Lnjnbio Magnetic Beads for Stool Total DNA Extraction Kit 221005

(마그네틱 비드 방식)

【제품소개】

This product is suitable for the rapid and efficient extraction of total DNA from stool samples. The extraction process uses Digestive Buffer and Proteinase K to lyses the intestinal flora cells in the stool, releasing the total DNA. The magnetic beads selectively adsorb the total DNA in the lysate under the action of the binding buffer. The small amount of impurities adsorbed by the magnetic beads is removed by the Washing Buffer. The magnetic beads release the adsorbed total DNA under the action of the Elution Buffer, and the high-quality total DNA of intestinal flora can be obtained. The total DNA of intestinal flora extracted by this product does not need toxic reagents such as phenol, chloroform, 등., and the total DNA obtained can be directly used in downstream experiments.

【제품의 장점】

1. High extraction yield: 10-60 μg of total DNA can be extracted from 200 mg of sample.

2. 폭넓은 적용성: suitable for solid or liquid stool samples from different sources.

3. Easy to operate: the extraction process can be completed within 30 min, also suitable for high-throughput operation through the automated workstation.

4. High safety: free of toxic reagents such as phenol and chloroform.

【상품 이미지】

【실험사례】

사례 1:

Total DNA was extracted from six stool samples of different sources (sampling volume of 200 mg) using theStool Total DNA Extraction Kit from Shanghai Lingjun Biotechnology Co. 주식회사. on an automated nucleic acid extraction and purification instrument, LJbio32H, and about 10 μg of total DNA could be captured with high stability.

Sample number	에이260/에이280	에이260/에이230	농도. (ng/μ엘)
1	1.76	1.91	105.6
2	1.75	1.69	82.6
3	1.77	2.39	114.9
4	1.75	1.88	107.6
56	1.77	2.43	116.4
6	1.77	2.46	115.8

사례 2：

The same stool sample was extracted multiple times (10 repetitions) using the Stool Total DNA Extraction Kit from Shanghai Lingjun Biotechnology Co., 주식회사. and quantitatively amplified by qPCR to detect 16S rRNA targets.

결과 분석：

The results showed that the Ct values for multiple extracted samples were essentially the same, thus indicating good reproducibility of the extraction kit.

【에이pplication area】

Gut microbiome research: extract bacterial, fungal, and viral DNA / RNA, support 16S rRNA and metagenomic sequencing, and analyze the association between bacterial flora and disease.

Diagnosis of infectious diseases: detection of rotavirus, norovirus, Clostridium difficile, and other pathogens, to improve the efficiency of diarrhea pathogen identification.

Early tumor screening and precision medicine: extraction of fecal ctDNA, screening of genetic mutations and methylation markers of colorectal cancer, to achieve non-invasive early diagnosis.

Genetic diseases and metabolism research: analysis of host DNA and microflora genes, research of metabolic diseases mechanism and personalized intervention strategies.

Animal health and veterinary diagnosis: detect livestock and pet pathogens, optimize disease prevention and control, and diagnosis and treatment plan.

Environmental and food safety monitoring: tracking the pathogenic bacteria and drug resistance genes in water sources and food, to ensure public health safety.

공급업체 소개

상하이 링쥔 생명공학 유한회사, 주식회사에 설립되었습니다 2016 생체자성재료 및 핵산추출시약 전문제조업체입니다..

핵산 추출 및 정제 분야에서 풍부한 경험을 보유하고 있습니다., 단백질 정제, 세포 분리, 화학발광 및 기타 기술 분야.

우리의 제품은 많은 분야에서 널리 사용됩니다., 의료 테스트와 같은, 유전자 검사, 대학 연구, 유전자 육종, 등. 우리는 제품을 제공할 뿐만 아니라 OEM도 수행할 수 있습니다., ODM, 그리고 다른 필요. 관련 요구사항이 있는 경우, 언제든지 sales01@lingjunbio.com으로 문의해 주세요..