Manik Magnetik Lnjnbio untuk Kit Pengekstrakan DNA Genomik Tisu Haiwan 231014

(Kaedah Manik Magnet, No Centrifugation)

【Pengenalan Produk】

Various animal tissues and cultured cells, such as animal muscle, liver, spleen, dll., release nucleic acid under the action of lysate, and the nucleic acid is separated from other impurities in the sample by the specific adsorption of magnetic beads, and then rinsed by the washing solution to remove proteins, polysaccharides, ion garam tak organik, dll., and finally, after the elution, we get the purified nucleic acid, and the nucleic acid product obtained can be used for PCR, nucleic acid detection and analysis, hibridisasi molekul, gene mutation detection and sequencing and other experiments.

【Kelebihan produk】

1, Extraction by magnetic bead method without centrifugation, simple operation, and can realize automatic operation.

2, Kesucian yang tinggi, hasil tinggi dan jalur elektroforesis lengkap.

3, Strong versatility, suitable for extracting genomic DNA from various tissue samples of different animal species.

4, Selamat digunakan, no need to grind with liquid nitrogen, and the product does not contain harmful reagents such as phenol and chloroform.

【Imej produk】

【Experimental case】

Different tissue samples from different animals were extracted for DNA/RNA

 Kes1: 

Tidak.	Sample intake	A260/A280	A260/A230	Conc.(daripada/ul)	hasil（μg）
1	30mg Pork	1.886	2.037	113.1	11.31
2		1.897	2.058	113.4	11.34
Purata		1.892	2.048	113.25	11.325
3	20mg Shrimp	1.889	2.06	139.5	13.95
4		1.894	2.064	141.05	14.105
Purata		1.892	2.062	140.275	14.028
5	30mg Beef	1.837	2.001	135.2	13.52
6		1.842	2.037	135	13.5
Purata		1.834	2.019	135.1	13.51
7	30mg Mutton	1.851	2.043	175.75	17.575
8		1.84	2.073	180.25	18.025
Purata		1.846	2.058	178	17.8
9	20mg Chicken	1.889	2.06	139.5	13.95
10		1.894	2.064	141.05	14.105
Purata		1.892	2.062	140.275	14.028
11	50mg Fish	1.873	2.097	237	23.7
12		1.875	2.083	233.2	23.32
Purata		1.874	2.09	235.1	23.51
13	3mg Fish heart	1.827	2.236	140.4	14.04
14		1.818	2.201	145.7	14.57
Purata		1.823	2.219	143.05	14.305
15	3mg Fish liver	1.791	2.295	293.3	29.33
16		1.781	2.308	326.7	32.67
Purata		1.786	2.302	310	31

Peak pattern：

Pork：

Shrimp：

Beef：

Mutton：

Chicken：

Fish：

Fish heart：

Fish liver：

Elektroferogram ditunjukkan di bawah：

M:Marker（2K，1K，750bp，500bp，250bp，100bp）;

1-2：30mg Pork 3-4：20mg Shrimp 5-6：30mg Beef 7-8：30mg Mutton

9-10：20mg Chicken 11-12：50mg Fish 13-14：3mg Fish heart 15-16：3mg Fish liver

Analysis of result：

The test results of the ultra-micro nucleic acid analyzer showed that:

①The value of A260/A280 is 1.78-1.9, and the value of A260/A230 is above 2.0, and the peak shape is complete and basically coincides, which proves that the purity of the extracted product is high.

②The yield of nucleic acid extraction from different animal tissue samples was more than 11 μg.

Keputusan elektroforesis gel agarose menunjukkan bahawa:

①Tissue samples from different animals all had bright bands and good integrity.

Secara ringkasnya: the automatic nucleic acid extraction and purification instrument was used to extract different animal samples, and the extracted products had high purity, hasil yang tinggi, jalur elektroforesis lengkap, and the reagents and instruments were uniform and stable.

【Kawasan permohonan】

Genotyping of fresh or frozen animal tissues and cultured cells, high-throughput PCR screening of large-scale biological samples, transgene screening, knockout analysis and sequencing, pencernaan enzim, PCR, Southern-Blot and library construction and other experiments.

Pengenalan Pembekal

Shanghai Lingjun Biotechnology Co., Ltd.ditubuhkan pada 2016 dan merupakan pengilang profesional bahan biomagnet dan reagen pengekstrakan asid nukleik.

Kami mempunyai pengalaman yang kaya dalam pengekstrakan dan penulenan asid nukleik, penulenan protein, pemisahan sel, chemiluminescence dan bidang teknikal lain.

Produk kami digunakan secara meluas dalam pelbagai bidang, seperti ujian perubatan, ujian genetik, penyelidikan universiti, pembiakan genetik, dan seterusnya. Kami bukan sahaja menyediakan produk tetapi juga boleh menjalankan OEM, ODM, dan keperluan lain. Jika anda mempunyai keperluan yang berkaitan, sila hubungi kami di sales01@lingjunbio.com.