Lnjnbio Coirníní Maighnéadacha le haghaidh Trealamh Eastóscadh DNA Genomic Fola (Modh Coirnín Maighnéadach)231010

【Réamhrá Táirge】

An táirge seo is designed for the extraction, saibhriú, agus íonú aigéid núicléasaigh ghéanómacha ó úr, reoite, or blood samples containing anticoagulants. Under the influence of guanidine salts, cellular lysis occurs, releasing the nucleic acids. Through specific adsorption by magnetic beads, the nucleic acids are separated from other contaminants in the sample. They are then washed with a buffer solution to eliminate proteins and residual guanidine salts. I ndeireadh na dála, purified nucleic acids are retrieved via elution and magnetic adsorption. The resulting product is suitable for applications such as PCR amplification, nucleic acid detection assays, gene mutation analysis, and sequencing experiments.

【Buntáistí táirge】

1. It enables efficient lysis and binding in one step even for challenging samples like whole blood, eliminating the need for intermediate pauses to add additional reagents.

2. Ní gá Proteinase K a chur leis an bpróiseas eastósctha, allowing for ambient transportation and storage conditions.

3. Léiríonn an DNA géanóm a bhaintear íonacht ard, yields, agus bannaí leictreafóiréis slán.

4. It boasts high extraction efficiency, completing the entire process in approximately 20 nóiméad, ideally suited for the extraction of 200-400 μL whole blood samples.

5. It offers strong versatility, being compatible with automated nucleic acid extraction systems from various manufacturers.

【Íomhá táirge】

【Cásanna turgnamhacha】

Cás 1：Experimental results of extracting fresh blood samples from different healthy individuals using the same batch of reagents：

Uimhir shamplach	Toirt sampla （μL）	A260/A280	A260/A230	Conc.(ng/μL)	Toirt elution (μL）	Táirgeacht aigéid núicléasaigh （μg）
Sampla 1	200	1.950	1.493	59.10	80	4.728
Sampla 2		1.964	1.523	60.65		4.852
Sampla 3		1.928	1.469	74.30		5.944
Sampla 4		1.967	1.481	62.75		5.020
Sampla 5		1.982	1.797	81.30		6.504
Sampla 6		2.006	1.890	78.75		6.300
Sampla 7		1.932	1.524	66.10		5.288
Sampla 8		1.945	1.540	72.90		5.832
Sampla 9		1.956	1.728	99.90		7.992
Sampla 10		1.991	1.832	88.80		7.104
Sampla 11		1.943	1.485	78.10		6.248
Sampla 12		1.975	1.473	38.15		3.052
Sampla 13		1.917	1.459	105.80		8.464
Sampla 14		1.988	1.657	58.05		4.644
平均		1.963	1.578	70.37		5.630
Remarks：Experimental date 7/14/2023

Genomic DNA was extracted from fresh blood samples of healthy individuals using a Blood Genomic DNA Extraction Reagent kit on the automated nucleic acid extraction and purification instrument, LJbio32H. The electrophoresis results are shown in the following figure.

Toirt samplach：200 μL, Elution volume ：80 μL, electrophoresis loading volume:6 μL，Agarose gel concentration is 1%,120 V,am:30 mins.

Anailís ar thorthaí：

From the extracted product detection data and agarose electrophoresis results, is féidir é a fheiceáil go：

1. The extraction time is about 20 nóiméad, and all 14 samples can stably extract human whole blood genomic DNA;

2. The purity of the extracted products is good: an 260/280 ratios for all extraction products are above 1.9, agus an 260/230 ratios are above 1.4.

3. High yield of extraction products: Yields from all 200 μL samples are consistently above 3 μg, sufficient to meet the requirements of downstream applications.

4. The electrophoretic bands are complete and without degradable and diffuse.

Ábhar Comparáide	Shanghai Lingjun	monaróir eile
Toirt samplach	200-400 μL	100-250 μL
PK	Ní gá	Gá
Am Lysis	8 nóim	15 nóim+10 nóim
Lysis teocht	65℃ nó teocht an tseomra	75℃
Lysis agus ceangailteach	Níl ach céim amháin	Tá dhá chéim i gceist
Próiseas níocháin	Trí huaire, 3 min gach uair	Ceithre huaire, 3 min gach uair
Am elution	65℃，5 nóim	75℃，10 nóim
Am a thriomú	1 nóim	5 nóim
íonacht eastóscadh	OD260/280 1.7-2.0	OD260/280 1.7-1.9
	OD260/230＞ 1.4	/
Toradh eastósctha	200 Is féidir le sampla μL DNA géanóm a bhaint as ＞3 μg	250 Is féidir le sampla μL DNA géanóm a bhaint as ＞2 μg
Am eastósctha	Maidir 20 nóim	Níos mó ná uair an chloig

