Serum/plasmafritt DNA-ekstraksjonssett(Magnetisk perlemetode) 231003

【Produktintroduksjon】

The lysis solution in this product not only plays a role in lysing samples such as serum/plasma, but also provides an environment for the magnetic beads to selectively adsorb nucleic acids, thus realizing lysis and binding in one step. The small amount of impurities adsorbed by the magnetic beads is removed by washing with the washing solution. The magnetic beads release the adsorbed nucleic acids under the action of the elution solution, dvs., high quality free (ctDNA, cfDNA, ccfDNA) er oppnådd. The free DNA extracted by this product does not require toxic reagents such as phenol and chloroform, and the free DNA obtained can be used directly in downstream experiments.

【Produktfordeler】

1、The extraction effect is good, especially for small fragments and trace DNA, can achieve a high recovery rate.

2、Det er ikke nødvendig å tilsette proteinase K i ekstraksjonsprosessen, can be transported and stored at room temperature.

3、Cleavage and binding in one step, no need to pause in the middle to add other reagents.

4、strong versatility, kan brukes med forskjellige produsenter av automatisert nukleinsyreekstraksjonsutstyr.

5、can adjust the experiment according to customer needs, to meet the extraction of large volume samples.

6、Good multi-sample compatibility, can be applied to a variety of sample types, som blod, serum, plasma, spytt, body fluids and so on.

【Produktbilde】

【Eksperimentelle tilfeller】

Sak 1: A 100bp exogenous nucleic acid fragment was added to serum for simulation, and samples were extracted and subjected to qPCR.

Resultatanalyse：Den viser at reagenssystemet ekstraherer nukleinsyrer med god renhet og reproduserbarhet.

Elektroforeseresultatene var som følger, prøvestartvolum: 200 μL, 80 μL eluering, 6 μL toppprøveelektroforese, agarose gel konsentrasjon av 1%, 120 V, elektroforese for 30 min.

Resultatanalyse：Som det fremgår av figuren ovenfor, de eksperimentelle resultatene er reproduserbare og kan oppfylle de eksperimentelle kravene til nedstrøms qPCR, ddPCR og high-throughput sekvensering.

【Bruksområde】

1、Tumor diagnosis and surveillance

It is used to detect circulating tumor DNA (ctDNA), which can analyze tumor gene mutations (e. g., EGFR, KRAS), copy number variation and epigenetic modification, and assist early cancer screening, efficacy evaluation and recurrence monitoring. For eksempel, targeted therapy responsiveness was assessed by dynamic changes in ctDNA.

Non-invasive prenatal testing (NIPT)

Fetal free DNA is extracted from maternal plasma and combined with high-throughput sequencing technology to screen for chromosomal aneuploidy abnormalities such as Down syndrome to avoid the risk of traditional invasive testing.

Pathogen infection detection

Suitable for nucleic acid testing of viruses (e. g. HBV, HPV), bacterial or fungal infectious diseases, especially for the rapid capture of pathogen free DNA in the plasma of sepsis or immunosuppressed patients to improve diagnostic sensitivity.

Genetic analysis

DNA extraction for microdegradation samples, combined with gene mutation detection technology, can analyze the carrier status of genetic diseases.

Leverandørintroduksjon

Shanghai Lingjun Biotechnology Co., Ltd.ble etablert i 2016 og er en profesjonell produsent av biomagnetiske materialer og nukleinsyreekstraksjonsreagenser.

Vi har rik erfaring innen utvinning og rensing av nukleinsyre, proteinrensing, celleseparasjon, kjemiluminescens og andre tekniske felt.

Våre produkter er mye brukt på mange felt, som medisinsk testing, genetisk testing, universitetsforskning, genetisk avl, og så videre. Vi leverer ikke bare produkter, men kan også ta OEM, ODM, og andre behov. Hvis du har relaterte behov, Ta gjerne kontakt med oss ​​på sales01@lingjunbio.com.