FFPE 조직 DNA 추출 키트용 Lnjnbio 마그네틱 비드 221006

（Magnetic Beads Method）

【제품소개】

The paraffin-embedded tissue DNA extraction reagent uses an environmentally friendly deparaffinization method to remove paraffin, and special lysis conditions are applied to release the DNA in the tissue section, overcoming the inhibition effect caused by formalin cross-linking. The reagent uses DNA-binding magnetic beads and a unique buffer system to purify high-quality DNA while maximizing the removal of proteins, inorganic salts and other organic impurities. The obtained DNA has good integrity, 고순도, 안정적이고 믿을 수 있는 품질, and is suitable for downstream experiments such as PCR, qPCR, SNP gene analysis, 그리고 시퀀싱.

【제품의 장점】

1. Safe and non-toxic: no need for xylene dewaxing, no need for toxic organic reagents such as phenol/chloroform

2. Excellent applicability: suitable for paraffin sections of various tissues

3. 고순도: effectively remove PCR inhibitors such as proteins and inorganic salts, which is convenient for downstream experiments.

4. This kit can be used for manual extraction, and can also be integrated with a variety of high-throughput extractors.

【상품 이미지】

 

【실험사례】

사례1：

The DNA of 10 cases of paraffin-embedded tissues (5 μm, 6 sections) of human gastric mucosa was extracted by using our company’s paraffin-embedded tissue DNA extraction kit, and then qPCR was performed with human GAPDH housekeeper gene as the target gene, and whether the extracted product affected qPCR amplification.

결과 분석: The results showed that the amplification curve was S-shaped, and there was no obvious inhibition. The results show that the DNA extracted by this reagent is of excellent quality and can be directly used in downstream PCR and other follow-up experiments.

Case2：

The DNA of paraffin-embedded tissue DNA extraction reagent was used to extract the paraffin-embedded tissue (20 μm, 1 sections) of pig lung, canine pancreas, and chicken lung, and the extracted products were tested by ultra-micro-ultraviolet-visible spectrophotometer, 그리고 그 결과는 다음과 같았다:

아니요.	견본	A260/A280	A260/A230	농도(ng/μL)	Yield（μg）
1	Porcine lung embedded tissue sections	1.75	2.104	297.1	29.71
2		1.76	2.115	280.1	28.01
3	Canine pancreatic embedded tissue sections	1.754	2.089	158.4	15.84
4		1.709	2.083	195.55	19.555
5	Chicken lung embedded tissue sections	1.616	1.946	114.8	11.48
6		1.606	1.826	108.55	10.855

결과: The results showed that the reagent could stably extract DNA with good purity and high yield for different types of embedded tissues, which could be directly used in downstream PCR and other follow-up experiments.

【적용분야】

The purified DNA extracted from this kit can be directly used in downstream molecular biology experiments such as PCR, qPCR, dPCR, NGS, SNP gene analysis, STR gene identification, pathological molecular research, 등., and can be applied to identification, 질병 진단, personalized medicine and other fields.

우리의 제품은 많은 분야에서 널리 사용됩니다., 의료 테스트와 같은, 유전자 검사, 대학 연구, 유전자 육종, 등. 우리는 제품을 제공할 뿐만 아니라 OEM도 수행할 수 있습니다., ODM, 그리고 다른 필요. 관련 요구사항이 있는 경우, 언제든지 sales01@lingjunbio.com으로 문의해 주세요..

공급업체 소개

상하이 링쥔 생명공학 유한회사, 주식회사에 설립되었습니다 2016 생체자성재료 및 핵산추출시약 전문제조업체입니다..

핵산 추출 및 정제 분야에서 풍부한 경험을 보유하고 있습니다., 단백질 정제, 세포 분리, 화학발광 및 기타 기술 분야.

우리의 제품은 많은 분야에서 널리 사용됩니다., 의료 테스트와 같은, 유전자 검사, 대학 연구, 유전자 육종, 등. 우리는 제품을 제공할 뿐만 아니라 OEM도 수행할 수 있습니다., ODM, 그리고 다른 필요. 관련 요구사항이 있는 경우, 언제든지 sales01@lingjunbio.com으로 문의해 주세요..