Serum / Plasma Free DNA Extraktioun Kit(Magnéitesch Bead Method) 231003

【Produktintroduktioun】

The lysis solution in this product not only plays a role in lysing samples such as serum/plasma, awer bitt och en Ëmfeld fir déi magnetesch Perlen fir selektiv Nukleinsäuren ze adsorbéieren, also realiséiere Lysis a Bindung an engem Schrëtt. The small amount of impurities adsorbed by the magnetic beads is removed by washing with the washing solution. Déi magnetesch Perlen befreien déi adsorbéiert Nukleinsäuren ënner der Handlung vun der Elutiounsléisung, i.e., high quality free (ctDNA, cfDNA, ccfDNA) is obtained. The free DNA extracted by this product does not require toxic reagents such as phenol and chloroform, and the free DNA obtained can be used directly in downstream experiments.

【Produktvirdeeler】

1、The extraction effect is good, besonnesch fir kleng Fragmenter a Spuer DNA, kann eng héich Erhuelung Taux erreechen.

2、Kee Grond fir Proteinase K am Extraktiounsprozess ze addéieren, can be transported and stored at room temperature.

3、Spaltung a Bindung an engem Schrëtt, no need to pause in the middle to add other reagents.

4、strong versatility, kann mat verschiddene Hiersteller vun automatiséierter Nukleinsäure Extraktioun Ausrüstung benotzt ginn.

5、can adjust the experiment according to customer needs, to meet the extraction of large volume samples.

6、Good multi-sample compatibility, kann op eng Vielfalt vu Probetypen applizéiert ginn, wéi Blutt, serum, Plasma, Spaut, Kierperflëssegkeeten a sou weider.

【Produktbild】

【Experimentell Fäll】

Fall 1: A 100bp exogenous nucleic acid fragment was added to serum for simulation, a Echantillon goufen extrahéiert an qPCR ënnerworf.

Resultat Analyse：It shows that the reagent system extracts nucleic acids with good purity and reproducibility.

D'Resultater vun der Elektrophorese waren wéi follegt, Prouf Startvolumen: 200 μL, 80 μL elutioun, 6 μL Top Probe Elektrophorese, agarose gelies Konzentratioun vun 1%, 120 V, Elektrophorese fir 30 min.

Resultat Analyse：Wéi kann aus der uewen Figur gesi ginn, déi experimentell Resultater sinn reproduzéierbar a kënnen den experimentellen Ufuerderunge vum Downstream qPCR treffen, ddPCR an High-Throughput Sequencing.

【Uwendungsberäich】

1、Tumor diagnosis and surveillance

It is used to detect circulating tumor DNA (ctDNA), which can analyze tumor gene mutations (e. g., EGFR, KRAS), copy number variation and epigenetic modification, and assist early cancer screening, efficacy evaluation and recurrence monitoring. Zum Beispill, targeted therapy responsiveness was assessed by dynamic changes in ctDNA.

Non-invasive prenatal testing (NIPT)

Fetal free DNA is extracted from maternal plasma and combined with high-throughput sequencing technology to screen for chromosomal aneuploidy abnormalities such as Down syndrome to avoid the risk of traditional invasive testing.

Pathogen infection detection

Suitable for nucleic acid testing of viruses (e. g. HBV, HPV), bacterial or fungal infectious diseases, especially for the rapid capture of pathogen free DNA in the plasma of sepsis or immunosuppressed patients to improve diagnostic sensitivity.

Genetic analysis

DNA extraction for microdegradation samples, combined with gene mutation detection technology, can analyze the carrier status of genetic diseases.

Fournisseur Aféierung

Shanghai Lingjun Biotechnology Co., Ltd., Ltd.gouf etabléiert an 2016 an ass e professionnelle Hiersteller vu biomagnetesche Materialien an Nukleinsäure Extraktiounsreagenz.

Mir hu räich Erfahrung an Nukleinsäure Extraktioun an Offäll, Protein Reinigung, Zell Trennung, Chemilumineszenz an aner technesch Felder.

Eis Produkter gi wäit a ville Beräicher benotzt, wéi medizinesch Tester, genetesch Tester, Universitéit Fuerschung, genetesch Zucht, an esou weider. Mir liwweren net nëmmen Produkter awer kënnen och OEM maachen, ODM, an aner Besoinen. Wann Dir verbonne Besoinen hutt, w.e.g. kontaktéiert eis op sales01@lingjunbio.com.