Grânulos magnéticos Lnjnbio para kit de extração de DNA genômico de tecido animal 231014

(Método de Conta Magnética, No Centrifugation)

【Introdução do produto】

Various animal tissues and cultured cells, such as animal muscle, liver, spleen, etc., release nucleic acid under the action of lysate, and the nucleic acid is separated from other impurities in the sample by the specific adsorption of magnetic beads, and then rinsed by the washing solution to remove proteins, polissacarídeos, inorganic salt ions, etc., and finally, after the elution, we get the purified nucleic acid, and the nucleic acid product obtained can be used for PCR, nucleic acid detection and analysis, hibridização molecular, gene mutation detection and sequencing and other experiments.

【Vantagens do produto】

1, Extraction by magnetic bead method without centrifugation, simple operation, and can realize automatic operation.

2, Alta pureza, bandas eletroforéticas completas e de alto rendimento.

3, Forte versatilidade, suitable for extracting genomic DNA from various tissue samples of different animal species.

4, Safe to use, no need to grind with liquid nitrogen, and the product does not contain harmful reagents such as phenol and chloroform.

【Imagem do produto】

【Experimental case】

Different tissue samples from different animals were extracted for DNA/RNA

 Caso1: 

No.	Sample intake	A260/A280	A260/A230	Conc.(de/ul)	Colheita（μg）
1	30mg Pork	1.886	2.037	113.1	11.31
2		1.897	2.058	113.4	11.34
Média		1.892	2.048	113.25	11.325
3	20mg Shrimp	1.889	2.06	139.5	13.95
4		1.894	2.064	141.05	14.105
Média		1.892	2.062	140.275	14.028
5	30mg Beef	1.837	2.001	135.2	13.52
6		1.842	2.037	135	13.5
Média		1.834	2.019	135.1	13.51
7	30mg Mutton	1.851	2.043	175.75	17.575
8		1.84	2.073	180.25	18.025
Média		1.846	2.058	178	17.8
9	20mg Chicken	1.889	2.06	139.5	13.95
10		1.894	2.064	141.05	14.105
Média		1.892	2.062	140.275	14.028
11	50mg Fish	1.873	2.097	237	23.7
12		1.875	2.083	233.2	23.32
Média		1.874	2.09	235.1	23.51
13	3mg Fish heart	1.827	2.236	140.4	14.04
14		1.818	2.201	145.7	14.57
Média		1.823	2.219	143.05	14.305
15	3mg Fish liver	1.791	2.295	293.3	29.33
16		1.781	2.308	326.7	32.67
Média		1.786	2.302	310	31

Peak pattern：

Pork：

Shrimp：

Beef：

Mutton：

Chicken：

Fish：

Fish heart：

Fish liver：

O eletroferograma é mostrado abaixo：

M:Marker（2K，1K，750bp，500bp，250bp，100bp）;

1-2：30mg Pork 3-4：20mg Shrimp 5-6：30mg Beef 7-8：30mg Mutton

9-10：20mg Chicken 11-12：50mg Fish 13-14：3mg Fish heart 15-16：3mg Fish liver

Analysis of result：

The test results of the ultra-micro nucleic acid analyzer showed that:

①The value of A260/A280 is 1.78-1.9, and the value of A260/A230 is above 2.0, and the peak shape is complete and basically coincides, which proves that the purity of the extracted product is high.

②The yield of nucleic acid extraction from different animal tissue samples was more than 11 μg.

The results of agarose gel electrophoresis showed that:

①Tissue samples from different animals all had bright bands and good integrity.

Resumindo: the automatic nucleic acid extraction and purification instrument was used to extract different animal samples, and the extracted products had high purity, alto rendimento, complete electrophoresis bands, and the reagents and instruments were uniform and stable.

【Área de aplicação】

Genotyping of fresh or frozen animal tissues and cultured cells, high-throughput PCR screening of large-scale biological samples, transgene screening, knockout analysis and sequencing, enzyme digestion, PCR, Southern-Blot and library construction and other experiments.

Introdução ao fornecedor

Xangai Lingjun Biotecnologia Co., Ltda.foi estabelecido em 2016 e é um fabricante profissional de materiais biomagnéticos e reagentes de extração de ácido nucleico.

Temos vasta experiência em extração e purificação de ácidos nucleicos, purificação de proteínas, separação celular, quimioluminescência e outros campos técnicos.

Nossos produtos são amplamente utilizados em muitos campos, como exames médicos, testes genéticos, pesquisa universitária, melhoramento genético, e assim por diante. Nós não apenas fornecemos produtos, mas também podemos realizar OEM, ODM, e outras necessidades. Se você tiver necessidades relacionadas, não hesite em contactar-nos em sales01@lingjunbio.com.