 Cás 2：Comparáid le feisteáin imoibrí ó mhonaróir eile：

Cás 3: The results of extracting 32 samples of pig whole blood using a fully automatic nucleic acid extraction and purification instrument are as follows：

Uimhir shamplach	Sample volume（µL）	A260/A280	A260/A230	Conc.（ng/µL）	Extraction yield（µg）
1	200	1.915	1.959	94.25	9.425
2		1.906	1.978	94.3	9.43
3		1.919	2.013	99.7	9.97
4		1.919	1.983	89.4	8.94
5		1.915	2.04	85.1	8.51
6		1.92	1.952	87.55	8.755
7		1.918	1.946	94.9	9.49
8		1.906	1.9	94.6	9.46
9		1.923	2.014	87.3	8.73
10		1.915	1.924	82.5	8.25
11		1.922	1.997	85	8.5
12		1.919	2.029	90.5	9.05
13		1.941	2.005	87.6	8.76
14		1.926	1.963	92.95	9.295
15		1.934	1.98	95.15	9.515
16		1.928	1.97	102	10.2
17		1.908	1.914	91.2	9.12
18		1.909	1.907	95.6	9.56
19		1.933	1.989	100.8	10.08
20		1.931	1.902	99.15	9.915
21		1.922	1.945	97.6	9.76
22		1.915	1.984	99.7	9.97
23		1.919	1.921	83.95	8.395
24		1.937	2.005	106.05	10.605
25		1.933	1.961	101.4	10.14
26		1.927	1.958	98.85	9.885
27		1.925	1.906	89.7	8.97
28		1.932	1.892	86.55	8.655
29		1.931	1.963	94.35	9.435
30		1.94	1.961	99.55	9.955
31		1.944	2	96.6	9.66
32		1.933	1.987	88.8	8.88
Average value		1.924	1.964	93.520	9.352
Standard deviation		0.010	0.039	5.963	0.596
Coefficient of variation		0.5%	2%	6.4%	6.4%

Anailís ar thorthaí：The test results of the micro nucleic acid analyzer indicate that：

1. The coefficient of variation of A260/A280 and A260/A230 below 2%.The test results demonstrate high stability of the reagent in extracting nucleic acids.。
2. Tá an 260/280 ratios for all extraction products between 1.906 agus 1.944，an 260/280 ratios for all extraction products between 1.892 and 2.04.The intact and nearly coinciding peak profiles attest to the high purity of the extracted product.。
3. The extraction amount of nucleic acid from 200 µL pig whole blood can reach over 8.25 g.

Léirigh torthaí leictreafóiréis glóthach agarose go:

Seven samples were randomly selected, all exhibiting bright bands with good integrity.

Go hachomair, 32 samples of whole blood were extracted using a fully automatic nucleic acid extraction and purification instrument. The extracted products had high purity, toradh ard, complete electrophoresis bands, and uniform and stable combination of reagents and instruments.

【Limistéar iarratais】

The nucleic acids purified by this extraction reagent are suitable for various routine experiments including enzymatic digestion, PCR, tógáil leabharlainne, Blotting Theas, as well as applications in microarray hybridization and high-throughput sequencing.

Réamhrá Soláthraí

Shanghai Lingjun Biteicneolaíocht Co., Teo.bunaíodh i 2016 agus is monaróir gairmiúil ábhair bhithmhaighnéadacha agus imoibrithe eastóscadh aigéid núicléacha.

Tá taithí shaibhir againn in eastóscadh agus íonú aigéad núicléasach, íonú próitéine, scaradh cille, chemiluminescence agus réimsí teicniúla eile.

Úsáidtear ár gcuid táirgí go forleathan i go leor réimsí, mar thástáil leighis, tástáil ghéiniteach, taighde ollscoile, pórú géiniteach, agus mar sin de. Ní hamháin go gcuirimid táirgí ar fáil ach is féidir linn OEM a dhéanamh freisin, ODM, agus riachtanais eile. Má tá riachtanais ghaolmhara agat, bíodh leisce ort teagmháil a dhéanamh linn ag sales01@lingjunbio.com.