions.

Plant Breeding: By extracting and analyzing plant RNA, genes related to desirable traits can be identified, providing theoretical support for plant breeding.

Plant Growth and Development Studies: RNA extracted from different plant tissues (such as roots, steli, foglie, flowers, and fruits) can be used to study the genetic regulatory mechanisms during growth and development.

Stress Biology Research: It can be used to analyze changes in gene expression under adverse conditions such as drought, salinità, and high temperature.

• Analisi dell'espressione genica

Real-time Quantitative PCR (qRT-PCR): The extracted RNA can be used for quantitative analysis of specific gene expression levels.

RNA Sequencing (RNA-seq): High-quality RNA is essential for transcriptome sequencing and can be used for comprehensive analysis of plant transcriptomes.

Microarray Analysis: The extracted RNA can be used for microarray analysis to study gene expression profiles.

• Esperimenti di biologia molecolare

Northern Blot and Dot Blot: These techniques are used to detect specific RNA bands or dot arrays.

PolyA Selection: It is used for enriching and studying mRNA.

In Vitro Translation: The extracted RNA can be used for in vitro translation experiments to study protein synthesis.

RNase Protection Assay: It is used to analyze RNA stability.

Molecular Cloning: The extracted RNA can be used for constructing cDNA libraries.

• High-Throughput Automated Extraction

Compatibility with Automated Extraction Devices: The magnetic bead-based extraction reagents are suitable for use with automated nucleic acid extraction instruments, allowing for rapid and efficient processing of large sample volumes.

High-Throughput Experiments: It is suitable for large-scale RNA extraction from plant samples, improving experimental efficiency.

• Altre applicazioni

Non-coding RNA Research: The extracted RNA can be used to study the functions of non-coding RNAs such as miRNA and lncRNA.

Protein Synthesis and Modification Studies: By analyzing mRNA modifications (such as m6A methylation), the impact on translation efficiency can be studied.

Environmental Microbiology: It can be used to study the RNA of microbial communities in the rhizosphere of plants.

Introduzione del fornitore

Shanghai Lingjun Biotecnologia Co., Ltd.è stato fondato nel 2016 ed è un produttore professionale di materiali biomagnetici e reagenti per l'estrazione degli acidi nucleici.

Abbiamo una ricca esperienza nell'estrazione e purificazione degli acidi nucleici, purificazione delle proteine, separazione cellulare, chemiluminescenza e altri campi tecnici.

I nostri prodotti sono ampiamente utilizzati in molti campi, come i test medici, test genetici, ricerca universitaria, allevamento genetico, e così via. Non solo forniamo prodotti, ma possiamo anche intraprendere OEM, ODM, e altre esigenze. Se hai esigenze correlate, non esitate a contattarci all'indirizzo sales01@lingjunbio.com.