Lnjnbio Kit de extracción de ADN genómico de perlas magnéticas para sangre (Método de cuentas magnéticas)231010

【Introducción del producto】

This product is designed for the extraction, enrichment, and purification of genomic nucleic acids from fresh, frozen, or blood samples containing anticoagulants. Under the influence of guanidine salts, cellular lysis occurs, releasing the nucleic acids. Through specific adsorption by magnetic beads, the nucleic acids are separated from other contaminants in the sample. They are then washed with a buffer solution to eliminate proteins and residual guanidine salts. Al final, purified nucleic acids are retrieved via elution and magnetic adsorption. The resulting product is suitable for applications such as PCR amplification, nucleic acid detection assays, gene mutation analysis, and sequencing experiments.

【Ventajas del producto】

1. It enables efficient lysis and binding in one step even for challenging samples like whole blood, eliminating the need for intermediate pauses to add additional reagents.

2. The extraction process does not require the addition of Proteinase K, allowing for ambient transportation and storage conditions.

3. The extracted genomic DNA exhibits high purity, yields, and intact electrophoresis bands.

4. It boasts high extraction efficiency, completing the entire process in approximately 20 minutos, ideally suited for the extraction of 200-400 μL whole blood samples.

5. It offers strong versatility, being compatible with automated nucleic acid extraction systems from various manufacturers.

【Imagen del producto】

【Casos experimentales】

Caso 1：Experimental results of extracting fresh blood samples from different healthy individuals using the same batch of reagents：

Sample number	Sample volume（µL）	A260/A280	A260/A230	Conc.(ng/μL)	Elution volume（µL）	Nucleic acid yield（µg）
Muestra 1	200	1.950	1.493	59.10	80	4.728
Muestra 2		1.964	1.523	60.65		4.852
Muestra 3		1.928	1.469	74.30		5.944
Muestra 4		1.967	1.481	62.75		5.020
Muestra 5		1.982	1.797	81.30		6.504
Muestra 6		2.006	1.890	78.75		6.300
Muestra 7		1.932	1.524	66.10		5.288
Muestra 8		1.945	1.540	72.90		5.832
Muestra 9		1.956	1.728	99.90		7.992
Muestra 10		1.991	1.832	88.80		7.104
Muestra 11		1.943	1.485	78.10		6.248
Muestra 12		1.975	1.473	38.15		3.052
Muestra 13		1.917	1.459	105.80		8.464
Muestra 14		1.988	1.657	58.05		4.644
平均		1.963	1.578	70.37		5.630
Remarks：Experimental date 7/14/2023

Genomic DNA was extracted from fresh blood samples of healthy individuals using a Blood Genomic DNA Extraction Reagent kit on the automated nucleic acid extraction and purification instrument, LJbio32H. The electrophoresis results are shown in the following figure.

Sample volume：200 µL, Elution volume ：80 µL, electrophoresis loading volume:6 µL，Agarose gel concentration is 1%,120 V,time:30 mins.

Análisis de resultados：

From the extracted product detection data and agarose electrophoresis results, it can be seen that：

1. The extraction time is about 20 minutos, and all 14 samples can stably extract human whole blood genomic DNA;

2. The purity of the extracted products is good: el 260/280 ratios for all extraction products are above 1.9, and the 260/230 ratios are above 1.4.

3. High yield of extraction products: Yields from all 200 μL samples are consistently above 3 µg, sufficient to meet the requirements of downstream applications.

