Lnjnbio Magnetic Beads for Blood Genomic DNA Extraction Kit (Método de Conta Magnética)231010

【Introdução do produto】

Este produto is designed for the extraction, enriquecimento, and purification of genomic nucleic acids from fresh, congelado, or blood samples containing anticoagulants. Under the influence of guanidine salts, cellular lysis occurs, releasing the nucleic acids. Through specific adsorption by magnetic beads, the nucleic acids are separated from other contaminants in the sample. They are then washed with a buffer solution to eliminate proteins and residual guanidine salts. Ultimately, purified nucleic acids are retrieved via elution and magnetic adsorption. The resulting product is suitable for applications such as PCR amplification, nucleic acid detection assays, gene mutation analysis, and sequencing experiments.

【Vantagens do produto】

1. It enables efficient lysis and binding in one step even for challenging samples like whole blood, eliminating the need for intermediate pauses to add additional reagents.

2. The extraction process does not require the addition of Proteinase K, allowing for ambient transportation and storage conditions.

3. The extracted genomic DNA exhibits high purity, yields, and intact electrophoresis bands.

4. It boasts high extraction efficiency, completing the entire process in approximately 20 minutos, ideally suited for the extraction of 200-400 μL whole blood samples.

5. It offers strong versatility, being compatible with automated nucleic acid extraction systems from various manufacturers.

【Imagem do produto】

【Casos experimentais】

Caso 1：Experimental results of extracting fresh blood samples from different healthy individuals using the same batch of reagents：

Número da amostra	Sample volume（μL）	A260/A280	A260/A230	Conc.(ng/μL)	Elution volume（μL）	Nucleic acid yield（μg）
Amostra 1	200	1.950	1.493	59.10	80	4.728
Amostra 2		1.964	1.523	60.65		4.852
Amostra 3		1.928	1.469	74.30		5.944
Amostra 4		1.967	1.481	62.75		5.020
Amostra 5		1.982	1.797	81.30		6.504
Amostra 6		2.006	1.890	78.75		6.300
Amostra 7		1.932	1.524	66.10		5.288
Amostra 8		1.945	1.540	72.90		5.832
Amostra 9		1.956	1.728	99.90		7.992
Amostra 10		1.991	1.832	88.80		7.104
Amostra 11		1.943	1.485	78.10		6.248
Amostra 12		1.975	1.473	38.15		3.052
Amostra 13		1.917	1.459	105.80		8.464
Amostra 14		1.988	1.657	58.05		4.644
平均		1.963	1.578	70.37		5.630
Remarks：Experimental date 7/14/2023

Genomic DNA was extracted from fresh blood samples of healthy individuals using a Blood Genomic DNA Extraction Reagent kit on the automated nucleic acid extraction and purification instrument, LJbio32H. The electrophoresis results are shown in the following figure.

Sample volume：200 μL, Elution volume ：80 μL, electrophoresis loading volume:6 μL，Agarose gel concentration is 1%,120 V,time:30 mins.

Análise de resultados：

From the extracted product detection data and agarose electrophoresis results, it can be seen that：

1. The extraction time is about 20 minutos, and all 14 samples can stably extract human whole blood genomic DNA;

2. The purity of the extracted products is good: o 260/280 ratios for all extraction products are above 1.9, and the 260/230 ratios are above 1.4.

3. High yield of extraction products: Yields from all 200 μL samples are consistently above 3 μg, sufficient to meet the requirements of downstream applications.

