Lnjnbio Magnetic Beads for Animal Tissue Genomic DNA Extraction Kit 231014

(طريقة الخرزة المغناطيسية, No Centrifugation)

【Product Introduction】

Various animal tissues and cultured cells, such as animal muscle, liver, spleen, إلخ., release nucleic acid under the action of lysate, and the nucleic acid is separated from other impurities in the sample by the specific adsorption of magnetic beads, and then rinsed by the washing solution to remove proteins, polysaccharides, inorganic salt ions, إلخ., and finally, after the elution, we get the purified nucleic acid, and the nucleic acid product obtained can be used for PCR, nucleic acid detection and analysis, molecular hybridization, gene mutation detection and sequencing and other experiments.

【Product advantages】

1, Extraction by magnetic bead method without centrifugation, simple operation, and can realize automatic operation.

2, High purity, high yield and complete electrophoretic bands.

3, Strong versatility, suitable for extracting genomic DNA from various tissue samples of different animal species.

4, Safe to use, no need to grind with liquid nitrogen, and the product does not contain harmful reagents such as phenol and chloroform.

【Product image】

【Experimental case】

Different tissue samples from different animals were extracted for DNA/RNA

 Case1: 

No.	Sample intake	A260/A280	A260/A230	Conc.(ng/ul)	Yield（μg）
1	30mg Pork	1.886	2.037	113.1	11.31
2		1.897	2.058	113.4	11.34
Avg		1.892	2.048	113.25	11.325
3	20mg Shrimp	1.889	2.06	139.5	13.95
4		1.894	2.064	141.05	14.105
Avg		1.892	2.062	140.275	14.028
5	30mg Beef	1.837	2.001	135.2	13.52
6		1.842	2.037	135	13.5
Avg		1.834	2.019	135.1	13.51
7	30mg Mutton	1.851	2.043	175.75	17.575
8		1.84	2.073	180.25	18.025
Avg		1.846	2.058	178	17.8
9	20mg Chicken	1.889	2.06	139.5	13.95
10		1.894	2.064	141.05	14.105
Avg		1.892	2.062	140.275	14.028
11	50mg Fish	1.873	2.097	237	23.7
12		1.875	2.083	233.2	23.32
Avg		1.874	2.09	235.1	23.51
13	3mg Fish heart	1.827	2.236	140.4	14.04
14		1.818	2.201	145.7	14.57
Avg		1.823	2.219	143.05	14.305
15	3mg Fish liver	1.791	2.295	293.3	29.33
16		1.781	2.308	326.7	32.67
Avg		1.786	2.302	310	31

Peak pattern：

Pork：

Shrimp：

Beef：

Mutton：

Chicken：

Fish：

Fish heart：

Fish liver：

The electropherogram is shown below：

م:Marker（2K，1K，750bp，500bp，250bp，100bp）;

1-2：30mg Pork 3-4：20mg Shrimp 5-6：30mg Beef 7-8：30mg Mutton

9-10：20mg Chicken 11-12：50mg Fish 13-14：3mg Fish heart 15-16：3mg Fish liver

Analysis of result：

The test results of the ultra-micro nucleic acid analyzer showed that:

①The value of A260/A280 is 1.78-1.9, and the value of A260/A230 is above 2.0, and the peak shape is complete and basically coincides, which proves that the purity of the extracted product is high.

②The yield of nucleic acid extraction from different animal tissue samples was more than 11 μg.

The results of agarose gel electrophoresis showed that:

①Tissue samples from different animals all had bright bands and good integrity.

في ملخص: the automatic nucleic acid extraction and purification instrument was used to extract different animal samples, and the extracted products had high purity, high yield, complete electrophoresis bands, and the reagents and instruments were uniform and stable.

【Application area】

Genotyping of fresh or frozen animal tissues and cultured cells, high-throughput PCR screening of large-scale biological samples, transgene screening, knockout analysis and sequencing, enzyme digestion, تفاعل البوليميراز المتسلسل, Southern-Blot and library construction and other experiments.

مقدمة المورد

شركة شنغهاي لينغجون للتكنولوجيا الحيوية, المحدودة.تأسست في 2016 and is a professional manufacturer of biomagnetic materials and nucleic acid extraction reagents.

لدينا خبرة غنية في استخراج وتنقية الحمض النووي, تنقية البروتين, فصل الخلايا, التألق الكيميائي والمجالات التقنية الأخرى.

منتجاتنا تستخدم على نطاق واسع في العديد من المجالات, مثل الفحوصات الطبية, الاختبارات الجينية, البحوث الجامعية, التكاثر الوراثي, وهكذا. نحن لا نقدم المنتجات فحسب، بل يمكننا أيضًا إجراء تصنيع المعدات الأصلية, أوديإم, وغيرها من الاحتياجات. إذا كان لديك الاحتياجات ذات الصلة, لا تتردد في الاتصال بنا على sales01@lingjunbio.com.