4. The electrophoretic bands are complete and without degradable and diffuse.

Comparison Content	Shanghái Lingjun	other manufacturer
Sample volume	200-400 µL	100-250 µL
PK	No need	Need
Lysis time	8 mín.	15 min+10 min
Lysis temperature	65℃ or room temperature	75℃
Lysis and binding	Only one step	Comprises two steps
Washing process	Three times, 3 min each time	Four times, 3 min each time
Elution time	65℃，5 mín.	75℃，10 mín.
Drying time	1 mín.	5 mín.
Extraction purity	OD260/280 1.7-2.0	OD260/280 1.7-1.9
	OD260/230＞1.4	/
Extraction yield	200 μL sample can extract genomic DNA＞3 μg	250 μL sample can extract genomic DNA＞2 μg
Extraction time	About 20 mín.	More than one hour

 Caso 2：Comparison with reagent kits from other manufacturer：

Caso 3: The results of extracting 32 samples of pig whole blood using a fully automatic nucleic acid extraction and purification instrument are as follows：

Sample number	Sample volume（µL）	A260/A280	A260/A230	Conc.（ng/µL）	Extraction yield（µg）
1	200	1.915	1.959	94.25	9.425
2		1.906	1.978	94.3	9.43
3		1.919	2.013	99.7	9.97
4		1.919	1.983	89.4	8.94
5		1.915	2.04	85.1	8.51
6		1.92	1.952	87.55	8.755
7		1.918	1.946	94.9	9.49
8		1.906	1.9	94.6	9.46
9		1.923	2.014	87.3	8.73
10		1.915	1.924	82.5	8.25
11		1.922	1.997	85	8.5
12		1.919	2.029	90.5	9.05
13		1.941	2.005	87.6	8.76
14		1.926	1.963	92.95	9.295
15		1.934	1.98	95.15	9.515
16		1.928	1.97	102	10.2
17		1.908	1.914	91.2	9.12
18		1.909	1.907	95.6	9.56
19		1.933	1.989	100.8	10.08
20		1.931	1.902	99.15	9.915
21		1.922	1.945	97.6	9.76
22		1.915	1.984	99.7	9.97
23		1.919	1.921	83.95	8.395
24		1.937	2.005	106.05	10.605
25		1.933	1.961	101.4	10.14
26		1.927	1.958	98.85	9.885
27		1.925	1.906	89.7	8.97
28		1.932	1.892	86.55	8.655
29		1.931	1.963	94.35	9.435
30		1.94	1.961	99.55	9.955
31		1.944	2	96.6	9.66
32		1.933	1.987	88.8	8.88
Average value		1.924	1.964	93.520	9.352
Standard deviation		0.010	0.039	5.963	0.596
Coefficient of variation		0.5%	2%	6.4%	6.4%

Análisis de resultados：The test results of the micro nucleic acid analyzer indicate that：

1. The coefficient of variation of A260/A280 and A260/A230 below 2%.The test results demonstrate high stability of the reagent in extracting nucleic acids.。
2. El 260/280 ratios for all extraction products between 1.906 y 1.944，el 260/280 ratios for all extraction products between 1.892 and 2.04.The intact and nearly coinciding peak profiles attest to the high purity of the extracted product.。
3. The extraction amount of nucleic acid from 200 µL pig whole blood can reach over 8.25 µ g.

The results of agarose gel electrophoresis showed that:

Seven samples were randomly selected, all exhibiting bright bands with good integrity.

En resumen, 32 samples of whole blood were extracted using a fully automatic nucleic acid extraction and purification instrument. The extracted products had high purity, alto rendimiento, complete electrophoresis bands, and uniform and stable combination of reagents and instruments.

【Área de aplicación】

The nucleic acids purified by this extraction reagent are suitable for various routine experiments including enzymatic digestion, PCR, construcción de biblioteca, Southern blotting, as well as applications in microarray hybridization and high-throughput sequencing.

Introducción del proveedor

Shanghai Lingjun Biotecnología Co., Limitado.se estableció en 2016 y es un fabricante profesional de materiales biomagnéticos y reactivos de extracción de ácidos nucleicos..

Tenemos una rica experiencia en extracción y purificación de ácidos nucleicos., purificación de proteínas, separación celular, quimioluminiscencia y otros campos técnicos.

Nuestros productos son ampliamente utilizados en muchos campos., como pruebas médicas, pruebas genéticas, investigación universitaria, mejoramiento genético, etcétera. No solo proporcionamos productos sino que también podemos realizar OEM, ODM, y otras necesidades. Si tienes necesidades relacionadas, no dude en contactarnos en sales01@lingjunbio.com.