Lnjnbio Perlas magnéticas para kit de extracción de ADN genómico de tejidos animales 231014

(Método de cuentas magnéticas, No Centrifugation)

【Introducción del producto】

Various animal tissues and cultured cells, such as animal muscle, liver, spleen, etc., release nucleic acid under the action of lysate, and the nucleic acid is separated from other impurities in the sample by the specific adsorption of magnetic beads, and then rinsed by the washing solution to remove proteins, polisacáridos, inorganic salt ions, etc., and finally, after the elution, we get the purified nucleic acid, and the nucleic acid product obtained can be used for PCR, nucleic acid detection and analysis, hibridación molecular, gene mutation detection and sequencing and other experiments.

【Ventajas del producto】

1, Extraction by magnetic bead method without centrifugation, simple operation, and can realize automatic operation.

2, Alta pureza, bandas electroforéticas completas y de alto rendimiento.

3, Gran versatilidad, suitable for extracting genomic DNA from various tissue samples of different animal species.

4, Safe to use, no need to grind with liquid nitrogen, and the product does not contain harmful reagents such as phenol and chloroform.

【Imagen del producto】

【Experimental case】

Different tissue samples from different animals were extracted for DNA/RNA

 Caso1: 

No.	Sample intake	A260/A280	A260/A230	Conc.(de/ul)	Producir（μg）
1	30mg Pork	1.886	2.037	113.1	11.31
2		1.897	2.058	113.4	11.34
promedio		1.892	2.048	113.25	11.325
3	20mg Shrimp	1.889	2.06	139.5	13.95
4		1.894	2.064	141.05	14.105
promedio		1.892	2.062	140.275	14.028
5	30mg Beef	1.837	2.001	135.2	13.52
6		1.842	2.037	135	13.5
promedio		1.834	2.019	135.1	13.51
7	30mg Mutton	1.851	2.043	175.75	17.575
8		1.84	2.073	180.25	18.025
promedio		1.846	2.058	178	17.8
9	20mg Chicken	1.889	2.06	139.5	13.95
10		1.894	2.064	141.05	14.105
promedio		1.892	2.062	140.275	14.028
11	50mg Fish	1.873	2.097	237	23.7
12		1.875	2.083	233.2	23.32
promedio		1.874	2.09	235.1	23.51
13	3mg Fish heart	1.827	2.236	140.4	14.04
14		1.818	2.201	145.7	14.57
promedio		1.823	2.219	143.05	14.305
15	3mg Fish liver	1.791	2.295	293.3	29.33
16		1.781	2.308	326.7	32.67
promedio		1.786	2.302	310	31

Peak pattern：

Pork：

Shrimp：

Beef：

Mutton：

Chicken：

Fish：

Fish heart：

Fish liver：

El electroferograma se muestra a continuación.：

METRO:Marker（2K，1K，750pb，500pb，250pb，100pb）;

1-2：30mg Pork 3-4：20mg Shrimp 5-6：30mg Beef 7-8：30mg Mutton

9-10：20mg Chicken 11-12：50mg Fish 13-14：3mg Fish heart 15-16：3mg Fish liver

Analysis of result：

The test results of the ultra-micro nucleic acid analyzer showed that:

①The value of A260/A280 is 1.78-1.9, and the value of A260/A230 is above 2.0, and the peak shape is complete and basically coincides, which proves that the purity of the extracted product is high.

②The yield of nucleic acid extraction from different animal tissue samples was more than 11 µg.

The results of agarose gel electrophoresis showed that:

①Tissue samples from different animals all had bright bands and good integrity.

En resumen: the automatic nucleic acid extraction and purification instrument was used to extract different animal samples, and the extracted products had high purity, alto rendimiento, complete electrophoresis bands, and the reagents and instruments were uniform and stable.

【Área de aplicación】

Genotyping of fresh or frozen animal tissues and cultured cells, high-throughput PCR screening of large-scale biological samples, transgene screening, knockout analysis and sequencing, enzyme digestion, PCR, Southern-Blot and library construction and other experiments.

Introducción del proveedor

Shanghai Lingjun Biotecnología Co., Limitado.se estableció en 2016 y es un fabricante profesional de materiales biomagnéticos y reactivos de extracción de ácidos nucleicos..

Tenemos una rica experiencia en extracción y purificación de ácidos nucleicos., purificación de proteínas, separación celular, quimioluminiscencia y otros campos técnicos.

Nuestros productos son ampliamente utilizados en muchos campos., como pruebas médicas, pruebas genéticas, investigación universitaria, mejoramiento genético, etcétera. No solo proporcionamos productos sino que también podemos realizar OEM, ODM, y otras necesidades. Si tienes necesidades relacionadas, no dude en contactarnos en sales01@lingjunbio.com.