無血清/血漿 DNA 抽出キット(磁気ビーズ法) 231003

【商品紹介】

The lysis solution in this product not only plays a role in lysing samples such as serum/plasma, 磁性ビーズが核酸を選択的に吸着するための環境も提供します。, したがって、溶解と結合をワンステップで実現します. The small amount of impurities adsorbed by the magnetic beads is removed by washing with the washing solution. 磁気ビーズは、溶出液の作用により吸着した核酸を放出します。, つまり, high quality free (ctDNA, cfDNA, ccfDNA) is obtained. The free DNA extracted by this product does not require toxic reagents such as phenol and chloroform, and the free DNA obtained can be used directly in downstream experiments.

【製品の利点】

1、The extraction effect is good, 特に小さな断片や微量 DNA の場合, 高い回収率を達成できる.

2、No need to add proteinase K in the extraction process, can be transported and stored at room temperature.

3、Cleavage and binding in one step, no need to pause in the middle to add other reagents.

4、strong versatility, can be used with different manufacturers of automated nucleic acid extraction equipment.

5、can adjust the experiment according to customer needs, to meet the extraction of large volume samples.

6、Good multi-sample compatibility, can be applied to a variety of sample types, 血などの, 血清, プラズマ, 唾液, body fluids and so on.

【商品画像】

【実験事例】

場合 1: A 100bp exogenous nucleic acid fragment was added to serum for simulation, サンプルが抽出され、qPCR が行われました。.

結果分析：It shows that the reagent system extracts nucleic acids with good purity and reproducibility.

電気泳動の結果は以下の通りでした, サンプル開始ボリューム: 200 μL, 80 溶出μL, 6 μL 上部サンプル電気泳動, アガロースゲルの濃度 1%, 120 V, 電気泳動用 30 分.

結果分析：上の図から分かるように, the experimental results are reproducible and can meet the experimental requirements of downstream qPCR, ddPCR とハイスループット シーケンス.

【適用範囲】

1、Tumor diagnosis and surveillance

It is used to detect circulating tumor DNA (ctDNA), which can analyze tumor gene mutations (e. g., EGFR, KRAS), copy number variation and epigenetic modification, and assist early cancer screening, efficacy evaluation and recurrence monitoring. 例えば, targeted therapy responsiveness was assessed by dynamic changes in ctDNA.

Non-invasive prenatal testing (NIPT)

Fetal free DNA is extracted from maternal plasma and combined with high-throughput sequencing technology to screen for chromosomal aneuploidy abnormalities such as Down syndrome to avoid the risk of traditional invasive testing.

Pathogen infection detection

Suitable for nucleic acid testing of viruses (e. g. HBV, HPV), bacterial or fungal infectious diseases, especially for the rapid capture of pathogen free DNA in the plasma of sepsis or immunosuppressed patients to improve diagnostic sensitivity.

Genetic analysis

DNA extraction for microdegradation samples, combined with gene mutation detection technology, can analyze the carrier status of genetic diseases.

サプライヤー紹介

上海霊軍生物技術有限公司, 株式会社に設立されました 2016 生体磁性材料と核酸抽出試薬の専門メーカーです.

核酸抽出・精製の経験が豊富です。, タンパク質の精製, 細胞分離, 化学発光およびその他の技術分野.

当社の製品はさまざまな分野で広く使用されています, 医療検査など, 遺伝子検査, 大学の研究, 遺伝子育種, 等々. 製品のご提供だけでなく、OEMも承ります。, ODM, その他のニーズ. 関連するニーズがある場合, sales01@lingjunbio.comまでお気軽にお問い合わせください。.