4. The electrophoretic bands are complete and without degradable and diffuse.

Comparison Content	Shanghai Lingjun	other manufacturer
Sample volume	200-400 μL	100-250 μL
PK	No need	Need
Lysis time	8 min	15 min+10 min
Lysis temperature	65℃ or room temperature	75℃
Lysis and binding	Only one step	Comprises two steps
Washing process	Three times, 3 min each time	Four times, 3 min each time
Elution time	65℃，5 min	75℃，10 min
Drying time	1 min	5 min
Extraction purity	OD260/280 1.7-2.0	OD260/280 1.7-1.9
	OD260/230＞1.4	/
Extraction yield	200 μL sample can extract genomic DNA＞3 μg	250 μL sample can extract genomic DNA＞2 μg
Extraction time	About 20 min	More than one hour

 Caso 2：Comparison with reagent kits from other manufacturer：

Caso 3: The results of extracting 32 samples of pig whole blood using a fully automatic nucleic acid extraction and purification instrument are as follows：

Número da amostra	Sample volume（µL）	A260/A280	A260/A230	Conc.（ng/µL）	Extraction yield（µg）
1	200	1.915	1.959	94.25	9.425
2		1.906	1.978	94.3	9.43
3		1.919	2.013	99.7	9.97
4		1.919	1.983	89.4	8.94
5		1.915	2.04	85.1	8.51
6		1.92	1.952	87.55	8.755
7		1.918	1.946	94.9	9.49
8		1.906	1.9	94.6	9.46
9		1.923	2.014	87.3	8.73
10		1.915	1.924	82.5	8.25
11		1.922	1.997	85	8.5
12		1.919	2.029	90.5	9.05
13		1.941	2.005	87.6	8.76
14		1.926	1.963	92.95	9.295
15		1.934	1.98	95.15	9.515
16		1.928	1.97	102	10.2
17		1.908	1.914	91.2	9.12
18		1.909	1.907	95.6	9.56
19		1.933	1.989	100.8	10.08
20		1.931	1.902	99.15	9.915
21		1.922	1.945	97.6	9.76
22		1.915	1.984	99.7	9.97
23		1.919	1.921	83.95	8.395
24		1.937	2.005	106.05	10.605
25		1.933	1.961	101.4	10.14
26		1.927	1.958	98.85	9.885
27		1.925	1.906	89.7	8.97
28		1.932	1.892	86.55	8.655
29		1.931	1.963	94.35	9.435
30		1.94	1.961	99.55	9.955
31		1.944	2	96.6	9.66
32		1.933	1.987	88.8	8.88
Average value		1.924	1.964	93.520	9.352
Standard deviation		0.010	0.039	5.963	0.596
Coefficient of variation		0.5%	2%	6.4%	6.4%

Análise de resultados：The test results of the micro nucleic acid analyzer indicate that：

1. The coefficient of variation of A260/A280 and A260/A230 below 2%.The test results demonstrate high stability of the reagent in extracting nucleic acids.。
2. O 260/280 ratios for all extraction products between 1.906 e 1.944，o 260/280 ratios for all extraction products between 1.892 and 2.04.The intact and nearly coinciding peak profiles attest to the high purity of the extracted product.。
3. The extraction amount of nucleic acid from 200 µL pig whole blood can reach over 8.25 µ g.

Os resultados da eletroforese em gel de agarose mostraram que:

Seven samples were randomly selected, all exhibiting bright bands with good integrity.

Resumindo, 32 samples of whole blood were extracted using a fully automatic nucleic acid extraction and purification instrument. The extracted products had high purity, alto rendimento, complete electrophoresis bands, and uniform and stable combination of reagents and instruments.

【Área de aplicação】

The nucleic acids purified by this extraction reagent are suitable for various routine experiments including enzymatic digestion, PCR, construção de biblioteca, Southern blotting, as well as applications in microarray hybridization and high-throughput sequencing.

Introdução ao fornecedor

Xangai Lingjun Biotecnologia Co., Ltda.foi estabelecido em 2016 e é um fabricante profissional de materiais biomagnéticos e reagentes de extração de ácido nucleico.

Temos vasta experiência em extração e purificação de ácidos nucleicos, purificação de proteínas, separação celular, quimioluminescência e outros campos técnicos.

Nossos produtos são amplamente utilizados em muitos campos, como exames médicos, testes genéticos, pesquisa universitária, melhoramento genético, e assim por diante. Nós não apenas fornecemos produtos, mas também podemos realizar OEM, ODM, e outras necessidades. Se você tiver necessidades relacionadas, não hesite em contactar-nos em sales01@lingjunbio.com.