Lnjnbio Magnetic Beads for Stool Total DNA Extraction Kit 221005

(磁気ビーズ法)

【Product Introduction】

This product is suitable for the rapid and efficient extraction of total DNA from stool samples. The extraction process uses Digestive Buffer and Proteinase K to lyses the intestinal flora cells in the stool, releasing the total DNA. The magnetic beads selectively adsorb the total DNA in the lysate under the action of the binding buffer. The small amount of impurities adsorbed by the magnetic beads is removed by the Washing Buffer. The magnetic beads release the adsorbed total DNA under the action of the Elution Buffer, and the high-quality total DNA of intestinal flora can be obtained. The total DNA of intestinal flora extracted by this product does not need toxic reagents such as phenol, クロロホルム, 等, and the total DNA obtained can be directly used in downstream experiments.

【Product advantages】

1. High extraction yield: 10-60 μg of total DNA can be extracted from 200 mg of sample.

2. 幅広い適用性: suitable for solid or liquid stool samples from different sources.

3. Easy to operate: the extraction process can be completed within 30 min, also suitable for high-throughput operation through the automated workstation.

4. High safety: free of toxic reagents such as phenol and chloroform.

【Product image】

【Experimental cases】

場合 1:

Total DNA was extracted from six stool samples of different sources (sampling volume of 200 mg) using theStool Total DNA Extraction Kit from Shanghai Lingjun Biotechnology Co. 株式会社. on an automated nucleic acid extraction and purification instrument, LJbio32H, and about 10 μg of total DNA could be captured with high stability.

Sample number	あ260/あ280	あ260/あ230	濃度. (ng/μL)
1	1.76	1.91	105.6
2	1.75	1.69	82.6
3	1.77	2.39	114.9
4	1.75	1.88	107.6
56	1.77	2.43	116.4
6	1.77	2.46	115.8

場合 2：

The same stool sample was extracted multiple times (10 repetitions) using the Stool Total DNA Extraction Kit from Shanghai Lingjun Biotechnology Co., 株式会社. and quantitatively amplified by qPCR to detect 16S rRNA targets.

Result analysis：

The results showed that the Ct values for multiple extracted samples were essentially the same, thus indicating good reproducibility of the extraction kit.

【あpplication area】

Gut microbiome research: extract bacterial, fungal, and viral DNA / RNA, support 16S rRNA and metagenomic sequencing, and analyze the association between bacterial flora and disease.

Diagnosis of infectious diseases: detection of rotavirus, norovirus, Clostridium difficile, and other pathogens, to improve the efficiency of diarrhea pathogen identification.

Early tumor screening and precision medicine: extraction of fecal ctDNA, screening of genetic mutations and methylation markers of colorectal cancer, to achieve non-invasive early diagnosis.

Genetic diseases and metabolism research: analysis of host DNA and microflora genes, research of metabolic diseases mechanism and personalized intervention strategies.

Animal health and veterinary diagnosis: detect livestock and pet pathogens, optimize disease prevention and control, and diagnosis and treatment plan.

Environmental and food safety monitoring: tracking the pathogenic bacteria and drug resistance genes in water sources and food, to ensure public health safety.

サプライヤー紹介

上海霊軍生物技術有限公司, 株式会社に設立されました 2016 and is a professional manufacturer of biomagnetic materials and nucleic acid extraction reagents.

核酸抽出・精製の経験が豊富です。, タンパク質の精製, 細胞分離, 化学発光およびその他の技術分野.

当社の製品はさまざまな分野で広く使用されています, 医療検査など, 遺伝子検査, 大学の研究, 遺伝子育種, 等々. 製品のご提供だけでなく、OEMも承ります。, ODM, その他のニーズ. 関連するニーズがある場合, sales01@lingjunbio.comまでお気軽にお問い合わせください。.