Grânulos magnéticos Lnjnbio para kit de extração total de DNA de fezes 221005

(Método de Conta Magnética)

【Introdução do produto】

This product is suitable for the rapid and efficient extraction of total DNA from stool samples. The extraction process uses Digestive Buffer and Proteinase K to lyses the intestinal flora cells in the stool, releasing the total DNA. The magnetic beads selectively adsorb the total DNA in the lysate under the action of the binding buffer. The small amount of impurities adsorbed by the magnetic beads is removed by the Washing Buffer. The magnetic beads release the adsorbed total DNA under the action of the Elution Buffer, and the high-quality total DNA of intestinal flora can be obtained. The total DNA of intestinal flora extracted by this product does not need toxic reagents such as phenol, clorofórmio, etc., and the total DNA obtained can be directly used in downstream experiments.

【Vantagens do produto】

1. High extraction yield: 10-60 μg of total DNA can be extracted from 200 mg of sample.

2. Ampla aplicabilidade: suitable for solid or liquid stool samples from different sources.

3. Fácil de operar: the extraction process can be completed within 30 min, also suitable for high-throughput operation through the automated workstation.

4. Alta segurança: free of toxic reagents such as phenol and chloroform.

【Imagem do produto】

【Casos experimentais】

Caso 1:

Total DNA was extracted from six stool samples of different sources (sampling volume of 200 mg) using theStool Total DNA Extraction Kit from Shanghai Lingjun Biotechnology Co. Ltda. on an automated nucleic acid extraction and purification instrument, LJbio32H, and about 10 μg of total DNA could be captured with high stability.

Número da amostra	UM260/UM280	UM260/UM230	Concentração. (ng/μeu)
1	1.76	1.91	105.6
2	1.75	1.69	82.6
3	1.77	2.39	114.9
4	1.75	1.88	107.6
56	1.77	2.43	116.4
6	1.77	2.46	115.8

Caso 2：

The same stool sample was extracted multiple times (10 repetitions) using the Stool Total DNA Extraction Kit from Shanghai Lingjun Biotechnology Co., Ltda. and quantitatively amplified by qPCR to detect 16S rRNA targets.

Análise de resultados：

The results showed that the Ct values for multiple extracted samples were essentially the same, thus indicating good reproducibility of the extraction kit.

【UMárea de aplicação】

Gut microbiome research: extract bacterial, fúngico, and viral DNA / ARN, support 16S rRNA and metagenomic sequencing, and analyze the association between bacterial flora and disease.

Diagnosis of infectious diseases: detection of rotavirus, norovirus, Clostridium difficile, and other pathogens, to improve the efficiency of diarrhea pathogen identification.

Early tumor screening and precision medicine: extraction of fecal ctDNA, screening of genetic mutations and methylation markers of colorectal cancer, to achieve non-invasive early diagnosis.

Genetic diseases and metabolism research: analysis of host DNA and microflora genes, research of metabolic diseases mechanism and personalized intervention strategies.

Animal health and veterinary diagnosis: detect livestock and pet pathogens, optimize disease prevention and control, and diagnosis and treatment plan.

Environmental and food safety monitoring: tracking the pathogenic bacteria and drug resistance genes in water sources and food, to ensure public health safety.

Introdução ao fornecedor

Xangai Lingjun Biotecnologia Co., Ltda.foi estabelecido em 2016 e é um fabricante profissional de materiais biomagnéticos e reagentes de extração de ácido nucleico.

Temos vasta experiência em extração e purificação de ácidos nucleicos, purificação de proteínas, separação celular, quimioluminescência e outros campos técnicos.

Nossos produtos são amplamente utilizados em muitos campos, como exames médicos, testes genéticos, pesquisa universitária, melhoramento genético, e assim por diante. Nós não apenas fornecemos produtos, mas também podemos realizar OEM, ODM, e outras necessidades. Se você tiver necessidades relacionadas, não hesite em contactar-nos em sales01@lingjunbio.com.