Lnjnbio 動物組織ゲノム DNA 抽出キット用磁気ビーズ 231014

(磁気ビーズ法, No Centrifugation)

【商品紹介】

Various animal tissues and cultured cells, such as animal muscle, liver, spleen, 等, release nucleic acid under the action of lysate, and the nucleic acid is separated from other impurities in the sample by the specific adsorption of magnetic beads, and then rinsed by the washing solution to remove proteins, 多糖類, inorganic salt ions, 等, and finally, after the elution, we get the purified nucleic acid, and the nucleic acid product obtained can be used for PCR, nucleic acid detection and analysis, 分子ハイブリダイゼーション, gene mutation detection and sequencing and other experiments.

【製品の利点】

1, Extraction by magnetic bead method without centrifugation, simple operation, and can realize automatic operation.

2, 高純度, 高収率で完全な電気泳動バンド.

3, 強力な汎用性, suitable for extracting genomic DNA from various tissue samples of different animal species.

4, Safe to use, no need to grind with liquid nitrogen, and the product does not contain harmful reagents such as phenol and chloroform.

【商品画像】

【Experimental case】

Different tissue samples from different animals were extracted for DNA/RNA

 場合1: 

No.	Sample intake	A260/A280	A260/A230	濃度(の/ul)	収率（μg）
1	30mg Pork	1.886	2.037	113.1	11.31
2		1.897	2.058	113.4	11.34
平均		1.892	2.048	113.25	11.325
3	20mg Shrimp	1.889	2.06	139.5	13.95
4		1.894	2.064	141.05	14.105
平均		1.892	2.062	140.275	14.028
5	30mg Beef	1.837	2.001	135.2	13.52
6		1.842	2.037	135	13.5
平均		1.834	2.019	135.1	13.51
7	30mg Mutton	1.851	2.043	175.75	17.575
8		1.84	2.073	180.25	18.025
平均		1.846	2.058	178	17.8
9	20mg Chicken	1.889	2.06	139.5	13.95
10		1.894	2.064	141.05	14.105
平均		1.892	2.062	140.275	14.028
11	50mg Fish	1.873	2.097	237	23.7
12		1.875	2.083	233.2	23.32
平均		1.874	2.09	235.1	23.51
13	3mg Fish heart	1.827	2.236	140.4	14.04
14		1.818	2.201	145.7	14.57
平均		1.823	2.219	143.05	14.305
15	3mg Fish liver	1.791	2.295	293.3	29.33
16		1.781	2.308	326.7	32.67
平均		1.786	2.302	310	31

Peak pattern：

Pork：

Shrimp：

Beef：

Mutton：

Chicken：

Fish：

Fish heart：

Fish liver：

エレクトロフェログラムを以下に示します：

M:Marker（2K，1K，750血圧，500血圧，250血圧，100血圧）;

1-2：30mg Pork 3-4：20mg Shrimp 5-6：30mg Beef 7-8：30mg Mutton

9-10：20mg Chicken 11-12：50mg Fish 13-14：3mg Fish heart 15-16：3mg Fish liver

Analysis of result：

The test results of the ultra-micro nucleic acid analyzer showed that:

①The value of A260/A280 is 1.78-1.9, and the value of A260/A230 is above 2.0, and the peak shape is complete and basically coincides, which proves that the purity of the extracted product is high.

②The yield of nucleic acid extraction from different animal tissue samples was more than 11 μg.

The results of agarose gel electrophoresis showed that:

①Tissue samples from different animals all had bright bands and good integrity.

要約すれば: the automatic nucleic acid extraction and purification instrument was used to extract different animal samples, and the extracted products had high purity, 高収量, complete electrophoresis bands, and the reagents and instruments were uniform and stable.

【適用範囲】

Genotyping of fresh or frozen animal tissues and cultured cells, high-throughput PCR screening of large-scale biological samples, transgene screening, knockout analysis and sequencing, enzyme digestion, PCR, Southern-Blot and library construction and other experiments.

サプライヤー紹介

上海霊軍生物技術有限公司, 株式会社に設立されました 2016 生体磁性材料と核酸抽出試薬の専門メーカーです.

核酸抽出・精製の経験が豊富です。, タンパク質の精製, 細胞分離, 化学発光およびその他の技術分野.

当社の製品はさまざまな分野で広く使用されています, 医療検査など, 遺伝子検査, 大学の研究, 遺伝子育種, 等々. 製品のご提供だけでなく、OEMも承ります。, ODM, その他のニーズ. 関連するニーズがある場合, sales01@lingjunbio.comまでお気軽にお問い合わせください。.